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Article

Pollen Typhae total flavone improves insulin-induced glucose uptake through the β-arrestin-2-mediated signaling in C2C12 myotubes

  • Authors:
    • Xiao-Tao Feng
    • Tian-Zhan Wang
    • Yi Chen
    • Ji-Bo Liu
    • Yi Liu
    • Wen-Jian Wang
  • View Affiliations / Copyright

    Affiliations: Institute of Chinese Integrative Medicine, Huashan Hospital, Fudan University, Shanghai, P.R. China, Department of Endocrinology, Longhua Hospital, Shanghai University of Chinese Medicine, Shanghai, P.R. China
  • Pages: 914-922
    |
    Published online on: July 16, 2012
       https://doi.org/10.3892/ijmm.2012.1061
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Abstract

Defects in insulin-stimulated glucose uptake in skeletal muscle result from the dysfunction of insulin signaling including the phosphatidylinositol-3 kinase (PI3K) pathway and a novel β-arrestin-2-mediated signaling, which leads to insulin resistance (IR). Pollen Typhae, a Chinese herb, has been used for thousands of years in traditional Chinese medicine, and has the potential to inhibit the development of IR. We have previously reported that Pollen Typhae total flavone (PTF), the extract from Pollen Typhae, ameliorates high-glucose- and high-insulin-induced impairment of glucose uptake in 3T3-L1 adipocytes, but the mechanisms remain unclear. The objective of this study was to investigate the effects of PTF on glucose uptake, and to explore the underlying mechanisms in C2C12 myotubes. PTF improved insulin-stimulated glucose uptake in a dose- and time-dependent manner in C2C12 myotubes, and prevented palmitate-induced IR. Furthermore, PTF enhanced the basal gene expression of Src and Akt2, elevated the protein expression of β-arrestin-2, Src and Akt, increased the phosphorylation of insulin receptor-β at Tyr1150/1151 and Akt at Thr308/Ser473 in an insulin-dependent manner, but had no effects on the protein expression of PI3K-p85 or the activity of PI3K. Inhibition of Src but not PI3K restrained PTF-induced phosphorylation of Akt and glucose uptake. Our findings indicate that PTF improves insulin-induced glucose uptake via the β-arrestin-2-mediated signaling in C2C12 myotubes.
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Copy and paste a formatted citation
Spandidos Publications style
Feng X, Wang T, Chen Y, Liu J, Liu Y and Wang W: Pollen Typhae total flavone improves insulin-induced glucose uptake through the β-arrestin-2-mediated signaling in C2C12 myotubes. Int J Mol Med 30: 914-922, 2012.
APA
Feng, X., Wang, T., Chen, Y., Liu, J., Liu, Y., & Wang, W. (2012). Pollen Typhae total flavone improves insulin-induced glucose uptake through the β-arrestin-2-mediated signaling in C2C12 myotubes. International Journal of Molecular Medicine, 30, 914-922. https://doi.org/10.3892/ijmm.2012.1061
MLA
Feng, X., Wang, T., Chen, Y., Liu, J., Liu, Y., Wang, W."Pollen Typhae total flavone improves insulin-induced glucose uptake through the β-arrestin-2-mediated signaling in C2C12 myotubes". International Journal of Molecular Medicine 30.4 (2012): 914-922.
Chicago
Feng, X., Wang, T., Chen, Y., Liu, J., Liu, Y., Wang, W."Pollen Typhae total flavone improves insulin-induced glucose uptake through the β-arrestin-2-mediated signaling in C2C12 myotubes". International Journal of Molecular Medicine 30, no. 4 (2012): 914-922. https://doi.org/10.3892/ijmm.2012.1061
Copy and paste a formatted citation
x
Spandidos Publications style
Feng X, Wang T, Chen Y, Liu J, Liu Y and Wang W: Pollen Typhae total flavone improves insulin-induced glucose uptake through the β-arrestin-2-mediated signaling in C2C12 myotubes. Int J Mol Med 30: 914-922, 2012.
APA
Feng, X., Wang, T., Chen, Y., Liu, J., Liu, Y., & Wang, W. (2012). Pollen Typhae total flavone improves insulin-induced glucose uptake through the β-arrestin-2-mediated signaling in C2C12 myotubes. International Journal of Molecular Medicine, 30, 914-922. https://doi.org/10.3892/ijmm.2012.1061
MLA
Feng, X., Wang, T., Chen, Y., Liu, J., Liu, Y., Wang, W."Pollen Typhae total flavone improves insulin-induced glucose uptake through the β-arrestin-2-mediated signaling in C2C12 myotubes". International Journal of Molecular Medicine 30.4 (2012): 914-922.
Chicago
Feng, X., Wang, T., Chen, Y., Liu, J., Liu, Y., Wang, W."Pollen Typhae total flavone improves insulin-induced glucose uptake through the β-arrestin-2-mediated signaling in C2C12 myotubes". International Journal of Molecular Medicine 30, no. 4 (2012): 914-922. https://doi.org/10.3892/ijmm.2012.1061
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