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International Journal of Molecular Medicine
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Article

Application of a proteomic approach to identify proteins associated with primary graft non-function after liver transplantation

  • Authors:
    • Oskar Kornasiewicz
    • Kamil Bojarczuk
    • Marek Bugajski
    • Jakub Golab
    • Marek Krawczyk
  • View Affiliations / Copyright

    Affiliations: Chair and Department of General, Transplant and Liver Surgery, Medical University of Warsaw, 02-097 Warsaw, Poland, Department of Immunology, Center of Biostructure Research, Medical University of Warsaw, 02-097 Warsaw, Poland
  • Pages: 755-764
    |
    Published online on: July 16, 2012
       https://doi.org/10.3892/ijmm.2012.1062
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Abstract

Primary graft non-function (PNF) is a rare, life-threatening complication of liver transplantation. Increasing use of extended criteria donor pools and high-risk recipients seem to influence the incidence of PNF. Primary failure is associated with high patient morbidity and inferior graft survival. The only available treatment for PNF is emergency hepatic retransplantation, which is also correlated with significant morbidity and mortality. Therefore, researchers are working to identify risk factors of diagnostic value to prevent PNF. The current study attempted to explore liver proteomic patterns in patients with PNF. Using two-dimensional gel electrophoresis and liquid chromatography-mass spectrometry (LC-MS), we compared liver protein homogenates from 3 patients with PNF to those obtained from 6 healthy liver samples to identify potential new biomarkers of PNF. Our comparisons revealed 21 proteins with differential expression (13 upregulated and 8 downregulated). Most of these proteins are involved in energy metabolism, lipid metabolism, peptide cleavage, cell differentiation, and apoptosis. Although none of these proteins appeared more than once in separate analyses, this preliminary study shows that two-dimensional gel electrophoresis and LC-MS may allow identification of characteristic proteins to be used as biomarkers of a life-threatening complication of liver transplantation. Larger-scale analyses could improve patient care by finding suitable prognostic and therapeutic options. These data represent the first global proteomic approach to study PNF.
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1. 

RJ PloegAM D’AlessandroSJ KnechtleRisk factors for primary dysfunction after liver transplantation-a multivariate analysisTransplantation55807813199310.1097/00007890-199304000-000248475556

2. 

HY YooA MaheshwariPJ ThuluvathRetransplantation of liver: primary graft nonfunction and hepatitis C virus are associated with worse outcomeLiver Transpl9897904200310.1053/jlts.2003.5017612942450

3. 

T UemuraHB RandallEQ SanchezLiver retransplantation for primary nonfunction: analysis of a 20-year single-center experienceLiver Transpl13227233200717256780

4. 

BW Shaw JrRD GordonS IwatsukiTE StarzlRetransplantation of the liverSemin Liver Dis5394401198510.1055/s-2008-1040638

5. 

GR SilberhumerH PokornyH HetzCombination of extended donor criteria and changes in the Model for End-Stage Liver Disease score predict patient survival and primary dysfunction in liver transplantation: a retrospective analysisTransplantation83588592200710.1097/01.tp.0000255319.07499.b7

6. 

MG AminMP WolfJA TenBrook JrExpanded criteria donor grafts for deceased donor liver transplantation under the MELD system: a decision analysisLiver Transpl1014681475200410.1002/lt.20304

7. 

N KemmerM SecicV ZachariasT KaiserGW NeffLong-term analysis of primary nonfunction in liver transplant recipientsTransplant Proc3914771480200710.1016/j.transproceed.2006.11.01217580166

8. 

AK BurroughsCA SabinK Rolles3-month and 12-month mortality after first liver transplant in adults in Europe: predictive models for outcomeLancet367225232200610.1016/S0140-6736(06)68033-116427491

9. 

R AdamP McMasterJG O’GradyEvolution of liver transplantation in Europe: report of the European Liver Transplant RegistryLiver Transpl912311243200310.1016/j.lts.2003.09.01814625822

10. 

J PitreO SoubraneB DoussetHow valid is emergency liver transplantation for acute liver necrosis in patients with multiple-organ failure?Liver Transpl Surg217199610.1002/lt.5000201029346621

11. 

HR DoyleF MorelliJ McMichaelHepatic retransplantation - an analysis of risk factors associated with outcomeTransplantation6114991505199610.1097/00007890-199605270-000168633379

12. 

KJ OldhaferA BornscheuerNR FrühaufRescue hepatectomy for initial graft non-function after liver transplantationTransplantation6710241028199910.1097/00007890-199904150-0001510221488

13. 

SK SoJA BarteauGA PerdrizetJW MarshSuccessful retransplantation after a 48-hour anhepatic stateTransplant Proc251962196319938385827

14. 

H ChenCH PengBY ShenMulti-factor analysis of initial poor graft function after orthotopic liver transplantationHepatobiliary Pancreat Dis Int6141146200717374571

15. 

JR LakeJS ShorrBJ SteffenAH ChuRD GordonRH WiesnerDifferential effects of donor age in liver transplant recipients infected with hepatitis B, hepatitis C and without viral hepatitisAm J Transplant5549557200510.1111/j.1600-6143.2005.00741.x15707410

16. 

S FengNP GoodrichJL Bragg-GreshamCharacteristics associated with liver graft failure: the concept of a donor risk indexAm J Transplant6783790200610.1111/j.1600-6143.2006.01242.x16539636

17. 

PA ClavienM SelznerHA RüdigerA prospective randomized study in 100 consecutive patients undergoing major liver resection with versus without ischemic preconditioningAnn Surg238843852200310.1097/01.sla.0000098620.27623.7d

18. 

SM StrasbergTK HowardEP MolmentiM HertlSelecting the donor liver: risk factors for poor function after orthotopic liver transplantationHepatology20829838199410.1002/hep.18402004107927223

19. 

E TotsukaF DodsonA UrakamiInfluence of high donor serum sodium levels on early postoperative graft function in human liver transplantation: effect of correction of donor hypernatremiaLiver Transpl Surg5421428199910.1002/lt.500050510

20. 

H YersizA ShakedK OlthoffCorrelation between donor age and the pattern of liver graft recovery after transplantationTransplantation60790794199510.1097/00007890-199510270-000057482736

21. 

AM CameronRM GhobrialH YersizOptimal utilization of donor grafts with extended criteria: a single-center experience in over 1000 liver transplantsAnn Surg243748755200610.1097/01.sla.0000219669.84192.b316772778

22. 

SR JohnsonS AlexopoulosM CurryDW HantoPrimary nonfunction (PNF) in the MELD era: An SRTR database analysisAm J Transplant710031009200710.1111/j.1600-6143.2006.01702.x17286618

23. 

J BusquetsX XiolJ FiguerasThe impact of donor age on liver transplantation: influence of donor age on early liver function and on subsequent patient and graft survivalTransplantation7117651771200110.1097/00007890-200106270-0001111455256

24. 

WK WashburnLB JohnsonWD LewisRL JenkinsGraft function and outcome of older (> or = 60 years) donor liversTransplantation61106210661996

25. 

H PokornyT GruenbergerT SolimanS RockenschaubF LängleR SteiningerOrgan survival after primary dysfunction of liver grafts in clinical orthotopic liver transplantationTranspl Int13Suppl 1S154S157200010.1007/s00147005031011111986

26. 

B MüllhauptD DimitroulisJT GerlachPA ClavienHot topics in liver transplantation: organ allocation - extended criteria donor - living donor liver transplantationJ Hepatol48Suppl 1S58S67200818308415

27. 

HY ChungSC ChanCM LoST FanStrategies for widening liver donor poolAsian J Surg336369201010.1016/S1015-9584(10)60011-521029941

28. 

RW BusuttilK TanakaThe utility of marginal donors in liver transplantationLiver Transpl9651663200310.1053/jlts.2003.5010512827549

29. 

M GastacaExtended criteria donors in liver transplantation: adapting donor quality and recipientTransplant Proc41975979200910.1016/j.transproceed.2009.02.01619376402

30. 

K HatsugaiN OhkohchiT FukumoriY AkamatsuS SatomiMechanism of primary graft non-function in a rat model for fatty liver transplantationTranspl Int13Suppl 1S583S590200010.1007/s00147005040811112079

31. 

D AzoulayMM LinharesE HuguetDecision for retransplantation of the liver: an experience- and cost-based analysisAnn Surg236713721200210.1097/00000658-200212000-0000312454509

32. 

C VascottoL CesarattoC D’AmbrosioProteomic analysis of liver tissues subjected to early ischemia/reperfusion injury during human orthotopic liver transplantationProteomics634553465200610.1002/pmic.200500770

33. 

M ChevalletS LucheT RabilloudSilver staining of proteins in polyacrylamide gelsNat Protoc118521858200610.1038/nprot.2006.28817487168

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Copy and paste a formatted citation
Spandidos Publications style
Kornasiewicz O, Bojarczuk K, Bugajski M, Golab J and Krawczyk M: Application of a proteomic approach to identify proteins associated with primary graft non-function after liver transplantation. Int J Mol Med 30: 755-764, 2012.
APA
Kornasiewicz, O., Bojarczuk, K., Bugajski, M., Golab, J., & Krawczyk, M. (2012). Application of a proteomic approach to identify proteins associated with primary graft non-function after liver transplantation. International Journal of Molecular Medicine, 30, 755-764. https://doi.org/10.3892/ijmm.2012.1062
MLA
Kornasiewicz, O., Bojarczuk, K., Bugajski, M., Golab, J., Krawczyk, M."Application of a proteomic approach to identify proteins associated with primary graft non-function after liver transplantation". International Journal of Molecular Medicine 30.4 (2012): 755-764.
Chicago
Kornasiewicz, O., Bojarczuk, K., Bugajski, M., Golab, J., Krawczyk, M."Application of a proteomic approach to identify proteins associated with primary graft non-function after liver transplantation". International Journal of Molecular Medicine 30, no. 4 (2012): 755-764. https://doi.org/10.3892/ijmm.2012.1062
Copy and paste a formatted citation
x
Spandidos Publications style
Kornasiewicz O, Bojarczuk K, Bugajski M, Golab J and Krawczyk M: Application of a proteomic approach to identify proteins associated with primary graft non-function after liver transplantation. Int J Mol Med 30: 755-764, 2012.
APA
Kornasiewicz, O., Bojarczuk, K., Bugajski, M., Golab, J., & Krawczyk, M. (2012). Application of a proteomic approach to identify proteins associated with primary graft non-function after liver transplantation. International Journal of Molecular Medicine, 30, 755-764. https://doi.org/10.3892/ijmm.2012.1062
MLA
Kornasiewicz, O., Bojarczuk, K., Bugajski, M., Golab, J., Krawczyk, M."Application of a proteomic approach to identify proteins associated with primary graft non-function after liver transplantation". International Journal of Molecular Medicine 30.4 (2012): 755-764.
Chicago
Kornasiewicz, O., Bojarczuk, K., Bugajski, M., Golab, J., Krawczyk, M."Application of a proteomic approach to identify proteins associated with primary graft non-function after liver transplantation". International Journal of Molecular Medicine 30, no. 4 (2012): 755-764. https://doi.org/10.3892/ijmm.2012.1062
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