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October 2012 Volume 30 Issue 4

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Article

Role of the p38 MAPK signaling pathway in mediating interleukin-28A-induced migration of UMUC-3 cells

  • Authors:
    • Se-Jung Lee
    • Wun-Jae Kim
    • Sung-Kwon Moon
  • View Affiliations / Copyright

    Affiliations: Department of Biotechnology, Chungju National University, Chungju, Chungbuk 380-702, Republic of Korea, Personalized Tumor Engineering Research Center, Department of Urology, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea
  • Pages: 945-952
    |
    Published online on: July 16, 2012
       https://doi.org/10.3892/ijmm.2012.1064
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Abstract

Although interleukin-28A (IL-28A) is believed to have an antiviral effect, its role in tumor migration requires further examination. The present study was intended to verify the effect of IL-28A on the migration of UMUC-3 bladder cancer cells. IL-28A and its receptor IL-28AR1 mRNA were detected in UMUC-3 cells. Although exogenous IL-28A showed no effect on cell proliferation, a wound-healing migration assay showed that the migration of UMUC-3 cells was induced by IL-28A. Furthermore, treatment of the cells with IL-28A significantly promoted MMP-9 expression via binding activities of NF-κB and AP-1. IL-28A also induced the activation of p38 MAPK and Jak2-Stat2 signaling. Using the p38 MAPK inhibitor SB203580 and the dominant-negative plasmid DN-p38, we found evidence that the inhibition of p38 MAPK signaling suppressed the effects of IL-28A including wound-healing migration and MMP-9 expression by activation of NF-κB and AP-1 binding in UMUC-3 cells. However, Jak-2 inhibition by AG490 did not affect IL-28A-induced migration of UMUC-3 cells. Collectively, we suggest for the first time that the p38 MAPK pathway mediates IL-28A-induced cell migration through MMP-9 expression by activating NF-κB and AP-1 binding motifs.
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Copy and paste a formatted citation
Spandidos Publications style
Lee S, Kim W and Moon S: Role of the p38 MAPK signaling pathway in mediating interleukin-28A-induced migration of UMUC-3 cells. Int J Mol Med 30: 945-952, 2012.
APA
Lee, S., Kim, W., & Moon, S. (2012). Role of the p38 MAPK signaling pathway in mediating interleukin-28A-induced migration of UMUC-3 cells. International Journal of Molecular Medicine, 30, 945-952. https://doi.org/10.3892/ijmm.2012.1064
MLA
Lee, S., Kim, W., Moon, S."Role of the p38 MAPK signaling pathway in mediating interleukin-28A-induced migration of UMUC-3 cells". International Journal of Molecular Medicine 30.4 (2012): 945-952.
Chicago
Lee, S., Kim, W., Moon, S."Role of the p38 MAPK signaling pathway in mediating interleukin-28A-induced migration of UMUC-3 cells". International Journal of Molecular Medicine 30, no. 4 (2012): 945-952. https://doi.org/10.3892/ijmm.2012.1064
Copy and paste a formatted citation
x
Spandidos Publications style
Lee S, Kim W and Moon S: Role of the p38 MAPK signaling pathway in mediating interleukin-28A-induced migration of UMUC-3 cells. Int J Mol Med 30: 945-952, 2012.
APA
Lee, S., Kim, W., & Moon, S. (2012). Role of the p38 MAPK signaling pathway in mediating interleukin-28A-induced migration of UMUC-3 cells. International Journal of Molecular Medicine, 30, 945-952. https://doi.org/10.3892/ijmm.2012.1064
MLA
Lee, S., Kim, W., Moon, S."Role of the p38 MAPK signaling pathway in mediating interleukin-28A-induced migration of UMUC-3 cells". International Journal of Molecular Medicine 30.4 (2012): 945-952.
Chicago
Lee, S., Kim, W., Moon, S."Role of the p38 MAPK signaling pathway in mediating interleukin-28A-induced migration of UMUC-3 cells". International Journal of Molecular Medicine 30, no. 4 (2012): 945-952. https://doi.org/10.3892/ijmm.2012.1064
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