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Article

Identification of Pold2 as a novel interaction partner of protein inhibitor of activated STAT2

  • Authors:
    • Ying Zheng
    • Shudong Sheng
    • Haiyan Wang
    • Xiaoqing Jia
    • Yanqiu Hu
    • Yayun Qian
    • Yongze Zhu
    • Jianjun Wang
  • View Affiliations / Copyright

    Affiliations: Department of Histology and Embryology, Medical College, Yangzhou University, Yangzhou 225001, P.R. China, Department of Medicine, Yangzhou Vocational College of Environment and Resources, Yangzhou 225001, P.R. China, Center of Reproductive Medicine, Clinical Medical College, Yangzhou University, Yangzhou 225001, P.R. China
  • Pages: 884-888
    |
    Published online on: July 16, 2012
       https://doi.org/10.3892/ijmm.2012.1065
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Abstract

Pold2 is a subunit of the DNA polymerase δ complex, encoding a protein involved in DNA replication and repair. In this study, using a yeast two-hybrid screening technique and the common cDNA fragment of the mouse PIAS2 as a bait, Pold2 was found to interact with PIAS2. A direct interaction between Pold2 and PIAS2 was confirmed by direct yeast two-hybrid. In vivo evidence of Pold2 association with PIAS2 was obtained by co-immunoprecipitation using HEK-293 cells. Subcellular localization studies demonstrated that Pold2 and PIAS2 were partially co-localized in mammalian cells. Collectively, our results suggest that Pold2 interacts under physiological conditions with PIAS2.
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Copy and paste a formatted citation
Spandidos Publications style
Zheng Y, Sheng S, Wang H, Jia X, Hu Y, Qian Y, Zhu Y and Wang J: Identification of Pold2 as a novel interaction partner of protein inhibitor of activated STAT2. Int J Mol Med 30: 884-888, 2012.
APA
Zheng, Y., Sheng, S., Wang, H., Jia, X., Hu, Y., Qian, Y. ... Wang, J. (2012). Identification of Pold2 as a novel interaction partner of protein inhibitor of activated STAT2. International Journal of Molecular Medicine, 30, 884-888. https://doi.org/10.3892/ijmm.2012.1065
MLA
Zheng, Y., Sheng, S., Wang, H., Jia, X., Hu, Y., Qian, Y., Zhu, Y., Wang, J."Identification of Pold2 as a novel interaction partner of protein inhibitor of activated STAT2". International Journal of Molecular Medicine 30.4 (2012): 884-888.
Chicago
Zheng, Y., Sheng, S., Wang, H., Jia, X., Hu, Y., Qian, Y., Zhu, Y., Wang, J."Identification of Pold2 as a novel interaction partner of protein inhibitor of activated STAT2". International Journal of Molecular Medicine 30, no. 4 (2012): 884-888. https://doi.org/10.3892/ijmm.2012.1065
Copy and paste a formatted citation
x
Spandidos Publications style
Zheng Y, Sheng S, Wang H, Jia X, Hu Y, Qian Y, Zhu Y and Wang J: Identification of Pold2 as a novel interaction partner of protein inhibitor of activated STAT2. Int J Mol Med 30: 884-888, 2012.
APA
Zheng, Y., Sheng, S., Wang, H., Jia, X., Hu, Y., Qian, Y. ... Wang, J. (2012). Identification of Pold2 as a novel interaction partner of protein inhibitor of activated STAT2. International Journal of Molecular Medicine, 30, 884-888. https://doi.org/10.3892/ijmm.2012.1065
MLA
Zheng, Y., Sheng, S., Wang, H., Jia, X., Hu, Y., Qian, Y., Zhu, Y., Wang, J."Identification of Pold2 as a novel interaction partner of protein inhibitor of activated STAT2". International Journal of Molecular Medicine 30.4 (2012): 884-888.
Chicago
Zheng, Y., Sheng, S., Wang, H., Jia, X., Hu, Y., Qian, Y., Zhu, Y., Wang, J."Identification of Pold2 as a novel interaction partner of protein inhibitor of activated STAT2". International Journal of Molecular Medicine 30, no. 4 (2012): 884-888. https://doi.org/10.3892/ijmm.2012.1065
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