Open Access

Induction of apoptosis by laminarin, regulating the insulin-like growth factor-IR signaling pathways in HT-29 human colon cells

  • Authors:
    • Hee-Kyoung Park
    • In-Hye Kim
    • Joongkyun Kim
    • Taek-Jeong Nam
  • View Affiliations

  • Published online on: August 2, 2012     https://doi.org/10.3892/ijmm.2012.1084
  • Pages: 734-738
  • Copyright: © Park et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

In recent years, algae have been highlighted as potential sources of anticancer agents. Laminarin is a molecule found in marine brown algae that has potentially beneficial biological activities. However, these activities have not been investigated. In the present study, we examined the effects of laminarin on HT-29 cells and analyzed its effect on the insulin-like growth factor (IGF-IR) signaling pathway. 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxy-phenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assays revealed that laminarin induced cell death in a dose-dependent manner. Western blotting showed that laminarin decreased mitogen-activated protein kinases (MAPK) and ERK phosphorylation. Decreased proliferation depended on IGF-IR, which was associated with the downregulation of MAPK/ERK. These results are important for understanding the roles of IGF-IR in colon cancer cell tumorigenesis, and suggest that laminarin shows activity against human colon cancer.
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October 2012
Volume 30 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Park H, Kim I, Kim J and Nam T: Induction of apoptosis by laminarin, regulating the insulin-like growth factor-IR signaling pathways in HT-29 human colon cells. Int J Mol Med 30: 734-738, 2012.
APA
Park, H., Kim, I., Kim, J., & Nam, T. (2012). Induction of apoptosis by laminarin, regulating the insulin-like growth factor-IR signaling pathways in HT-29 human colon cells. International Journal of Molecular Medicine, 30, 734-738. https://doi.org/10.3892/ijmm.2012.1084
MLA
Park, H., Kim, I., Kim, J., Nam, T."Induction of apoptosis by laminarin, regulating the insulin-like growth factor-IR signaling pathways in HT-29 human colon cells". International Journal of Molecular Medicine 30.4 (2012): 734-738.
Chicago
Park, H., Kim, I., Kim, J., Nam, T."Induction of apoptosis by laminarin, regulating the insulin-like growth factor-IR signaling pathways in HT-29 human colon cells". International Journal of Molecular Medicine 30, no. 4 (2012): 734-738. https://doi.org/10.3892/ijmm.2012.1084