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Induction of apoptosis by laminarin, regulating the insulin-like growth factor-IR signaling pathways in HT-29 human colon cells

  • Authors:
    • Hee-Kyoung Park
    • In-Hye Kim
    • Joongkyun Kim
    • Taek-Jeong Nam
  • View Affiliations / Copyright

    Affiliations: Department of Food Science and Nutrition, Pukyong National University, Nam-gu, Busan 608-737, Republic of Korea, Department of Biotechnology, Pukyong National University, Nam-gu, Busan 608-737, Republic of Korea
    Copyright: © Park et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 734-738
    |
    Published online on: August 2, 2012
       https://doi.org/10.3892/ijmm.2012.1084
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Abstract

In recent years, algae have been highlighted as potential sources of anticancer agents. Laminarin is a molecule found in marine brown algae that has potentially beneficial biological activities. However, these activities have not been investigated. In the present study, we examined the effects of laminarin on HT-29 cells and analyzed its effect on the insulin-like growth factor (IGF-IR) signaling pathway. 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxy-phenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assays revealed that laminarin induced cell death in a dose-dependent manner. Western blotting showed that laminarin decreased mitogen-activated protein kinases (MAPK) and ERK phosphorylation. Decreased proliferation depended on IGF-IR, which was associated with the downregulation of MAPK/ERK. These results are important for understanding the roles of IGF-IR in colon cancer cell tumorigenesis, and suggest that laminarin shows activity against human colon cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Park H, Kim I, Kim J and Nam T: Induction of apoptosis by laminarin, regulating the insulin-like growth factor-IR signaling pathways in HT-29 human colon cells. Int J Mol Med 30: 734-738, 2012.
APA
Park, H., Kim, I., Kim, J., & Nam, T. (2012). Induction of apoptosis by laminarin, regulating the insulin-like growth factor-IR signaling pathways in HT-29 human colon cells. International Journal of Molecular Medicine, 30, 734-738. https://doi.org/10.3892/ijmm.2012.1084
MLA
Park, H., Kim, I., Kim, J., Nam, T."Induction of apoptosis by laminarin, regulating the insulin-like growth factor-IR signaling pathways in HT-29 human colon cells". International Journal of Molecular Medicine 30.4 (2012): 734-738.
Chicago
Park, H., Kim, I., Kim, J., Nam, T."Induction of apoptosis by laminarin, regulating the insulin-like growth factor-IR signaling pathways in HT-29 human colon cells". International Journal of Molecular Medicine 30, no. 4 (2012): 734-738. https://doi.org/10.3892/ijmm.2012.1084
Copy and paste a formatted citation
x
Spandidos Publications style
Park H, Kim I, Kim J and Nam T: Induction of apoptosis by laminarin, regulating the insulin-like growth factor-IR signaling pathways in HT-29 human colon cells. Int J Mol Med 30: 734-738, 2012.
APA
Park, H., Kim, I., Kim, J., & Nam, T. (2012). Induction of apoptosis by laminarin, regulating the insulin-like growth factor-IR signaling pathways in HT-29 human colon cells. International Journal of Molecular Medicine, 30, 734-738. https://doi.org/10.3892/ijmm.2012.1084
MLA
Park, H., Kim, I., Kim, J., Nam, T."Induction of apoptosis by laminarin, regulating the insulin-like growth factor-IR signaling pathways in HT-29 human colon cells". International Journal of Molecular Medicine 30.4 (2012): 734-738.
Chicago
Park, H., Kim, I., Kim, J., Nam, T."Induction of apoptosis by laminarin, regulating the insulin-like growth factor-IR signaling pathways in HT-29 human colon cells". International Journal of Molecular Medicine 30, no. 4 (2012): 734-738. https://doi.org/10.3892/ijmm.2012.1084
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