Glycosaminoglycan chains of biglycan promote bone morphogenetic protein-4-induced osteoblast differentiation

Ye,Yaping; Hu,Weihua; Guo,Fengjing; Zhang,Weikai; Wang,Jiang; Chen,Anmin

doi:10.3892/ijmm.2012.1091

November 2012 Volume 30 Issue 5

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November 2012 Volume 30 Issue 5

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Article

Glycosaminoglycan chains of biglycan promote bone morphogenetic protein-4-induced osteoblast differentiation

Authors:
- Yaping Ye
- Weihua Hu
- Fengjing Guo
- Weikai Zhang
- Jiang Wang
- Anmin Chen
View Affiliations / Copyright

Affiliations: Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, P.R. China
Pages: 1075-1080
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Published online on: August 9, 2012

https://doi.org/10.3892/ijmm.2012.1091
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Abstract

Biglycan (BGN) has been reported to promote bone morphogenetic protein-4 (BMP-4) stimulated osteoblastic differentiation. However, the underlying mechanism has yet to be fully elucidated. The glycosaminoglycan (GAG) chains of BGN have a variety of biological functions. In the present study, we explored the potential role of the GAG chains of BGN in promoting BMP-4-induced osteoblast differentiation. BGN knockout (KO) murine calvarial cells were transfected with adenovirus overexpressing wild-type BGN (Adv-BGN), adenovirus expressing GAG-mutant BGN (Adv-BGNm) and adenovirus without BGN (Adv-Emp). Transfected cells were treated with or without BMP-4. Subsequently, BMP-4 signaling and function were assessed by evaluating the expression of the osteoblast differentiation-related proteins, Smad1/5/8 phosphorylation and alkaline phosphatase (ALP) activity. Furthermore, the binding specificity of the transfected cells to BMP-4 was also investigated using immunofluorescence staining. Our study demonstrated that a mutant BGN lacking GAG chains decreased BGN-assisted BMP-4 signaling and osteoblast differentiation and that the expression of this mutant BGN in biglycan knockout (BGN‑KO) calvarial osteoblasts could not rescue its differentiation deficiency as efficiently as wild-type (WT) BGN. These results strongly suggest that the GAG chains of BGN promote BGN-assisted BMP-4 function.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Glycosaminoglycan chains of biglycan promote bone morphogenetic protein-4-induced osteoblast differentiation

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