Gastrodin inhibits cell proliferation in vascular smooth muscle cells and attenuates neointima formation in vivo

Zhu,Lihua; Guan,Hongjing; Cui,Changping; Tian,Song; Yang,Da; Wang,Xinan; Zhang,Shuming; Wang,Lang; Jiang,Hong

doi:10.3892/ijmm.2012.1100

November 2012 Volume 30 Issue 5

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November 2012 Volume 30 Issue 5

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Article Open Access

Gastrodin inhibits cell proliferation in vascular smooth muscle cells and attenuates neointima formation in vivo

Authors:
- Lihua Zhu
- Hongjing Guan
- Changping Cui
- Song Tian
- Da Yang
- Xinan Wang
- Shuming Zhang
- Lang Wang
- Hong Jiang
View Affiliations / Copyright

Affiliations: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China, Cardiovascular Research Institute of Wuhan University, Wuhan, Hubei 430060, P.R. China

Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
Pages: 1034-1040
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Published online on: August 20, 2012

https://doi.org/10.3892/ijmm.2012.1100
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Abstract

Vascular smooth muscle cell (VSMC) proliferation plays a critical role in the development of vascular diseases. In the present study, we tested the efficacy and the mechanisms of action of gastrodin, a bioactive component of the Chinese herb Gastrodia elata Bl, in relation to platelet-derived growth factor-BB (PDGF-BB)-dependent cell proliferation and neointima formation after acute vascular injury. Cell experiments were performed with VSMCs isolated from rat aortas. WST and BrdU incorporation assays were used to evaluate VSMC proliferation. Eight-week-old C57BL/6 mice were used for the animal experiments. Gastrodin (150 mg/kg/day) was administered in the animal chow for 14 days, and the mice were subjected to wire injury of the left carotid artery. Our data demonstrated that gastrodin attenuated the VSMC proliferation induced by PDGF-BB, as assessed by WST assay and BrdU incorporation. Gastrodin influenced the S-phase entry of VSMCs and stabilised p27Kip1 expression. In addition, pre-incubation with sinomenine prior to PDGF-BB stimulation led to increased smooth muscle-specific gene expression, thereby inhibiting VSMC dedifferentiation. Gastrodin treatment also reduced the intimal area and the number of PCNA-positive cells. Furthermore, PDGF-BB-induced phosphorylation of ERK1/2, p38 MAPK, Akt and GSK3β was suppressed by gastrodin. Our results suggest that gastrodin can inhibit VSMC proliferation and attenuate neointimal hyperplasia in response to vascular injury. Furthermore, the ERK1/2, p38 MAPK and Akt/GSK3β signalling pathways were found to be involved in the effects of gastrodin.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Gastrodin inhibits cell proliferation in vascular smooth muscle cells and attenuates neointima formation in vivo

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