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International Journal of Molecular Medicine
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November 2012 Volume 30 Issue 5

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Article

Antiproliferative and apoptotic effect of epigallocatechin-3-gallate on Ishikawa cells is accompanied by sex steroid receptor downregulation

  • Authors:
    • Seung Bin Park
    • Jong Woon Bae
    • Jong Min Kim
    • Seung Gee Lee
    • Myoungseok Han
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Dong-A University, College of Medicine, Busan 602-715, Republic of Korea, Department of Anatomy and Cell Biology, Dong-A University, College of Medicine, Busan 602-715, Republic of Korea
  • Pages: 1211-1218
    |
    Published online on: August 20, 2012
       https://doi.org/10.3892/ijmm.2012.1104
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Abstract

Endometrial cancer is a significant malignancy in developed countries. Unopposed estrogen stimulation is considered as an important risk factor for endometrial cancer. Epigallocathechin-3-gallate (EGCG), biological active component of green tea, inhibits cancer cell proliferation. However, it is unknown whether EGCG has anticancer effects on endometrial cancer and what the molecular mechanism(s) are. We investigated the anticancer effects of EGCG on a human endometrial adenocarcinoma cell line (Ishikawa cells) with or without 17β-estradiol (E2) treatment. Cell proliferation assay was performed using 3-(4,5-dimethylthiaxol-2-yi)-2,5-diphenyltetraxolium bromide (MTT). The cell cycle was determined by flow cytometry and real-time analysis of cyclin and cdk genes. The apoptosis was measured by Annexin V-PI staining and real-time analysis of bcl-2, Bax and caspase genes. The MAPK signal, Akt and caspase-3 were determined by immunoblotting. Decreased estrogen and progesterone receptor expression was observed in EGCG-treated Ishikawa cells, and decreased MAPK signals and phospho-Akt were observed as well. EGCG caused the arrest of cells in the G0/G1 phase of the cell cycle. This compound interfered with Akt activation and MAPK signals, and increased apoptosis signals leading to a controlled caspases, Bcl-2, Bax genes and protein expression. Taken together, EGCG inhibits cell proliferation and induces apoptosis through Akt and MAPK signals. These findings suggest that EGCG may exert growth-inhibitory and apoptosis-inducing effects on endometrial cancer cells, accompanied by decreased estrogen and progesterone receptor. EGCG may have future clinical implications with respect to the development of novel approaches as an adjuvant therapy in endometrial cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Park SB, Bae JW, Kim JM, Lee SG and Han M: Antiproliferative and apoptotic effect of epigallocatechin-3-gallate on Ishikawa cells is accompanied by sex steroid receptor downregulation. Int J Mol Med 30: 1211-1218, 2012.
APA
Park, S.B., Bae, J.W., Kim, J.M., Lee, S.G., & Han, M. (2012). Antiproliferative and apoptotic effect of epigallocatechin-3-gallate on Ishikawa cells is accompanied by sex steroid receptor downregulation. International Journal of Molecular Medicine, 30, 1211-1218. https://doi.org/10.3892/ijmm.2012.1104
MLA
Park, S. B., Bae, J. W., Kim, J. M., Lee, S. G., Han, M."Antiproliferative and apoptotic effect of epigallocatechin-3-gallate on Ishikawa cells is accompanied by sex steroid receptor downregulation". International Journal of Molecular Medicine 30.5 (2012): 1211-1218.
Chicago
Park, S. B., Bae, J. W., Kim, J. M., Lee, S. G., Han, M."Antiproliferative and apoptotic effect of epigallocatechin-3-gallate on Ishikawa cells is accompanied by sex steroid receptor downregulation". International Journal of Molecular Medicine 30, no. 5 (2012): 1211-1218. https://doi.org/10.3892/ijmm.2012.1104
Copy and paste a formatted citation
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Spandidos Publications style
Park SB, Bae JW, Kim JM, Lee SG and Han M: Antiproliferative and apoptotic effect of epigallocatechin-3-gallate on Ishikawa cells is accompanied by sex steroid receptor downregulation. Int J Mol Med 30: 1211-1218, 2012.
APA
Park, S.B., Bae, J.W., Kim, J.M., Lee, S.G., & Han, M. (2012). Antiproliferative and apoptotic effect of epigallocatechin-3-gallate on Ishikawa cells is accompanied by sex steroid receptor downregulation. International Journal of Molecular Medicine, 30, 1211-1218. https://doi.org/10.3892/ijmm.2012.1104
MLA
Park, S. B., Bae, J. W., Kim, J. M., Lee, S. G., Han, M."Antiproliferative and apoptotic effect of epigallocatechin-3-gallate on Ishikawa cells is accompanied by sex steroid receptor downregulation". International Journal of Molecular Medicine 30.5 (2012): 1211-1218.
Chicago
Park, S. B., Bae, J. W., Kim, J. M., Lee, S. G., Han, M."Antiproliferative and apoptotic effect of epigallocatechin-3-gallate on Ishikawa cells is accompanied by sex steroid receptor downregulation". International Journal of Molecular Medicine 30, no. 5 (2012): 1211-1218. https://doi.org/10.3892/ijmm.2012.1104
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