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International Journal of Molecular Medicine
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November 2012 Volume 30 Issue 5

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Article

The synergistic effects of low-dose irinotecan and TRAIL on TRAIL-resistant HT-29 colon carcinoma in vitro and in vivo

  • Authors:
    • Hong Zhu
    • Fen Zhao
    • Shuijing Yu
    • Jianping He
    • Licong Deng
    • Cheng Yi
    • Ying Huang
  • View Affiliations / Copyright

    Affiliations: Department of Abdominal Cancer, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Department of Pathophysiology, West China School of Preclinical Sciences and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, P.R. China
  • Pages: 1087-1094
    |
    Published online on: August 21, 2012
       https://doi.org/10.3892/ijmm.2012.1105
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Abstract

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a member of the tumor necrosis factor superfamily that induces apoptosis in a broad range of human cancer cell lines while sparing most normal cell types. However, many tumors remain resistant to treatment with TRAIL. In this study, we investigated the synergistic effects of low-dose irinotecan (CPT-11) and TRAIL on TRAIL‑resistant HT-29 colon carcinoma cells and explored potential mechanisms of apoptosis. Cell viability was analyzed by sulforhodamine B (SRB) assay and apoptosis was evaluated by flow cytometry and DNA ladder assay. The mRNA expression of TRAIL receptors death receptor 4 (DR4) and DR5 were determined by reverse transcription polymerase chain reaction (RT-PCR). The changes of Bax and caspase-9 in protein levels were also detected by western blotting. Tumor growth curves were depicted and tumor inhibitive rates were calculated. Our results showed that the antitumor effect of TRAIL could be enhanced significantly by low-dose CPT-11 on TRAIL-resistant HT-29 cells both in vitro and in vivo. The synergistic apoptotic effect of CPT-11 and TRAIL was proposed to be mediated by upregulating DR5 mRNA expression and increasing expression of Bax and caspase-9 proteins. The data suggest that the combination of TRAIL with low-dose CPT-11 could be an effective therapeutic approach for HT-29 colon carcinoma.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu H, Zhao F, Yu S, He J, Deng L, Yi C and Huang Y: The synergistic effects of low-dose irinotecan and TRAIL on TRAIL-resistant HT-29 colon carcinoma in vitro and in vivo. Int J Mol Med 30: 1087-1094, 2012.
APA
Zhu, H., Zhao, F., Yu, S., He, J., Deng, L., Yi, C., & Huang, Y. (2012). The synergistic effects of low-dose irinotecan and TRAIL on TRAIL-resistant HT-29 colon carcinoma in vitro and in vivo. International Journal of Molecular Medicine, 30, 1087-1094. https://doi.org/10.3892/ijmm.2012.1105
MLA
Zhu, H., Zhao, F., Yu, S., He, J., Deng, L., Yi, C., Huang, Y."The synergistic effects of low-dose irinotecan and TRAIL on TRAIL-resistant HT-29 colon carcinoma in vitro and in vivo". International Journal of Molecular Medicine 30.5 (2012): 1087-1094.
Chicago
Zhu, H., Zhao, F., Yu, S., He, J., Deng, L., Yi, C., Huang, Y."The synergistic effects of low-dose irinotecan and TRAIL on TRAIL-resistant HT-29 colon carcinoma in vitro and in vivo". International Journal of Molecular Medicine 30, no. 5 (2012): 1087-1094. https://doi.org/10.3892/ijmm.2012.1105
Copy and paste a formatted citation
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Spandidos Publications style
Zhu H, Zhao F, Yu S, He J, Deng L, Yi C and Huang Y: The synergistic effects of low-dose irinotecan and TRAIL on TRAIL-resistant HT-29 colon carcinoma in vitro and in vivo. Int J Mol Med 30: 1087-1094, 2012.
APA
Zhu, H., Zhao, F., Yu, S., He, J., Deng, L., Yi, C., & Huang, Y. (2012). The synergistic effects of low-dose irinotecan and TRAIL on TRAIL-resistant HT-29 colon carcinoma in vitro and in vivo. International Journal of Molecular Medicine, 30, 1087-1094. https://doi.org/10.3892/ijmm.2012.1105
MLA
Zhu, H., Zhao, F., Yu, S., He, J., Deng, L., Yi, C., Huang, Y."The synergistic effects of low-dose irinotecan and TRAIL on TRAIL-resistant HT-29 colon carcinoma in vitro and in vivo". International Journal of Molecular Medicine 30.5 (2012): 1087-1094.
Chicago
Zhu, H., Zhao, F., Yu, S., He, J., Deng, L., Yi, C., Huang, Y."The synergistic effects of low-dose irinotecan and TRAIL on TRAIL-resistant HT-29 colon carcinoma in vitro and in vivo". International Journal of Molecular Medicine 30, no. 5 (2012): 1087-1094. https://doi.org/10.3892/ijmm.2012.1105
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