PDGF-AA and bFGF mediate B104CM-induced proliferation of oligodendrocyte precursor cells

Hu,Jian-Guo; Wu,Xing-Jun; Feng,Yi-Fan; Xi,Gang-Ming; Wang,Zhen-Huan; Zhou,Jian-Sheng; Lü,He-Zuo

doi:10.3892/ijmm.2012.1110

November 2012 Volume 30 Issue 5

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November 2012 Volume 30 Issue 5

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Article

PDGF-AA and bFGF mediate B104CM-induced proliferation of oligodendrocyte precursor cells

Authors:
- Jian-Guo Hu
- Xing-Jun Wu
- Yi-Fan Feng
- Gang-Ming Xi
- Zhen-Huan Wang
- Jian-Sheng Zhou
- He-Zuo Lü
View Affiliations / Copyright

Affiliations: Department of Clinical Laboratory Science, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, P.R. China, Department of Neurology, Xuhui Central Hospital, Shanghai 200031, P.R. China, Department of Clinical Laboratory Science, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, P.R. China, Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, Bengbu, Anhui 233004, P.R. China, Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, Bengbu, Anhui 233004, P.R. China
Pages: 1113-1118
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Published online on: August 24, 2012

https://doi.org/10.3892/ijmm.2012.1110
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Abstract

The conditioned medium from B104 neuroblastoma cells (B104CM) induces proliferation of οligodendrocyte precursor cells (OPCs) in vitro, which indicates that certain factors contained within B104CM give instructional signals that direct the proliferation of OPCs. However, the OPC-proliferative factors present in B104CM have yet to be identified. Platelet-derived growth factor AA (PDGF-AA), basic fibroblast growth factor (bFGF) and insulin-like growth factor-1 (IGF-1) have been reported to act as potent mitogens for OPC proliferation. This raises the possibility that B104CM induces proliferation of OPCs through secretion of PDGF‑AA, bFGF and/or IGF-1. In the present study, we detected the expression and levels of PDGF-AA, bFGF and IGF-1 in B104 cells and B104CM, and observed the expression of their receptors in OPCs. The results indicated that these growth factors were expressed in B104 cells and B104CM. All 3 receptors, PDGFR, FGFR2 and IGF-1R, were also detected in OPCs. Furthermore, B104CM-stimulated OPC proliferation could be markedly decreased by both AG1295 (an inhibitor of PDGFR) and PD173074 (an inhibitor of FGFR). However, the inhibition of IGF-1R with AG1204 did not affect the proliferation of OPCs. Our study suggests that the PDGF-AA and bFGF in B104CM are 2 key factors that stimulate OPC proliferation.

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1.	NP PringleWP YuS GuthrieDetermination of neuroepithelial cell fate: induction of the oligodendrocyte lineage by ventral midline cells and sonic hedgehogDev Biol1773042199610.1006/dbio.1996.01428660874
2.	MC RaffRH MillerM NobleA glial progenitor cell that develops in vitro into an astrocyte or an oligodendrocyte depending on culture mediumNature303390396198310.1038/303390a06304520
3.	JC LouisE MagalD MuirCG-4, a new bipotential glial cell line from rat brain, is capable of differentiating in vitro into either mature oligodendrocytes or type-2 astrocytesJ Neurosci Res31193204199210.1002/jnr.4903101251613821
4.	M NobleSC BarnettO BoglerControl of division and differentiation in oligodendrocyte-type-2 astrocyte progenitor cellsCiba Found Symp15022724319902373025
5.	DG TangYM TokumotoMC RaffLong-term culture of purified postnatal oligodendrocyte precursor cells. Evidence for an intrinsic maturation program that plays out over monthsJ Cell Biol148971984200010.1083/jcb.148.5.971
6.	QL CuiG AlmazanIGF-I-induced oligodendrocyte progenitor proliferation requires PI3K/Akt, MEK/ERK, and Src-like tyrosine kinasesJ Neurochem100148014932007
7.	M Mayer-ProschelAJ KalyaniT MujtabaMS RaoIsolation of lineage-restricted neuronal precursors from multipotent neuroepithelial stem cellsNeuron19773785199710.1016/S0896-6273(00)80960-59354325
8.	T MujtabaDR PiperA KalyaniLineage-restricted neural precursors can be isolated from both the mouse neural tube and cultured ES cellsDev Biol214113127199910.1006/dbio.1999.941810491261
9.	Q CaoXM XuWH DevriesFunctional recovery in traumatic spinal cord injury after transplantation of multineurotrophin-expressing glial-restricted precursor cellsJ Neurosci2569476957200510.1523/JNEUROSCI.1065-05.2005
10.	J HuL DengX WangXM XuEffects of extracellular matrix molecules on the growth properties of oligodendrocyte progenitor cells in vitroJ Neurosci Res8728542862200910.1002/jnr.2211119472225
11.	JG HuSL FuKH ZhangDifferential gene expression in neural stem cells and oligodendrocyte precursor cells: a cDNA microarray analysisJ Neurosci Res78637646200410.1002/jnr.2031715499592
12.	K AsakuraSF HunterM RodriguezEffects of transforming growth factor-beta and platelet-derived growth factor on oligodendrocyte precursors: insights gained from a neuronal cell lineJ Neurochem6822812290199710.1046/j.1471-4159.1997.68062281.x9166720
13.	U McDermottRY AmesAJ IafrateLigand-dependent platelet-derived growth factor receptor (PDGFR)-alpha activation sensitizes rare lung cancer and sarcoma cells to PDGFR kinase inhibitorsCancer Res6939373946200910.1158/0008-5472.CAN-08-4327
14.	S BanaiY WolfG GolombPDGF-receptor tyrosine kinase blocker AG1295 selectively attenuates smooth muscle cell growth in vitro and reduces neointimal formation after balloon angioplasty in swineCirculation9719601969199810.1161/01.CIR.97.19.1960
15.	JG HuYX WangHJ WangMS BaoZh WangX GeFC WangJS ZhouHZ LüPDGF-AA mediates B104CM-induced oligodendrocyte precursor cell differentiation of embryonic neural stem cells through Erk, PI3K, and p38 signalingJ Mol Neurosci46644653201121953009
16.	H HeA LevitzkiHJ ZhuPlatelet-derived growth factor requires epidermal growth factor receptor to activate p21-activated kinase family kinasesJ Biol Chem2762674126744200110.1074/jbc.C10022920011356824
17.	OE PardoJ LatigoRE JefferyThe fibroblast growth factor receptor inhibitor PD173074 blocks small cell lung cancer growth in vitro and in vivoCancer Res6986458651200910.1158/0008-5472.CAN-09-157619903855
18.	F LukY YuWR WalshJL YangIGF1R-targeted therapy and its enhancement of doxorubicin chemosensitivity in human osteosarcoma cell linesCancer Invest29521532201110.3109/07357907.2011.60625221843050

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

PDGF-AA and bFGF mediate B104CM-induced proliferation of oligodendrocyte precursor cells

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