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Article

Titrated extract of Centella asiatica provides a UVB protective effect by altering microRNA expression profiles in human dermal fibroblasts

  • Authors:
    • In-Sook An
    • Sungkwan An
    • Sang-Mo Kang
    • Tae-Boo Choe
    • Sung Nae Lee
    • Hyun Hee Jang
    • Seunghee Bae
  • View Affiliations / Copyright

    Affiliations: Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143-701, Republic of Korea, Department of Microbial Engineering, Konkuk University, Seoul 143-701, Republic of Korea, Department of Cosmetology, Kyung-In Women's College, Incheon Metropolitan City 407-740, Republic of Korea, School of Cosmetology, Kyungbok University, Pocheon-si, Gyeonggi-do 487-717, Republic of Korea, Molecular-Targeted Drug Research Center, Konkuk University, Seoul 143-701, Republic of Korea
  • Pages: 1194-1202
    |
    Published online on: September 4, 2012
       https://doi.org/10.3892/ijmm.2012.1117
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Abstract

The titrated extract of Centella asiatica (TECA) is a reconstituted mixture comprising of asiatic acid, madecassic acid, asiaticoside and madecassoside, and is used as a therapeutic agent in wound healing and also as an anti-microbial, anticancer and anti-aging agent. Although these properties and the associated cell signaling pathways have been elucidated, the cellular mechanism of anti-photoaging upon ultraviolet (UV) exposure in normal human dermal fibroblasts (NHDFs) remains unknown. In this study, we investigated the photoprotective role of TECA via microRNA (miRNA) expression profiling analysis. Low dose of TECA did not exhibit toxicity and showed a protective effect against UVB irradiation in NDHFs. miRNA microarray experiments revealed that specific miRNAs were altered by TECA stimulation in UVB-irradiated NHDFs. Functional bioinformatic analysis showed that the putative target genes of the altered miRNAs were associated with the positive regulation of cell proliferation, anti-apoptosis, small GTPase- and Ras-mediated signal transduction and activation of MAPKK. Therefore, these results suggest that TECA may serve as a potential natural chemoprotective agent against UVB-mediated damage in NHDFs through changes in the expression of specific miRNAs.
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Copy and paste a formatted citation
Spandidos Publications style
An I, An S, Kang S, Choe T, Lee SN, Jang HH and Bae S: Titrated extract of Centella asiatica provides a UVB protective effect by altering microRNA expression profiles in human dermal fibroblasts. Int J Mol Med 30: 1194-1202, 2012.
APA
An, I., An, S., Kang, S., Choe, T., Lee, S.N., Jang, H.H., & Bae, S. (2012). Titrated extract of Centella asiatica provides a UVB protective effect by altering microRNA expression profiles in human dermal fibroblasts. International Journal of Molecular Medicine, 30, 1194-1202. https://doi.org/10.3892/ijmm.2012.1117
MLA
An, I., An, S., Kang, S., Choe, T., Lee, S. N., Jang, H. H., Bae, S."Titrated extract of Centella asiatica provides a UVB protective effect by altering microRNA expression profiles in human dermal fibroblasts". International Journal of Molecular Medicine 30.5 (2012): 1194-1202.
Chicago
An, I., An, S., Kang, S., Choe, T., Lee, S. N., Jang, H. H., Bae, S."Titrated extract of Centella asiatica provides a UVB protective effect by altering microRNA expression profiles in human dermal fibroblasts". International Journal of Molecular Medicine 30, no. 5 (2012): 1194-1202. https://doi.org/10.3892/ijmm.2012.1117
Copy and paste a formatted citation
x
Spandidos Publications style
An I, An S, Kang S, Choe T, Lee SN, Jang HH and Bae S: Titrated extract of Centella asiatica provides a UVB protective effect by altering microRNA expression profiles in human dermal fibroblasts. Int J Mol Med 30: 1194-1202, 2012.
APA
An, I., An, S., Kang, S., Choe, T., Lee, S.N., Jang, H.H., & Bae, S. (2012). Titrated extract of Centella asiatica provides a UVB protective effect by altering microRNA expression profiles in human dermal fibroblasts. International Journal of Molecular Medicine, 30, 1194-1202. https://doi.org/10.3892/ijmm.2012.1117
MLA
An, I., An, S., Kang, S., Choe, T., Lee, S. N., Jang, H. H., Bae, S."Titrated extract of Centella asiatica provides a UVB protective effect by altering microRNA expression profiles in human dermal fibroblasts". International Journal of Molecular Medicine 30.5 (2012): 1194-1202.
Chicago
An, I., An, S., Kang, S., Choe, T., Lee, S. N., Jang, H. H., Bae, S."Titrated extract of Centella asiatica provides a UVB protective effect by altering microRNA expression profiles in human dermal fibroblasts". International Journal of Molecular Medicine 30, no. 5 (2012): 1194-1202. https://doi.org/10.3892/ijmm.2012.1117
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