Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
November 2012 Volume 30 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November 2012 Volume 30 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Silk fibroin hydrolysate inhibits osteoclastogenesis and induces apoptosis of osteoclasts derived from RAW 264.7 cells

  • Authors:
    • Jeon-Woo Chon
    • Hyeryeon Kim
    • Ha-Na Jeon
    • Kyungho Park
    • Kwang-Gill Lee
    • Joo-Hong Yeo
    • Haeyong Kweon
    • Heui-Sam Lee
    • You-Young Jo
    • Yoo Kyoung Park
  • View Affiliations / Copyright

    Affiliations: Sericultural and Apicultural Materials Division, National Academy of Agricultural Science, RDA, Suwon 441-100, Republic of Korea, Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University, Yong-in 446-701, Republic of Korea
  • Pages: 1203-1210
    |
    Published online on: September 6, 2012
       https://doi.org/10.3892/ijmm.2012.1120
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Bone disease can be associated with bone resorption by osteoclasts, and interest in the development of antiresorptive agents has recently increased. The hydrolysate of silk fibroin has been studied with respect to such biomedical applications. In a previous study, silk fibroin showed indirect inhibitory effects on the differentiation of osteoclasts. To further evaluate the effect of a hydrolysate of silk fibroin on osteoclasts, we investigated the direct effects of the silk fibroin hydrolysate on osteoclastogenesis and apoptosis of osteoclasts induced by receptor activation of nuclear factor κB ligand (RANKL). The silk fibroin hydrolysate inhibited RANKL-induced formation of tartrate-resistant acid phosphatase (TRAP) in RAW 264.7 cells. The inhibitory effect of the silk fibroin hydrolysate resulted in the decreased expression of osteoclast marker genes, such as matrix metalloproteinase-9 (MMP-9), cathepsin-K and calcitonin receptor (CTR). In addition, the silk fibroin hydrolysate blocked the signaling pathways of mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) and expression of transcription factors, such as nuclear factor of activated T cells c1 (NFATc1) and NF-κB. Finally, the silk fibroin hydrolysate induced apoptosis signaling cascades. Taken together, the present results indicate that silk fibroin hydrolysate has antiresorptive activity by both inhibiting osteoclastogenesis and inducing osteoclast apoptosis.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1. 

AM ParfittBone remodeling and bone loss: Understanding the pathophysiology of osteoporosisClin Obstet Gynecol30789811198710.1097/00003081-198712000-000043319313

2. 

WJ BoyleWS SimonetDL LaceyOsteoclast differentiation and activationNature423337342200310.1038/nature0165812748652

3. 

K MatsuoN IrieOsteoclast-osteoblast communicationArch Biochem Biophys473201209200810.1016/j.abb.2008.03.027

4. 

BG DarnayBB AggarwalSignal transduction by tumour necrosis factor and tumour necrosis factor related ligands and their receptorsAnn Rheum Dis5812113199910.1136/ard.58.2008.i210577967

5. 

T KogaM InuiK InoueSH KimA SuematsuE KobayashiT IwataH OhnishiT MatozakiT KodamaCostimulatory signals mediated by the ITAM motif cooperate with RANKL for bone homeostasisNature428758763200410.1038/nature0244415085135

6. 

O AnusaksathienC LaplaceX LiY RenL PengSR GoldringDL GalsonTissue-specific and ubiquitous promoters direct the expression of alternatively spliced transcripts from the calcitonin receptor geneJ Biol Chem2762266322674200110.1074/jbc.M00710420011309373

7. 

BF BoyceK WrightSV ReddyBA KoopB StoryR DevlinRJ LeachGD RoodmanJJ WindleTargeting simian virus 40 T antigen to the osteoclast in transgenic mice causes osteoclast tumors and transformation and apoptosis of osteoclastsEndocrinology1365751575919957588333

8. 

DE HughesBF BoyceApoptosis in bone physiology and diseaseMol Pathol50132137199710.1136/mp.50.3.1329292147

9. 

X WuMA McKennaX FengTR NagyJM McDonaldOsteoclast apoptosis: the role of fas in vivo and in vitroEndocrinology1445544555552003

10. 

L XingBF BoyceRegulation of apoptosis in osteoclasts and osteoblastic cellsBiochem Biophys Res Commun328709720200510.1016/j.bbrc.2004.11.07215694405

11. 

GA RodanTJ MartinTherapeutic approaches to bone diseasesScience28915081514200010.1126/science.289.5484.150810968781

12. 

T YamashitaZ YaoF LiQ ZhangIR BadellEM SchwarzS TakeshitaEF WagnerM NodaK MatsuoNF-kappaB p50 and p52 regulate receptor activator of NF-kappaB ligand (RANKL) and tumor necrosis factor-induced osteoclast precursor differentiation by activating c-fos and NFATc1J Biol Chem2821824518253200710.1074/jbc.M610701200

13. 

KH ParkWC JuYH ChoConditioned medium of soybean extract treated osteoblasts inhibits RANKL induced differentiation of osteoclastsFASEB J3964702010

14. 

JM ShinCK ParkEJ ShinTH ChoIK HwangEffects of Scutellaria radix extract on osteoblast differentiation and osteoclast formationKorean J Food Sci Tech406746792008

15. 

G WangH YangM LiS LuX ChenX CaiThe use of silk fibroin/hydroxyapatite composite co-cultured with rabbit bone-marrow stromal cells in the healing of a segmental bone defectJ Bone Joint Surg Br92320325201010.1302/0301-620X.92B2.2260220130332

16. 

C ZhouF ConfalonieriM JacquetR PerassoZ LiJ JaninSilk fibroin: structural implications of a remarkable amino acid sequenceProteins44119122200110.1002/prot.107811391774

17. 

ED KimT BayaraaEJ ShinCK HyunFibroin-derived peptides stimulate glucose transport in normal and insulin-resistant 3T3-L1 adipocytesBiol Pharm Bull32427433200910.1248/bpb.32.42719252290

18. 

M TsukadaG FreddiN MinouraG AllaraPreparation and application of porous silk fibroin materialsJ Appl Polym Sci54507514199410.1002/app.1994.070540411

19. 

A NishidaM YamadaT KanazawaY TakashimaK OuchiH OkadaUse of silk protein, sericin, as a sustained-release material in the form of a gel, sponge and filmChem Pharm Bull5814801486201010.1248/cpb.58.148021048340

20. 

N KatoS SatoA YamanakaH YamadaN FuwaM NomuraSilk protein, sericin, inhibits lipid peroxidation and tyrosinase activityBiosci Biotechnol Biochem62145147199810.1271/bbb.62.1459501526

21. 

C AcharyaSK GhoshSC KunduSilk fibroin protein from mulberry and non-mulberry silkworms: cytotoxicity, biocompatibility and kinetics of L929 murine fibroblast adhesionJ Mater Sci Mater Med1928272836200810.1007/s10856-008-3408-318322779

22. 

N MinouraS AibaY GotohM TsukadaY ImaiAttachment and growth of cultured fibroblast cells on silk protein matricesJ Biomed Mater Res2912151221199510.1002/jbm.8202910088557723

23. 

S SofiaMB McCarthyG GronowizaDL KaplanFunctionalized silk-based biomaterials for bone formationJ Biomed Mater Res54139148200110.1002/1097-4636(200101)54:1%3C139::AID-JBM17%3E3.0.CO;2-711077413

24. 

DH RohSY KangJY KimYB KwonH Young KweonKG LeeYH ParkRM BaekCY HeoJ ChoeWound healing effect of silk fibroin/alginate-blended sponge in full thickness skin defect of ratJ Biomed Mater Res17547552200616691353

25. 

T HanawaA WatanabeT TsuchiyaR IkomaM HidakaM SugiharaNew oral dosage form for elderly patients. II. Release behavior of benfotiamine from silk fibroin gelChem Pharm Bull (Tokyo)43872876199510.1248/cpb.43.8727553973

26. 

LG GriffithG NaughtonTissue engineering - current challenges and expanding opportunitiesScience29510091014200210.1126/science.106921011834815

27. 

JH YeoKH ParkWC JuJA LeeGG LeeSO WooSM HanHY KweonSS KimYH ChoInhibitory effect of conditioned medium of silk fibroin-treated osteoblasts in osteoclast differentiationJ Korean Soc Food Sci Nutr37992997200810.3746/jkfn.2008.37.8.992

28. 

MM BradfordA rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnal Biochem72248254197610.1016/0003-2697(76)90527-3942051

29. 

K MatsuzakiN UdagawaN TakahashiK YamaguchiH YasudaN ShimaT MorinagaY ToyamaY YabeK HigashioOsteoclast differentiation factor (ODF) induces osteoclast-like cell formation in human peripheral blood mononuclear cell culturesBiochem Biophys Res Commun246199204199810.1006/bbrc.1998.85869600092

30. 

E JimiS AkiyamaT TsurukaiN OkahashiK KobayashiN UdagawaT NishiharaN TakahashiT SudaOsteoclast differentiation factor acts as a multifunctional regulator in murine osteoclast differentiation and functionJ Immunol163434442199910384146

31. 

SE LeeKM WooSY KimH KimK KwackZH LeeH KimThe phosphatidylinositol 3-kinase, p38, and extracellular signal-regulated kinase pathways are involved in osteoclast differentiationBone307177200210.1016/S8756-3282(01)00657-311792567

32. 

GH AltmanF DiazC JakubaT CalabroRL HoranJ ChenH LuJ RichmondDL KaplanSilk-based biomaterialsBiomaterials24401416200310.1016/S0142-9612(02)00353-812423595

33. 

P ReponenC SahlbergC MunautI ThesleffK TryggvasonHigh expression of 92-kD type IV collagenase (gelatinase B) in the osteoclast lineage during mouse developmentJ Cell Biol12410911102199410.1083/jcb.124.6.10918132709

34. 

BD GelbGP ShiHA ChapmanRJ DesnickPycnodysostosis, a lysosomal disease caused by cathepsin K deficiencyScience27912361238199610.1126/science.273.5279.12368703060

35. 

T IshikawaM KamiyamaN Tani-IshiiH SuzukiY IchikawaY HamaguchiN MomiyamaH ShimadaInhibition of osteoclast differentiation and bone resorption by cathepsin K antisense oligonucleotidesMol Carcinog328491200110.1002/mc.106711746820

36. 

K KanazawaA KudoTRAF2 is essential for TNF-alpha-induced osteoclastogenesisJ Bone Miner Res20840847200510.1359/JBMR.04122515824857

37. 

ZH LeeH KimSignal transduction by receptor activator of nuclear factor kappa B in osteoclastsBiochem Biophys Res Commun305211214200310.1016/S0006-291X(03)00695-812745060

38. 

H TakayanagiS KimT KogaH NishinaM IsshikiH YoshidaA SaiuraM IsobeT YokochiJ InoueInduction and activation of the transcription factor NFATc1 (NFAT2) integrate RANKL signaling in terminal differentiation of osteoclastsDev Cell3889901200210.1016/S1534-5807(02)00369-612479813

39. 

G KarsentyEF WagnerReaching a genetic and molecular understanding of skeletal developmentDev Cell2389406200210.1016/S1534-5807(02)00157-011970890

40. 

N IshidaK HayashiM HoshijimaT OgawaS KogaY MiyatakeM KumegawaT KimuraT TakeyaLarge scale gene expression analysis of osteoclastogenesis in vitro and elucidation of NFAT2 as a key regulatorJ Biol Chem2774114741156200210.1074/jbc.M20506320012171919

41. 

K MatsuoDL GalsonC ZhaoL PengC LaplaceKZ WangMA BachlerH AmanoH AburataniH IshikawaNuclear factor of activated T-cells (NFAT) rescues osteoclastogenesis in precursors lacking c-fosJ Biol Chem2792647526480200410.1074/jbc.M31397320015073183

42. 

PH SternAntiresorptive agents and osteoclast apoptosisJ Cell Biochem10110871096200710.1002/jcb.2131117407157

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Chon J, Kim H, Jeon H, Park K, Lee K, Yeo J, Kweon H, Lee H, Jo Y, Park YK, Park YK, et al: Silk fibroin hydrolysate inhibits osteoclastogenesis and induces apoptosis of osteoclasts derived from RAW 264.7 cells. Int J Mol Med 30: 1203-1210, 2012.
APA
Chon, J., Kim, H., Jeon, H., Park, K., Lee, K., Yeo, J. ... Park, Y.K. (2012). Silk fibroin hydrolysate inhibits osteoclastogenesis and induces apoptosis of osteoclasts derived from RAW 264.7 cells. International Journal of Molecular Medicine, 30, 1203-1210. https://doi.org/10.3892/ijmm.2012.1120
MLA
Chon, J., Kim, H., Jeon, H., Park, K., Lee, K., Yeo, J., Kweon, H., Lee, H., Jo, Y., Park, Y. K."Silk fibroin hydrolysate inhibits osteoclastogenesis and induces apoptosis of osteoclasts derived from RAW 264.7 cells". International Journal of Molecular Medicine 30.5 (2012): 1203-1210.
Chicago
Chon, J., Kim, H., Jeon, H., Park, K., Lee, K., Yeo, J., Kweon, H., Lee, H., Jo, Y., Park, Y. K."Silk fibroin hydrolysate inhibits osteoclastogenesis and induces apoptosis of osteoclasts derived from RAW 264.7 cells". International Journal of Molecular Medicine 30, no. 5 (2012): 1203-1210. https://doi.org/10.3892/ijmm.2012.1120
Copy and paste a formatted citation
x
Spandidos Publications style
Chon J, Kim H, Jeon H, Park K, Lee K, Yeo J, Kweon H, Lee H, Jo Y, Park YK, Park YK, et al: Silk fibroin hydrolysate inhibits osteoclastogenesis and induces apoptosis of osteoclasts derived from RAW 264.7 cells. Int J Mol Med 30: 1203-1210, 2012.
APA
Chon, J., Kim, H., Jeon, H., Park, K., Lee, K., Yeo, J. ... Park, Y.K. (2012). Silk fibroin hydrolysate inhibits osteoclastogenesis and induces apoptosis of osteoclasts derived from RAW 264.7 cells. International Journal of Molecular Medicine, 30, 1203-1210. https://doi.org/10.3892/ijmm.2012.1120
MLA
Chon, J., Kim, H., Jeon, H., Park, K., Lee, K., Yeo, J., Kweon, H., Lee, H., Jo, Y., Park, Y. K."Silk fibroin hydrolysate inhibits osteoclastogenesis and induces apoptosis of osteoclasts derived from RAW 264.7 cells". International Journal of Molecular Medicine 30.5 (2012): 1203-1210.
Chicago
Chon, J., Kim, H., Jeon, H., Park, K., Lee, K., Yeo, J., Kweon, H., Lee, H., Jo, Y., Park, Y. K."Silk fibroin hydrolysate inhibits osteoclastogenesis and induces apoptosis of osteoclasts derived from RAW 264.7 cells". International Journal of Molecular Medicine 30, no. 5 (2012): 1203-1210. https://doi.org/10.3892/ijmm.2012.1120
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team