Silk fibroin hydrolysate inhibits osteoclastogenesis and induces apoptosis of osteoclasts derived from RAW 264.7 cells

  • Authors:
    • Jeon-Woo Chon
    • Hyeryeon Kim
    • Ha-Na Jeon
    • Kyungho Park
    • Kwang-Gill Lee
    • Joo-Hong Yeo
    • Haeyong Kweon
    • Heui-Sam Lee
    • You-Young Jo
    • Yoo Kyoung Park
  • View Affiliations

  • Published online on: September 6, 2012     https://doi.org/10.3892/ijmm.2012.1120
  • Pages: 1203-1210
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Abstract

Bone disease can be associated with bone resorption by osteoclasts, and interest in the development of antiresorptive agents has recently increased. The hydrolysate of silk fibroin has been studied with respect to such biomedical applications. In a previous study, silk fibroin showed indirect inhibitory effects on the differentiation of osteoclasts. To further evaluate the effect of a hydrolysate of silk fibroin on osteoclasts, we investigated the direct effects of the silk fibroin hydrolysate on osteoclastogenesis and apoptosis of osteoclasts induced by receptor activation of nuclear factor κB ligand (RANKL). The silk fibroin hydrolysate inhibited RANKL-induced formation of tartrate-resistant acid phosphatase (TRAP) in RAW 264.7 cells. The inhibitory effect of the silk fibroin hydrolysate resulted in the decreased expression of osteoclast marker genes, such as matrix metalloproteinase-9 (MMP-9), cathepsin-K and calcitonin receptor (CTR). In addition, the silk fibroin hydrolysate blocked the signaling pathways of mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) and expression of transcription factors, such as nuclear factor of activated T cells c1 (NFATc1) and NF-κB. Finally, the silk fibroin hydrolysate induced apoptosis signaling cascades. Taken together, the present results indicate that silk fibroin hydrolysate has antiresorptive activity by both inhibiting osteoclastogenesis and inducing osteoclast apoptosis.
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November 2012
Volume 30 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Chon J, Kim H, Jeon H, Park K, Lee K, Yeo J, Kweon H, Lee H, Jo Y, Park YK, Park YK, et al: Silk fibroin hydrolysate inhibits osteoclastogenesis and induces apoptosis of osteoclasts derived from RAW 264.7 cells. Int J Mol Med 30: 1203-1210, 2012
APA
Chon, J., Kim, H., Jeon, H., Park, K., Lee, K., Yeo, J. ... Park, Y.K. (2012). Silk fibroin hydrolysate inhibits osteoclastogenesis and induces apoptosis of osteoclasts derived from RAW 264.7 cells. International Journal of Molecular Medicine, 30, 1203-1210. https://doi.org/10.3892/ijmm.2012.1120
MLA
Chon, J., Kim, H., Jeon, H., Park, K., Lee, K., Yeo, J., Kweon, H., Lee, H., Jo, Y., Park, Y. K."Silk fibroin hydrolysate inhibits osteoclastogenesis and induces apoptosis of osteoclasts derived from RAW 264.7 cells". International Journal of Molecular Medicine 30.5 (2012): 1203-1210.
Chicago
Chon, J., Kim, H., Jeon, H., Park, K., Lee, K., Yeo, J., Kweon, H., Lee, H., Jo, Y., Park, Y. K."Silk fibroin hydrolysate inhibits osteoclastogenesis and induces apoptosis of osteoclasts derived from RAW 264.7 cells". International Journal of Molecular Medicine 30, no. 5 (2012): 1203-1210. https://doi.org/10.3892/ijmm.2012.1120