Open Access

A novel double antibody sandwich-lateral flow immunoassay for the rapid and simple detection of hepatitis C virus

  • Authors:
    • Tingxiu Xiang
    • Zheng Jiang
    • Jian Zheng
    • Chaoyu Lo
    • Harry Tsou
    • Guosheng Ren
    • Jun Zhang
    • Ailong Huang
    • Guoqi Lai
  • View Affiliations

  • Published online on: September 6, 2012     https://doi.org/10.3892/ijmm.2012.1121
  • Pages: 1041-1047
  • Copyright: © Xiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

The objective of this study was to screen for antigens of the hepatitis C virus (HCV) to establish a new double antibody sandwich-lateral flow immunoassay (DAS-LFIA) method for testing the presence of anti-HCV antibodies in human serum or plasma. A series of different recombinant HCV proteins in Escherichia coli cells were constructed, expressed, purified and the new DAS-LFIA strip was developed. The sensitivity and specificity of new the DAS-LFIA strip were evaluated by detecting 23 HCV-positive sera, a set of quality control references for anti-HCV detection that contain known amounts of anti-HCV antibodies, and 8 HCV-negative sera. A total of 300 clinical serum samples was examined by both the new DAS-LFIA strip and enzyme-linked immunosorbent assay (ELISA). Data were analyzed using SPSS 11.5 software. The sensitivity and specificity of the new DAS-LFIA strip were 100%. The lowest test line of the HCV DAS-LFIA strips was 2 NCU/ml. Additionally, the concordance between the new DAS-LFIA strip and ELISA methods was 94.33%. In conclusion, our new testing method is rapid, simple, sensitive and specifically detects the presence of anti-HCV antibodies in human serum or plasma. Therefore, it may be used for monitoring HCV.
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November 2012
Volume 30 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Xiang T, Jiang Z, Zheng J, Lo C, Tsou H, Ren G, Zhang J, Huang A and Lai G: A novel double antibody sandwich-lateral flow immunoassay for the rapid and simple detection of hepatitis C virus. Int J Mol Med 30: 1041-1047, 2012.
APA
Xiang, T., Jiang, Z., Zheng, J., Lo, C., Tsou, H., Ren, G. ... Lai, G. (2012). A novel double antibody sandwich-lateral flow immunoassay for the rapid and simple detection of hepatitis C virus. International Journal of Molecular Medicine, 30, 1041-1047. https://doi.org/10.3892/ijmm.2012.1121
MLA
Xiang, T., Jiang, Z., Zheng, J., Lo, C., Tsou, H., Ren, G., Zhang, J., Huang, A., Lai, G."A novel double antibody sandwich-lateral flow immunoassay for the rapid and simple detection of hepatitis C virus". International Journal of Molecular Medicine 30.5 (2012): 1041-1047.
Chicago
Xiang, T., Jiang, Z., Zheng, J., Lo, C., Tsou, H., Ren, G., Zhang, J., Huang, A., Lai, G."A novel double antibody sandwich-lateral flow immunoassay for the rapid and simple detection of hepatitis C virus". International Journal of Molecular Medicine 30, no. 5 (2012): 1041-1047. https://doi.org/10.3892/ijmm.2012.1121