A novel double antibody sandwich-lateral flow immunoassay for the rapid and simple detection of hepatitis C virus

Xiang,Tingxiu; Jiang,Zheng; Zheng,Jian; Lo,Chaoyu; Tsou,Harry; Ren,Guosheng; Zhang,Jun; Huang,Ailong; Lai,Guoqi

doi:10.3892/ijmm.2012.1121

November 2012 Volume 30 Issue 5

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November 2012 Volume 30 Issue 5

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Article Open Access

A novel double antibody sandwich-lateral flow immunoassay for the rapid and simple detection of hepatitis C virus

Authors:
- Tingxiu Xiang
- Zheng Jiang
- Jian Zheng
- Chaoyu Lo
- Harry Tsou
- Guosheng Ren
- Jun Zhang
- Ailong Huang
- Guoqi Lai
View Affiliations / Copyright

Affiliations: Molecular Oncology and Epigenetic Laboratory, The First Affiliated Hospital of Chongqing Medical University, Chongqing, P.R. China, Department of Gastroenterology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, P.R. China, Artron BioResearch Inc., Burnaby, BC, Canada, Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, P.R. China

Copyright: © Xiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
Pages: 1041-1047
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Published online on: September 6, 2012

https://doi.org/10.3892/ijmm.2012.1121
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Abstract

The objective of this study was to screen for antigens of the hepatitis C virus (HCV) to establish a new double antibody sandwich-lateral flow immunoassay (DAS-LFIA) method for testing the presence of anti-HCV antibodies in human serum or plasma. A series of different recombinant HCV proteins in Escherichia coli cells were constructed, expressed, purified and the new DAS-LFIA strip was developed. The sensitivity and specificity of new the DAS-LFIA strip were evaluated by detecting 23 HCV-positive sera, a set of quality control references for anti-HCV detection that contain known amounts of anti-HCV antibodies, and 8 HCV-negative sera. A total of 300 clinical serum samples was examined by both the new DAS-LFIA strip and enzyme-linked immunosorbent assay (ELISA). Data were analyzed using SPSS 11.5 software. The sensitivity and specificity of the new DAS-LFIA strip were 100%. The lowest test line of the HCV DAS-LFIA strips was 2 NCU/ml. Additionally, the concordance between the new DAS-LFIA strip and ELISA methods was 94.33%. In conclusion, our new testing method is rapid, simple, sensitive and specifically detects the presence of anti-HCV antibodies in human serum or plasma. Therefore, it may be used for monitoring HCV.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

A novel double antibody sandwich-lateral flow immunoassay for the rapid and simple detection of hepatitis C virus

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