Regulation of mammalian MOR-1 gene expression after chronic treatment with morphine

Prenus,Rose  V.; Luscar,Ebens; Zhu,Zhi-Ping; Badisa,Ramesh   B.; Goodman,Carl  B.

doi:10.3892/ijmm.2012.1132

December 2012 Volume 30 Issue 6

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December 2012 Volume 30 Issue 6

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Article

Regulation of mammalian MOR-1 gene expression after chronic treatment with morphine

Authors:
- Rose V. Prenus
- Ebens Luscar
- Zhi-Ping Zhu
- Ramesh B. Badisa
- Carl B. Goodman
View Affiliations / Copyright

Affiliations: College of Pharmacy and Pharmaceutical Sciences, Florida A__AMB__M University, Tallahassee, FL 32307, USA
Pages: 1493-1497
|
Published online on: September 19, 2012

https://doi.org/10.3892/ijmm.2012.1132
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Abstract

Morphine is an effective analgesic that acts by binding to the µ-opioid receptor (MOR) coded in the human by the OPRM1 gene. In the present study, we investigated the regulation of µ-opioid receptor (MOR-1) mRNA levels in all-trans-retinoic acid-differentiated SH-SY5Y human neuroblastoma cells under in vitro conditions with 10 µM morphine treatment for 24 h. In addition, we measured the MOR-1 levels in recombinant Chinese hamster ovary (CHO) cells, transfected with human µ-opioid receptor gene (hMOR) with 10 µM morphine treatment for 24 h. The isolated mRNA from these cells was subjected to real-time quantitative RT-PCR analysis to determine the regulation of µ-opioid receptor gene expression. It was observed that morphine treatment did not alter MOR-1 levels in undifferentiated SH-SY5Y cells compared to undifferentiated control cells. However, the MOR-1 levels in all-trans-retinoic acid-differentiated cells were significantly higher compared to the undifferentiated cells. Morphine treatment in differentiated SH-SY5Y cells caused significant downregulation of MOR-1 expression compared to the control cells. In the morphine-treated CHO cells, the hMOR-1 mRNA levels remained the same as the untreated control. Finally, pretreatment of SH-SY5Y cells with 10 µM naloxone, the antagonist of µ-opioid receptor, for 1 h significantly blocked the downregulation of MOR-1 mRNA levels with morphine treatment. These findings suggest that regulation of MOR-1 gene expression is cell-type specific after chronic morphine treatment and provide some evidence in the understanding of morphine tolerance.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Regulation of mammalian MOR-1 gene expression after chronic treatment with morphine

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