Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
December 2012 Volume 30 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December 2012 Volume 30 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Hexane extract from Uncaria sinensis exhibits anti-apoptotic properties against glutamate-induced neurotoxicity in primary cultured cortical neurons

  • Authors:
    • Ji-Yeon Jang
    • Ha-Neui Kim
    • Yu-Ri Kim
    • Jin-Woo Hong
    • Young-Whan Choi
    • Yung-Hyun Choi
    • Hwa-Kyoung Shin
    • Byung-Tae Choi
  • View Affiliations / Copyright

    Affiliations: Division of Meridian and Structural Medicine, Pusan National University, Yangsan 626-870, Republic of Korea, Division of Clinical Medicine I, School of Korean Medicine, Pusan National University, Yangsan 626-870, Republic of Korea, Department of Horticultural Bioscience, College of Natural Resources and Life Science, Pusan National University, Miryang 626-706, Republic of Korea, Department of Biochemistry, College of Oriental Medicine, Dongeui University, Busan 614-052, Republic of Korea
  • Pages: 1465-1472
    |
    Published online on: September 19, 2012
       https://doi.org/10.3892/ijmm.2012.1134
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

We explored the neuroprotective effects of a hexane extract from Uncaria sinensis (HEUS) against glutamate-induced toxicity focusing on its anti-apoptotic mechanism in primary cultured cortical neurons. Pretreatment with HEUS resulted in significantly reduced glutamate-induced toxicity in a dose-dependent manner with a decrease in the release of lactate dehydrogenase. Morphological assay and flow cytometry were performed for determination of the type of cell death; according to the results, treatment with HEUS resulted in a significant reduction of glutamate-induced apoptotic cell death. We examined the anti-apoptotic mechanism of HEUS; treatment with HEUS resulted in markedly decreased expression of death receptor (DR)4, which was induced by glutamate stimulation. In contrast, treatment with HEUS resulted in significantly enhanced levels of expression of glutamate-attenuated XIAP and Bcl-2, as well as marked blockade of glutamate-induced Bid cleavage, which inhibits both extrinsic and intrinsic apoptosis pathways. In addition, pretreatment with HEUS resulted in almost complete blockade of glutamate-induced activation of caspases-8, -9 and -3, as well as cleavage of poly (ADP-ribose) polymerase. These findings suggest that the neuroprotective effects of HEUS against glutamate-induced toxicity occur via inhibition of DR4 and expression of anti-apoptotic proteins XIAP and Bcl-2 resulting in effective abrogation of the activation of caspase cascades and promotion of cell survival.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1. 

BS MeldrumGlutamate as a neurotransmitter in the brain: review of physiology and pathologyJ Nutr130Suppl 41007S1015S200010736372

2. 

JT CoyleP PuttfarckenOxidative stress, glutamate, and neurodegenerative disordersScience262689695199310.1126/science.79019087901908

3. 

Y NishizawaGlutamate release and neuronal damage in ischemiaLife Sci69369381200110.1016/S0024-3205(01)01142-011459428

4. 

AK StoutHM RaphaelBI KanterewiczE KlannIJ ReynoldsGlutamate-induced neuron death requires mitochondrial calcium uptakeNat Neurosci5366373199810196525

5. 

Y ZhangBR BhavnaniGlutamate-induced apoptosis in primary cortical neurons is inhibited by equine estrogens via down-regulation of caspase-3 and prevention of mitochondrial cytochrome c releaseBMC Neurosci24613200515730564

6. 

A AtlanteP CalissanoA BobbaA AzzaritiE MarraS PassarellaCytochrome c is released from mitochondria in a reactive oxygen species (ROS)-dependent fashion and can operate as a ROS scavenger and as a respiratory substrate in cerebellar neurons undergoing excitotoxic deathJ Biol Chem473715937166200010.1074/jbc.M002361200

7. 

W PaschenGlutamate excitotoxicity in transient global cerebral ischemiaActa Neurobiol Exp (Wars)5631332219968787192

8. 

MR HyndHL ScottPR DoddGlutamate-mediated excitotoxicity and neurodegeneration in Alzheimer’s diseaseNeurochem Int455835952004

9. 

PC MayPN GrayThe mechanism of glutamate-induced degeneration of cultured Huntington’s disease and control fibroblastsJ Neurol Sci701011121985

10. 

YW LinCL HsiehOral Uncaria rhynchophylla (UR) reduces kainic acid-induced epileptic seizures and neuronal death accompanied by attenuating glial cell proliferation and S100B proteins in ratsJ Ethnopharmacol1353133202011

11. 

T KuramochiJ ChuT SugaGou-teng (from Uncaria rhynchophylla Miquel)-induced endothelium-dependent and -independent relaxations in the isolated rat aortaLife Sci262061206919948208063

12. 

T ItohY ShimadaK TerasawaEfficacy of Choto-san on vascular dementia and the protective effect of the hooks and stems of Uncaria sinensis on glutamate-induced neuronal deathMech Ageing Dev111155173199910.1016/S0047-6374(99)00062-710656534

13. 

J ZhouS ZhouAntihypertensive and neuroprotective activities of rhynchophylline: the role of rhynchophylline in neurotransmission and ion channel activityJ Ethnopharmacol1321527201010.1016/j.jep.2010.08.04120736055

14. 

K SukSY KimK LeemYO KimSY ParkJ HurJ BaekKJ LeeHZ ZhengH KimNeuroprotection by methanol extract of Uncaria rhynchophylla against global cerebral ischemia in ratsLife Sci7024672480200212173411

15. 

Y ShimadaH GotoT KogureN ShibaharaI SakakibaraH SasakiK TerasawaProtective effect of phenolic compounds isolated from the hooks and stems of Uncaria sinensis on glutamate-induced neuronal deathAm J Chin Med29173180200110.1142/S0192415X0100019811321476

16. 

SH ParkJH KimSJ ParkSS BaeYW ChoiJW HongBT ChoiHK ShinProtective effect of hexane extracts of Uncaria sinensis against photothrombotic ischemic injury in miceJ Ethnopharmacol138774779201122051882

17. 

GJ BrewerJR TorricelliEK EvegePJ PriceOptimized survival of hippocampal neurons in B27-supplemented Neurobasal, a new serum-free medium combinationJ Neurosci Res35567576199310.1002/jnr.4903505138377226

18. 

P NicoteraM AnkarcronaE BonfocoS OrreniusSA LiptonNeuronal necrosis and apoptosis: two distinct events induced by exposure to glutamate or oxidative stressAdv Neurol729510119978993688

19. 

S FuldaKM DebatinExtrinsic versus intrinsic apoptosis pathways in anticancer chemotherapyOncogene2547984811200610.1038/sj.onc.120960816892092

20. 

Y HirashimaM KurimotoK NogamiS EndoM SaitohO OhtaniT NagataA MuraguchiA TakakuCorrelation of glutamate-induced apoptosis with caspase activities in cultured rat cerebral cortical neuronsBrain Res849109118199910.1016/S0006-8993(99)02009-010592292

21. 

A LauM TymianskiGlutamate receptors, neurotoxicity and neurodegenerationPflugers Arch460525542201010.1007/s00424-010-0809-120229265

22. 

Y ShimadaH GotoT ItohI SakakibaraM KuboH SasakiK TerasawaEvaluation of the protective effects of alkaloids isolated from the hooks and stems of Uncaria sinensis on glutamate-induced neuronal death in cultured cerebellar granule cells from ratsJ Pharm Pharmacol51715722199910.1211/002235799177285310454049

23. 

M AnkarcronaJM DypbuktE BonfocoB ZhivotovskyS OrreniusSA LiptonP NicoteraGlutamate-induced neuronal death: a succession of necrosis or apoptosis depending on mitochondrial functionNeuron15961973199510.1016/0896-6273(95)90186-87576644

24. 

Y HuangN ErdmannH PengY ZhaoJ ZhengThe role of TNF related apoptosis-inducing ligand in neurodegenerative diseasesCell Mol Immunol2113122200516191417

25. 

D UbertiG Ferrari-ToninelliSA BoniniI SarnicoM BenareseM PizziL BenussiR GhidoniG BinettiP SpanoF FacchettiM MemoBlockade of the tumor necrosis factor-related apoptosis inducing ligand death receptor DR5 prevents beta-amyloid neurotoxicityNeuropsychopharmacology4872880200616936710

26. 

J YangX LiuK BhallaCN KimAM IbradoJ CaiTI PengDP JonesX WangPrevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blockedScience27511291132199710.1126/science.275.5303.11299027314

27. 

XY LiJ LiangYB TangJG ZhouYY GuanGinsenoside Rd prevents glutamate-induced apoptosis in rat cortical neuronsClin Exp Pharmacol Physiol37199204201010.1111/j.1440-1681.2009.05286.x19719747

28. 

X WangG ZhuS YangX WangH ChengF WangX LiQ LiPaeonol prevents excitotoxicity in rat pheochromocytoma PC12 cells via downregulation of ERK activation and inhibition of apoptosisPlanta Med7716951701201110.1055/s-0030-127103321509715

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Jang J, Kim H, Kim Y, Hong J, Choi Y, Choi Y, Shin H and Choi B: Hexane extract from Uncaria sinensis exhibits anti-apoptotic properties against glutamate-induced neurotoxicity in primary cultured cortical neurons. Int J Mol Med 30: 1465-1472, 2012.
APA
Jang, J., Kim, H., Kim, Y., Hong, J., Choi, Y., Choi, Y. ... Choi, B. (2012). Hexane extract from Uncaria sinensis exhibits anti-apoptotic properties against glutamate-induced neurotoxicity in primary cultured cortical neurons. International Journal of Molecular Medicine, 30, 1465-1472. https://doi.org/10.3892/ijmm.2012.1134
MLA
Jang, J., Kim, H., Kim, Y., Hong, J., Choi, Y., Choi, Y., Shin, H., Choi, B."Hexane extract from Uncaria sinensis exhibits anti-apoptotic properties against glutamate-induced neurotoxicity in primary cultured cortical neurons". International Journal of Molecular Medicine 30.6 (2012): 1465-1472.
Chicago
Jang, J., Kim, H., Kim, Y., Hong, J., Choi, Y., Choi, Y., Shin, H., Choi, B."Hexane extract from Uncaria sinensis exhibits anti-apoptotic properties against glutamate-induced neurotoxicity in primary cultured cortical neurons". International Journal of Molecular Medicine 30, no. 6 (2012): 1465-1472. https://doi.org/10.3892/ijmm.2012.1134
Copy and paste a formatted citation
x
Spandidos Publications style
Jang J, Kim H, Kim Y, Hong J, Choi Y, Choi Y, Shin H and Choi B: Hexane extract from Uncaria sinensis exhibits anti-apoptotic properties against glutamate-induced neurotoxicity in primary cultured cortical neurons. Int J Mol Med 30: 1465-1472, 2012.
APA
Jang, J., Kim, H., Kim, Y., Hong, J., Choi, Y., Choi, Y. ... Choi, B. (2012). Hexane extract from Uncaria sinensis exhibits anti-apoptotic properties against glutamate-induced neurotoxicity in primary cultured cortical neurons. International Journal of Molecular Medicine, 30, 1465-1472. https://doi.org/10.3892/ijmm.2012.1134
MLA
Jang, J., Kim, H., Kim, Y., Hong, J., Choi, Y., Choi, Y., Shin, H., Choi, B."Hexane extract from Uncaria sinensis exhibits anti-apoptotic properties against glutamate-induced neurotoxicity in primary cultured cortical neurons". International Journal of Molecular Medicine 30.6 (2012): 1465-1472.
Chicago
Jang, J., Kim, H., Kim, Y., Hong, J., Choi, Y., Choi, Y., Shin, H., Choi, B."Hexane extract from Uncaria sinensis exhibits anti-apoptotic properties against glutamate-induced neurotoxicity in primary cultured cortical neurons". International Journal of Molecular Medicine 30, no. 6 (2012): 1465-1472. https://doi.org/10.3892/ijmm.2012.1134
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team