Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
December 2012 Volume 30 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December 2012 Volume 30 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Sphingosine-1-phosphate inhibits interleukin-1β-induced inflammation in human articular chondrocytes

  • Authors:
    • Myung-Hee Moon
    • Jae-Kyo Jeong
    • You-Jin Lee
    • Jae-Won Seol
    • Sang-Youel Park
  • View Affiliations / Copyright

    Affiliations: Biosafety Research Institute, College of Veterinary Medicine, Chonbuk National University, Jeonju, Jeonbuk 561-756, Republic of Korea
  • Pages: 1451-1458
    |
    Published online on: September 19, 2012
       https://doi.org/10.3892/ijmm.2012.1135
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Sphingosine-1-phosphate (S1P) is a pluripotent lipid mediator that transmits signals through a family of G-protein-coupled receptors (GPCRs) to control diverse biological processes including inflammation and wound-healing. In this study, a novel biological activity of S1P in articular chondrocytes was identified. Human primary chondrocytes were cultured in a monolayer. Reverse transcription-polymerase chain reaction (RT-PCR) and western blotting were performed to detect genes and proteins involved in inflammation and cartilage degradation when human primary chondrocytes were stimulated by interleukin (IL)-1β. Matrix metalloproteinase (MMP)-2 and MMP-9 activity was evaluated by gelatin zymography. Glycosaminoglycan (GAG) degradation was evaluated using the dimethylene blue method. Prostaglandin E2 (PGE2) was measured by enzyme-linked immunosorbent assay (ELISA). By using the S1P1 receptor agonist and antagonist, we discovered the key role played by S1P1 in the S1P-dependent inhibition of IL-1β-induced inflammation in human chondrocytes. S1P dose-dependently inhibited IL-1β-induced NF-κB p65, cyclooxygenase (COX)-2, MMP-1, MMP-3, MMP-13 and MMP-14 mRNA expression in human chondrocytes and IL-1β-induced PGE2 synthesis and GAG degradation in human cartilage explants. W146, a known S1P1 receptor antagonist, inhibited the active form of NF-κB p65 and COX-2 expression induced by IL-1β. The anti-inflammatory action of the S1P1 receptor agonist SEW2871 was similar to that of S1P. This study demonstrates that S1P has anti-inflammatory effects on chondrocytes via the S1P1 receptor. Our data suggest that targeting S1P and S1P1 may be a potential therapy for arthritis.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1. 

N ChabaneN ZayedH AfifHistone deacetylase inhibitors suppress interleukin-1beta-induced nitric oxide and prostaglandin E2 production in human chondrocytesOsteoarthritis Cartilage1612671274200810.1016/j.joca.2008.03.00918417374

2. 

MB GoldringKB MarcuCartilage homeostasis in health and rheumatic diseasesArthritis Res Ther11224200910.1186/ar259219519926

3. 

MB GoldringF BerenbaumHuman chondrocyte culture models for studying cyclooxygenase expression and prostaglandin regulation of collagen gene expressionOsteoarthritis Cartilage7386388199910.1053/joca.1998.0219

4. 

HA KimY YeoWU KimS KimPhase II enzyme inducer sulphoraphane blocks matrix metalloproteinase production in articular chondrocytesRheumatology (Oxford)48932938200910.1093/rheumatology/kep132

5. 

C LianxuJ HongtiY ChanglongNF-kappaBp65-specific siRNA inhibits expression of genes of COX-2, NOS-2 and MMP-9 in rat IL-1beta-induced and TNF-alpha-induced chondrocytesOsteoarthritis Cartilage14367376200610.1016/j.joca.2005.10.00916376111

6. 

JP PelletierJ Martel-PelletierSB AbramsonOsteoarthritis, an inflammatory disease: potential implication for the selection of new therapeutic targetsArthritis Rheum4412371247200110.1002/1529-0131(200106)44:6%3C1237::AID-ART214%3E3.0.CO;2-F11407681

7. 

EB KoppS GhoshNF-kappa B and rel proteins in innate immunityAdv Immunol58127199510.1016/S0065-2776(08)60618-57741027

8. 

AS Baldwin JrThe NF-kappa B and I kappa B proteins: new discoveries and insightsAnnu Rev Immunol14649683199610.1146/annurev.immunol.14.1.6498717528

9. 

R NewtonLM KuitertM BergmannIM AdcockPJ BarnesEvidence for involvement of NF-kappaB in the transcriptional control of COX-2 gene expression by IL-1betaBiochem Biophys Res Commun2372832199710.1006/bbrc.1997.70649266823

10. 

M ShakibaeiC CsakiS NebrichA MobasheriResveratrol suppresses interleukin-1beta-induced inflammatory signaling and apoptosis in human articular chondrocytes: potential for use as a novel nutraceutical for the treatment of osteoarthritisBiochem Pharmacol7614261439200810.1016/j.bcp.2008.05.029

11. 

ED JohnstoneG ChanCP SibleyST DavidgeB LowenLJ GuilbertSphingosine-1-phosphate inhibition of placental trophoblast differentiation through a G(i)-coupled receptor responseJ Lipid Res4618331839200510.1194/jlr.M500095-JLR200

12. 

M SchuppelU KurschnerU KleuserM Schafer-KortingB KleuserSphingosine 1-phosphate restrains insulin-mediated keratinocyte proliferation via inhibition of Akt through the S1P2 receptor subtypeJ Invest Dermatol12817471756200810.1038/sj.jid.570125918219276

13. 

S PyneNJ PyneSphingosine 1-phosphate signalling in mammalian cellsBiochem J349385402200010.1042/0264-6021:349038510880336

14. 

MH StradnerJ HermannH AngererSphingosine-1-phosphate stimulates proliferation and counteracts interleukin-1 induced nitric oxide formation in articular chondrocytesOsteoarthritis Cartilage16305311200810.1016/j.joca.2007.06.018

15. 

R OgawaM TakahashiS HiroseA novel sphingosine-1-phosphate receptor agonist KRP-203 attenuates rat autoimmune myocarditisBiochem Biophys Res Commun361621628200710.1016/j.bbrc.2007.07.06117673173

16. 

JE HughesS SrinivasanKR LynchRL ProiaP FerdekCC HedrickSphingosine-1-phosphate induces an antiinflammatory phenotype in macrophagesCirc Res102950958200810.1161/CIRCRESAHA.107.17077918323526

17. 

JW SeolHB LeeNS KimSY ParkTartrate-resistant acid phosphatase as a diagnostic factor for arthritisInt J Mol Med245762200919513535

18. 

MB GoldringThe role of cytokines as inflammatory mediators in osteoarthritis: lessons from animal modelsConnect Tissue Res40111199910.3109/0300820990900527310770646

19. 

CM ThomasCJ FullerCE WhittlesM SharifChondrocyte death by apoptosis is associated with cartilage matrix degradationOsteoarthritis Cartilage152734200710.1016/j.joca.2006.06.01216859932

20. 

M DaveM AtturG PalmerThe antioxidant resveratrol protects against chondrocyte apoptosis via effects on mitochondrial polarization and ATP productionArthritis Rheum5827862797200810.1002/art.2379918759268

21. 

A LiaciniJ SylvesterWQ LiInduction of matrix metalloproteinase-13 gene expression by TNF-alpha is mediated by MAP kinases, AP-1, and NF-kappaB transcription factors in articular chondrocytesExp Cell Res288208217200310.1016/S0014-4827(03)00180-012878172

22. 

DD WoodEJ IhrieCA DinarelloPL CohenIsolation of an interleukin-1-like factor from human joint effusionsArthritis Rheum26975983198310.1002/art.17802608066603852

23. 

R LargoMA Alvarez-SoriaI Diez-OrtegoGlucosamine inhibits IL-1beta-induced NFkappaB activation in human osteoarthritic chondrocytesOsteoarthritis Cartilage11290298200310.1016/S1063-4584(03)00028-112681956

24. 

AR AminM DaveM AtturSB AbramsonCOX-2, NO, and cartilage damage and repairCurr Rheumatol Rep2447453200010.1007/s11926-000-0019-511123096

25. 

JY ReginsterR DeroisyLC RovatiLong-term effects of glucosamine sulphate on osteoarthritis progression: a randomised, placebo-controlled clinical trialLancet357251256200110.1016/S0140-6736(00)03610-211214126

26. 

R SinghS AhmedN IslamVM GoldbergTM HaqqiEpigallocatechin-3-gallate inhibits interleukin-1beta–induced expression of nitric oxide synthase and production of nitric oxide in human chondrocytes: suppression of nuclear factor kappaB activation by degradation of the inhibitor of nuclear factor kappaBArthritis Rheum46207920862002

27. 

PS BurrageKS MixCE BrinckerhoffMatrix metalloproteinases: role in arthritisFront Biosci11529543200610.2741/181716146751

28. 

J Martel-PelletierDJ WelschJP PelletierMetalloproteases and inhibitors in arthritic diseasesBest Pract Res Clin Rheumatol15805829200110.1053/berh.2001.019511812023

29. 

M ShakibaeiT JohnG Schulze-TanzilI LehmannA MobasheriSuppression of NF-kappaB activation by curcumin leads to inhibition of expression of cyclo-oxygenase-2 and matrix metalloproteinase-9 in human articular chondrocytes: implications for the treatment of osteoarthritisBiochem Pharmacol7314341445200710.1016/j.bcp.2007.01.005

30. 

PJ BarnesM KarinNuclear factor-κB: A pivotal transcription factor in chronic inflammatory diseasesN Engl J Med336106610711997

31. 

L SuomalainenV PentikäinenL DunkelSphingosine-1-phosphate inhibits nuclear factor κB activation and germ cell apoptosis in the human testis independently of its receptorsAm J Pathol1667737812005

32. 

K MasukoM MurataH NakamuraK YudohK NishiokaT KatoSphingosine-1-phosphate attenuates proteoglycan aggrecan expression via production of prostaglandin E2 from human articular chondrocytesBMC Musculoskelet Disord829200710.1186/1471-2474-8-2917374154

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Moon M, Jeong J, Lee , Seol J and Park S: Sphingosine-1-phosphate inhibits interleukin-1β-induced inflammation in human articular chondrocytes. Int J Mol Med 30: 1451-1458, 2012.
APA
Moon, M., Jeong, J., Lee, ., Seol, J., & Park, S. (2012). Sphingosine-1-phosphate inhibits interleukin-1β-induced inflammation in human articular chondrocytes. International Journal of Molecular Medicine, 30, 1451-1458. https://doi.org/10.3892/ijmm.2012.1135
MLA
Moon, M., Jeong, J., Lee, ., Seol, J., Park, S."Sphingosine-1-phosphate inhibits interleukin-1β-induced inflammation in human articular chondrocytes". International Journal of Molecular Medicine 30.6 (2012): 1451-1458.
Chicago
Moon, M., Jeong, J., Lee, ., Seol, J., Park, S."Sphingosine-1-phosphate inhibits interleukin-1β-induced inflammation in human articular chondrocytes". International Journal of Molecular Medicine 30, no. 6 (2012): 1451-1458. https://doi.org/10.3892/ijmm.2012.1135
Copy and paste a formatted citation
x
Spandidos Publications style
Moon M, Jeong J, Lee , Seol J and Park S: Sphingosine-1-phosphate inhibits interleukin-1β-induced inflammation in human articular chondrocytes. Int J Mol Med 30: 1451-1458, 2012.
APA
Moon, M., Jeong, J., Lee, ., Seol, J., & Park, S. (2012). Sphingosine-1-phosphate inhibits interleukin-1β-induced inflammation in human articular chondrocytes. International Journal of Molecular Medicine, 30, 1451-1458. https://doi.org/10.3892/ijmm.2012.1135
MLA
Moon, M., Jeong, J., Lee, ., Seol, J., Park, S."Sphingosine-1-phosphate inhibits interleukin-1β-induced inflammation in human articular chondrocytes". International Journal of Molecular Medicine 30.6 (2012): 1451-1458.
Chicago
Moon, M., Jeong, J., Lee, ., Seol, J., Park, S."Sphingosine-1-phosphate inhibits interleukin-1β-induced inflammation in human articular chondrocytes". International Journal of Molecular Medicine 30, no. 6 (2012): 1451-1458. https://doi.org/10.3892/ijmm.2012.1135
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team