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December 2012 Volume 30 Issue 6

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Article

Proteomic analysis of changes in protein expression in serum from animals exposed to paraquat

  • Authors:
    • Yong-Sik Kim
    • Hana Jung
    • Hyo-Wook Gil
    • Sae-Yong Hong
    • Ho-Yeon Song
  • View Affiliations / Copyright

    Affiliations: Department of Microbiology, College of Medicine, Soon Chun Hyang University, Cheonan, Chungnam 330-090, Republic of Korea, Department of Internal Medicine, Soon Chun Hyang Cheonan Hospital, Cheonan, Republic of Korea
  • Pages: 1521-1527
    |
    Published online on: September 27, 2012
       https://doi.org/10.3892/ijmm.2012.1143
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Abstract

Paraquat (PQ) poisoning remains a major public health concern in many countries. Extensive research has focused on finding early diagnostic biomarkers of acute PQ poisoning. In order to investigate the characterization of diagnostic biomarkers in PQ poisoning, we utilized proteomic analysis using serum from rats exposed to PQ, and we identified 8 differentially expressed proteins from over 500 protein spots. The expression of apolipoprotein E (ApoE), preprohaptoglobin (Pphg), a precursor of haptoglobin (Hp), and complement component 3 (C3) proteins was greatly induced by PQ exposure while the expression of fibrinogen γ-chain (FGG) and Ac-158 was dramatically reduced. To further investigate the possibility of ApoE, Pphg and FGG as useful diagnostic biomarkers of PQ poisoning, western blot and qRT-PCR analyses were conducted using cell lines as well as rat and human sera. The expression levels of ApoE, Hp and FGG were significantly altered in the presence of PQ in both rat and human serum suggesting that these proteins may be appropriate candidate molecular biomarkers for the early diagnosis of acute PQ intoxication.
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1. 

IB GawarammanaNA BuckleyMedical management of paraquat ingestionBr J Clin Pharmacol72745757201110.1111/j.1365-2125.2011.04026.x21615775

2. 

I AhmadA KumarS ShuklaH Prasad PandeyC SinghThe involvement of nitric oxide in maneb- and paraquat-induced oxidative stress in rat polymorphonuclear leukocytesFree Radic Res42849862200810.1080/1071576080251373318985485

3. 

ZE SuntresRole of antioxidants in paraquat toxicityToxicology1806577200210.1016/S0300-483X(02)00382-712324200

4. 

P HouzeFJ BaudR MouyC BismuthR BourdonJM ScherrmannToxicokinetics of paraquat in humansHum Exp Toxicol9512199010.1177/096032719000900103

5. 

SM PondLP RivoryEC HampsonMS RobertsKinetics of toxic doses of paraquat and the effects of hemoperfusion in the dogJ Toxicol Clin Toxicol31229246199310.3109/155636593090003918492337

6. 

P IndovinaE MarcelliP MarantaG TarroLung cancer proteomics: recent advances in biomarker discoveryInt J Proteomics2011726869201110.1155/2011/72686922229091

7. 

AT LauJF ChiuBiomarkers of lung-related diseases: current knowledge by proteomic approachesJ Cell Physiol221535543200910.1002/jcp.2189319681054

8. 

A PlymothP HainautProteomics beyond proteomics: toward clinical applicationsCurr Opin Oncol237782201110.1097/CCO.0b013e32834179c121107258

9. 

P GovenderMJ DunnSC DonnellyProteomics and the lung: Analysis of bronchoalveolar lavage fluidProteomics Clin Appl310441051200910.1002/prca.20090003221137005

10. 

T OkamotoY MiyazakiR ShirahamaM TamaokaN InaseProteome analysis of bronchoalveolar lavage fluid in chronic hypersensitivity pneumonitisAllergol Int618392201210.2332/allergolint.11-OA-031522015564

11. 

HJ IssaqZ XiaoTD VeenstraSerum and plasma proteomicsChem Rev10736013620200710.1021/cr068287r17636887

12. 

BR OakleyDR KirschNR MorrisA simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gelsAnal Biochem105361363198010.1016/0003-2697(80)90470-46161559

13. 

A ShevchenkoM WilmO VormM MannMass spectrometric sequencing of proteins silver-stained polyacrylamide gelsAnal Chem68850858199610.1021/ac950914h8779443

14. 

JH KimHW GilJO YangEY LeeSY HongSerum uric acid level as a marker for mortality and acute kidney injury in patients with acute paraquat intoxicationNephrol Dial Transplant2618461852201110.1093/ndt/gfq63220966188

15. 

CD YeoJW KimYO KimSA YoonKH KimYS KimThe role of pentraxin-3 as a prognostic biomarker in paraquat poisoningToxicol Lett212157160201122210019

16. 

F YangAJ GhioDC HerbertFJ WeakerCA WalterJJ CoalsonPulmonary expression of the human haptoglobin geneAm J Respir Cell Mol Biol23277282200010.1165/ajrcmb.23.3.4069

17. 

E SalomonssonVL ThijssenAW GriffioenUJ NilssonH LefflerThe anti-angiogenic peptide anginex greatly enhances galectin-1 binding affinity for glycoproteinsJ Biol Chem2861380113804201110.1074/jbc.C111.22909621372130

18. 

HY TsaiK BoonyapranaiS SriyamGlycoproteomics analysis to identify a glycoform on haptoglobin associated with lung cancerProteomics1121622170201110.1002/pmic.20100031921538882

19. 

P LewisN StefanovicJ PeteLack of the antioxidant enzyme glutathione peroxidase-1 accelerates atherosclerosis in diabetic apolipoprotein E-deficient miceCirculation11521782187200710.1161/CIRCULATIONAHA.106.66425017420349

20. 

SQ LiJ YunFB XueComparative proteome analysis of serum from acute pulmonary embolism rat model for biomarker discoveryJ Proteome Res6150159200710.1021/pr060310217203959

21. 

D KarthikS IlavenilB KaleeswaranS SunilS RavikumarProteomic analysis of plasma proteins in diabetic rats by 2D electrophoresis and MALDI-TOF-MSAppl Biochem Biotechnol16615071519201210.1007/s12010-012-9544-822258647

22. 

Y AhmadD ShuklaI GargIdentification of haptoglobin and apolipoprotein A–I as biomarkers for high altitude pulmonary edemaFunct Integr Genomics114074172011

23. 

HH NgaiWH SitPP JiangV ThongboonkerdJM WanMarkedly increased urinary preprohaptoglobin and haptoglobin in passive Heymann nephritis: a differential proteomics approachJ Proteome Res633133320200710.1021/pr070245b17616219

24. 

K ImaizumiDiet and atherosclerosis in apolipoprotein E-deficient miceBiosci Biotechnol Biochem7510231035201110.1271/bbb.11005921670535

25. 

L CiglianoCR PuglieseMS SpagnuoloR PalumboP AbresciaHaptoglobin binds the antiatherogenic protein apolipoprotein E - impairment of apolipoprotein E stimulation of both lecithin: cholesterol acyltransferase activity and cholesterol uptake by hepatocytesFEBS J27661586171200910.1111/j.1742-4658.2009.07319.x

26. 

C CuthbertZ WangX ZhangSP TamRegulation of human apolipoprotein A–I gene expression by gramoxoneJ Biol Chem27214954149601997

27. 

L JingCE ParkerD SeoDiscovery of biomarker candidates for coronary artery disease from an APOE-knock out mouse model using iTRAQ-based multiplex quantitative proteomicsProteomics1127632776201110.1002/pmic.20100020221681990

28. 

M Santos-GonzalezC Gomez DiazP NavasJM VillalbaModifications of plasma proteome in long-lived rats fed on a coenzyme Q10-supplemented dietExp Gerontol42798806200710.1016/j.exger.2007.04.01317587521

29. 

B RiahiH RafatpanahM MahmoudiEvaluation of suppressive effects of paraquat on innate immunity in Balb/c miceJ Immunotoxicol83945201110.3109/1547691X.2010.54309521299353

30. 

S SunH WangG ZhaoComplement inhibition alleviates paraquat-induced acute lung injuryAm J Respir Cell Mol Biol45834842201110.1165/rcmb.2010-0444OC21421909

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Copy and paste a formatted citation
Spandidos Publications style
Kim Y, Jung H, Gil H, Hong S and Song H: Proteomic analysis of changes in protein expression in serum from animals exposed to paraquat. Int J Mol Med 30: 1521-1527, 2012.
APA
Kim, Y., Jung, H., Gil, H., Hong, S., & Song, H. (2012). Proteomic analysis of changes in protein expression in serum from animals exposed to paraquat. International Journal of Molecular Medicine, 30, 1521-1527. https://doi.org/10.3892/ijmm.2012.1143
MLA
Kim, Y., Jung, H., Gil, H., Hong, S., Song, H."Proteomic analysis of changes in protein expression in serum from animals exposed to paraquat". International Journal of Molecular Medicine 30.6 (2012): 1521-1527.
Chicago
Kim, Y., Jung, H., Gil, H., Hong, S., Song, H."Proteomic analysis of changes in protein expression in serum from animals exposed to paraquat". International Journal of Molecular Medicine 30, no. 6 (2012): 1521-1527. https://doi.org/10.3892/ijmm.2012.1143
Copy and paste a formatted citation
x
Spandidos Publications style
Kim Y, Jung H, Gil H, Hong S and Song H: Proteomic analysis of changes in protein expression in serum from animals exposed to paraquat. Int J Mol Med 30: 1521-1527, 2012.
APA
Kim, Y., Jung, H., Gil, H., Hong, S., & Song, H. (2012). Proteomic analysis of changes in protein expression in serum from animals exposed to paraquat. International Journal of Molecular Medicine, 30, 1521-1527. https://doi.org/10.3892/ijmm.2012.1143
MLA
Kim, Y., Jung, H., Gil, H., Hong, S., Song, H."Proteomic analysis of changes in protein expression in serum from animals exposed to paraquat". International Journal of Molecular Medicine 30.6 (2012): 1521-1527.
Chicago
Kim, Y., Jung, H., Gil, H., Hong, S., Song, H."Proteomic analysis of changes in protein expression in serum from animals exposed to paraquat". International Journal of Molecular Medicine 30, no. 6 (2012): 1521-1527. https://doi.org/10.3892/ijmm.2012.1143
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