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December 2012 Volume 30 Issue 6

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Article

Sodium butyrate restores ASC expression and induces apoptosis in LS174T cells

  • Authors:
    • Shuai Zhang
    • Jingqiao Bai
    • Shuangyi Ren
    • Ran Wang
    • Li Zhang
    • Yunfei Zuo
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Biochemistry, Dalian Medical University, Dalian 116044, P.R. China, Department of Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian 116023, P.R. China
  • Pages: 1431-1437
    |
    Published online on: October 15, 2012
       https://doi.org/10.3892/ijmm.2012.1156
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Abstract

Sodium butyrate (NaBu) is a short-chain fatty acid (SCFA), which has been proposed as a potential anticancer agent. Apoptosis-associated speck-like protein (ASC) is a pro-apoptotic signaling factor that is subjected to epigenetic silencing in human cancers. Modulation by the aberrant methylation of CpG islands of ASC is a well-characterized epigenetic mechanism, and the methylation-induced silencing of ASC has been observed in several types of tumors. NaBu induces cell cycle arrest, markers of cell differentiation and apoptosis in colon cancer. NaBu promotes transcriptional activation by relaxing the DNA conformation and displays anti-proliferative and differentiating activity in a wide variety of cancers. Thus, we used NaBu to investigate the relationship between the status of cell proliferation and the re-expression of ASC in colon carcinoma LS174T cells. Our experiments determined ASC re-expression at the protein level using western blotting. In addition, we used reverse transcription-polymerase chain reaction to detect the expression levels of ASC mRNA and an MTT assay to detect the inhibitory rate of cell growth. The apoptosis rate was also detected for further validation of the re-expression of ASC. The results showed that ASC re-expression was significantly increased in the LS174 cells following NaBu treatment in a time- and dose-dependent manner. The expression of ASC also induced the apoptosis of LS174T cells. These results suggest that NaBu plays a role in the reactivation of ASC expression and that the latter promotes the apoptosis of LS174T cells.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang S, Bai J, Ren S, Wang R, Zhang L and Zuo Y: Sodium butyrate restores ASC expression and induces apoptosis in LS174T cells. Int J Mol Med 30: 1431-1437, 2012.
APA
Zhang, S., Bai, J., Ren, S., Wang, R., Zhang, L., & Zuo, Y. (2012). Sodium butyrate restores ASC expression and induces apoptosis in LS174T cells. International Journal of Molecular Medicine, 30, 1431-1437. https://doi.org/10.3892/ijmm.2012.1156
MLA
Zhang, S., Bai, J., Ren, S., Wang, R., Zhang, L., Zuo, Y."Sodium butyrate restores ASC expression and induces apoptosis in LS174T cells". International Journal of Molecular Medicine 30.6 (2012): 1431-1437.
Chicago
Zhang, S., Bai, J., Ren, S., Wang, R., Zhang, L., Zuo, Y."Sodium butyrate restores ASC expression and induces apoptosis in LS174T cells". International Journal of Molecular Medicine 30, no. 6 (2012): 1431-1437. https://doi.org/10.3892/ijmm.2012.1156
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang S, Bai J, Ren S, Wang R, Zhang L and Zuo Y: Sodium butyrate restores ASC expression and induces apoptosis in LS174T cells. Int J Mol Med 30: 1431-1437, 2012.
APA
Zhang, S., Bai, J., Ren, S., Wang, R., Zhang, L., & Zuo, Y. (2012). Sodium butyrate restores ASC expression and induces apoptosis in LS174T cells. International Journal of Molecular Medicine, 30, 1431-1437. https://doi.org/10.3892/ijmm.2012.1156
MLA
Zhang, S., Bai, J., Ren, S., Wang, R., Zhang, L., Zuo, Y."Sodium butyrate restores ASC expression and induces apoptosis in LS174T cells". International Journal of Molecular Medicine 30.6 (2012): 1431-1437.
Chicago
Zhang, S., Bai, J., Ren, S., Wang, R., Zhang, L., Zuo, Y."Sodium butyrate restores ASC expression and induces apoptosis in LS174T cells". International Journal of Molecular Medicine 30, no. 6 (2012): 1431-1437. https://doi.org/10.3892/ijmm.2012.1156
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