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Article

Centella asiatica protects against UVB-induced HaCaT keratinocyte damage through microRNA expression changes

  • Authors:
    • In-Sook An
    • Sungkwan An
    • Tae-Βoo Choe
    • Sang-Μo Kang
    • Jae Ho Lee
    • In-Chul Park
    • Young-Woo Jin
    • Su-Jae Lee
    • Seunghee Bae
  • View Affiliations / Copyright

    Affiliations: Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143-701, Republic of Korea, Department of Microbial Engineering, Konkuk University, Seoul 143-701, Republic of Korea, Laboratory of Molecular Oncology, Cheil General Hospital and Women's Healthcare Center, Kwandong University, College of Medicine, Seoul 100-380, Republic of Korea, Laboratory of Functional Genomics, Korea Institute of Radiological and Medical Sciences, Seoul 139-706, Republic of Korea, Division of Radiation Effect Research, Radiation Health Research Institute of KHNP, Seoul 132-703, Republic of Korea, Department of Chemistry, Hanyang University, Seoul 133-791, Republic of Korea, Molecular-Targeted Drug Research Center, Konkuk University, Seoul 143-701, Republic of Korea
  • Pages: 1349-1356
    |
    Published online on: October 15, 2012
       https://doi.org/10.3892/ijmm.2012.1157
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Abstract

This study aimed to evaluate the protective effects of Centella asiatica (C. asiatica) against ultraviolet B (UVB) damage in human keratinocytes using microRNA (miRNA) expression profiling analysis. Titrated extract of C. asiatica (TECA) demonstrated low cytotoxicity in normal human HaCaT keratinocytes only at low doses (<5 µg/ml). UVB (50 mJ/cm2) irradiation significantly decreased cell viability, and TECA treatment decreased the UVB toxicity. By using miRNA microarrays, we determined that 72 miRNAs had an altered expression following TECA treatment in UVB-irradiated keratinocytes (46 upregulated and 26 downregulated). Using an miRNA target gene prediction tool and Gene Ontology (GO) analysis, we determined that miRNAs with altered expression were functionally related with the inhibition of apoptosis and cell proliferation. Overall, these results provide meaningful information to facilitate the understanding of TECA-mediated UVB protection in human keratinocytes.
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1. 

V MuthusamyTJ PivaThe UV response of the skin: a review of the MAPK, NFkappaB and TNFalpha signal transduction pathwaysArch Dermatol Res302517201010.1007/s00403-009-0994-y19756672

2. 

GJ ClydesdaleGW DandieHK MullerUltraviolet light induced injury: immunological and inflammatory effectsImmunol Cell Biol79547568200110.1046/j.1440-1711.2001.01047.x11903614

3. 

K BenderM GottlicherS WhitesideHJ RahmsdorfP HerrlichSequential DNA damage-independent and -dependent activation of NF-kappaB by UVEMBO J1751705181199810.1093/emboj/17.17.51709724653

4. 

DA LewisDF SpandauUVB activation of NF-kappaB in normal human keratinocytes occurs via a unique mechanismArch Dermatol Res29993101200710.1007/s00403-006-0729-217256146

5. 

N LiM KarinIonizing radiation and short wavelength UV activate NF-kappaB through two distinct mechanismsProc Natl Acad Sci USA951301213017199810.1073/pnas.95.22.130129789032

6. 

ZQ YuanRI FeldmanM SunInhibition of JNK by cellular stress- and tumor necrosis factor alpha-induced AKT2 through activation of the NF kappa B pathway in human epithelial CellsJ Biol Chem2772997329982200210.1074/jbc.M20363620012048203

7. 

J LiuD YangY MinemotoM LeitgesMR RosnerA LinNF-kappaB is required for UV-induced JNK activation via induction of PKCdeltaMol Cell21467480200610.1016/j.molcel.2005.12.02016483929

8. 

H KimJS KangWJ LeeThe production IL-21 and VEGF in UVB-irradiated human keratinocyte cell line, HaCaTImmune Netw107580201010.4110/in.2010.10.2.7520532127

9. 

DS ChangSJ SeoCK HongThe effect of amniotic membrane extract on the expression of iNOS mRNA and generation of NO in HaCaT cell by ultraviolet B irradiationPhotodermatol Photoimmunol Photomed18280286200210.1034/j.1600-0781.2002.02752.x12535023

10. 

V AmbrosRC LeeIdentification of microRNAs and other tiny noncoding RNAs by cDNA cloningMethods Mol Biol265131158200415103073

11. 

AM ChengMW ByromJ SheltonLP FordAntisense inhibition of human miRNAs and indications for an involvement of miRNA in cell growth and apoptosisNucleic Acids Res3312901297200510.1093/nar/gki20015741182

12. 

JF ChenEM MandelJM ThomsonThe role of microRNA-1 and microRNA-133 in skeletal muscle proliferation and differentiationNat Genet38228233200610.1038/ng172516380711

13. 

WJ ChoJM ShinJS KimmiR-372 regulates cell cycle and apoptosis of ags human gastric cancer cell line through direct regulation of LATS2Mol Cells28521527200910.1007/s10059-009-0158-019937137

14. 

LM HolstB KaczkowskiR GniadeckiReproducible pattern of microRNA in normal human skinExp Dermatol19e201e205201010.1111/j.1600-0625.2009.01049.x20201961

15. 

LM HolstB KaczkowskiM GludE Futoma-KazmierczakLF HansenR GniadeckiThe microRNA molecular signature of atypic and common acquired melanocytic nevi: differential expression of miR-125b and let-7cExp Dermatol20278280201110.1111/j.1600-0625.2010.01163.x21166724

16. 

A IchiharaM JinninK YamanemicroRNA-mediated keratinocyte hyperproliferation in Psoriasis vulgarisBr J Dermatol16510031010201121711342

17. 

N XuP BrodinT WeiMiR-125b, a microRNA down-regulated in psoriasis, modulates keratinocyte proliferation by targeting FGFR2J Invest Dermatol13115211529201110.1038/jid.2011.5521412257

18. 

J HildebrandM RutzeN WalzA comprehensive analysis of microRNA expression during human keratinocyte differentiation in vitro and in vivoJ Invest Dermatol1312029201110.1038/jid.2010.26820827281

19. 

BR ZhouY XuF PermatasariCharacterization of the miRNA profile in UVB-irradiated normal human keratinocytesExp Dermatol21317319201210.1111/j.1600-0625.2012.01465.x22417313

20. 

B BrinkhausM LindnerD SchuppanEG HahnChemical, pharmacological and clinical profile of the East Asian medical plant Centella asiaticaPhytomedicine7427448200010.1016/S0944-7113(00)80065-311081995

21. 

FX MaquartF ChastangA SimeonP BirembautP GilleryY WegrowskiTriterpenes from Centella asiatica stimulate extracellular matrix accumulation in rat experimental woundsEur J Dermatol92892961999

22. 

G JayashreeG Kurup MuraleedharaS SudarslalVB JacobAnti-oxidant activity of Centella asiatica on lymphoma-bearing miceFitoterapia744314342003

23. 

TD BabuG KuttanJ PadikkalaCytotoxic and anti-tumour properties of certain taxa of Umbelliferae with special reference to Centella asiatica (L.) UrbanJ Ethnopharmacol485357199510.1016/0378-8741(95)01284-K8569247

24. 

YJ KimHJ ChaKH NamY YoonH LeeS AnCentella asiatica extracts modulate hydrogen peroxide-induced senescence in human dermal fibroblastsExp Dermatol209981003201110.1111/j.1600-0625.2011.01388.x

25. 

P HashimH SidekMH HelanA SaberyUD PalanisamyM IlhamTriterpene composition and bioactivities of Centella asiaticaMolecules1613101322201110.3390/molecules1602131021278681

26. 

RS PillaiSN BhattacharyyaW FilipowiczRepression of protein synthesis by miRNAs: how many mechanisms?Trends Cell Biol17118126200710.1016/j.tcb.2006.12.00717197185

27. 

L LuK YingS WeiAsiaticoside induction for cell-cycle progression, proliferation and collagen synthesis in human dermal fibroblastsInt J Dermatol43801807200410.1111/j.1365-4632.2004.02047.x15533060

28. 

JS GuoCL ChengMW KooInhibitory effects of Centella asiatica water extract and asiaticoside on inducible nitric oxide synthase during gastric ulcer healing in ratsPlanta Med70115011542004

29. 

BC ParkKO BosireES LeeYS LeeJA KimAsiatic acid induces apoptosis in SK-MEL-2 human melanoma cellsCancer Lett2188190200510.1016/j.canlet.2004.06.03915639343

30. 

S BabykuttyJ PadikkalaPP SathiadevanApoptosis induction of Centella asiatica on human breast cancer cellsAfr J Tradit Complement Altern Med69162008

31. 

LT LinLT LiuLC ChiangCC LinIn vitro anti-hepatoma activity of fifteen natural medicines from CanadaPhytother Res16440444200210.1002/ptr.93712203264

32. 

M YoshidaM FuchigamiT NagaoAntiproliferative constituents from Umbelliferae plants VIIActive triterpenes and rosmarinic acid from Centella asiatica Biol Pharm Bull28173175200515635187

33. 

JH SampsonA RamanG KarlsenH NavsariaIM LeighIn vitro keratinocyte antiproliferant effect of Centella asiatica extract and triterpenoid saponinsPhytomedicine8230235200110.1078/0944-7113-0003211417919

34. 

BH RuszymahSR ChowdhuryNA MananOS FongMI AdenanAB SaimAqueous extract of Centella asiatica promotes corneal epithelium wound healing in vitroJ Ethnopharmacol1403333382012

35. 

P Rivetti di Val CervoAM LenaM Nicolosop63-microRNA feedback in keratinocyte senescenceProc Natl Acad Sci USA10911331138201222228303

36. 

Y XuB ZhouDi WuZ YinD LuoBaicalin modulates microRNA expression in UVB irradiated mouse skinJ Biomed Res26125134201210.1016/S1674-8301(12)60022-023554741

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Copy and paste a formatted citation
Spandidos Publications style
An I, An S, Choe T, Kang S, Lee JH, Park I, Jin Y, Lee S and Bae S: Centella asiatica protects against UVB-induced HaCaT keratinocyte damage through microRNA expression changes. Int J Mol Med 30: 1349-1356, 2012.
APA
An, I., An, S., Choe, T., Kang, S., Lee, J.H., Park, I. ... Bae, S. (2012). Centella asiatica protects against UVB-induced HaCaT keratinocyte damage through microRNA expression changes. International Journal of Molecular Medicine, 30, 1349-1356. https://doi.org/10.3892/ijmm.2012.1157
MLA
An, I., An, S., Choe, T., Kang, S., Lee, J. H., Park, I., Jin, Y., Lee, S., Bae, S."Centella asiatica protects against UVB-induced HaCaT keratinocyte damage through microRNA expression changes". International Journal of Molecular Medicine 30.6 (2012): 1349-1356.
Chicago
An, I., An, S., Choe, T., Kang, S., Lee, J. H., Park, I., Jin, Y., Lee, S., Bae, S."Centella asiatica protects against UVB-induced HaCaT keratinocyte damage through microRNA expression changes". International Journal of Molecular Medicine 30, no. 6 (2012): 1349-1356. https://doi.org/10.3892/ijmm.2012.1157
Copy and paste a formatted citation
x
Spandidos Publications style
An I, An S, Choe T, Kang S, Lee JH, Park I, Jin Y, Lee S and Bae S: Centella asiatica protects against UVB-induced HaCaT keratinocyte damage through microRNA expression changes. Int J Mol Med 30: 1349-1356, 2012.
APA
An, I., An, S., Choe, T., Kang, S., Lee, J.H., Park, I. ... Bae, S. (2012). Centella asiatica protects against UVB-induced HaCaT keratinocyte damage through microRNA expression changes. International Journal of Molecular Medicine, 30, 1349-1356. https://doi.org/10.3892/ijmm.2012.1157
MLA
An, I., An, S., Choe, T., Kang, S., Lee, J. H., Park, I., Jin, Y., Lee, S., Bae, S."Centella asiatica protects against UVB-induced HaCaT keratinocyte damage through microRNA expression changes". International Journal of Molecular Medicine 30.6 (2012): 1349-1356.
Chicago
An, I., An, S., Choe, T., Kang, S., Lee, J. H., Park, I., Jin, Y., Lee, S., Bae, S."Centella asiatica protects against UVB-induced HaCaT keratinocyte damage through microRNA expression changes". International Journal of Molecular Medicine 30, no. 6 (2012): 1349-1356. https://doi.org/10.3892/ijmm.2012.1157
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