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Article

Inhibitory effect of butein on tumor necrosis factor-α-induced expression of cell adhesion molecules in human lung epithelial cells via inhibition of reactive oxygen species generation, NF-κB activation and Akt phosphorylation

  • Authors:
    • Ji Hoon Jang
    • Eun Sun Yang
    • Kyoung-Jin Min
    • Taeg Kyu Kwon
  • View Affiliations / Copyright

    Affiliations: Department of Immunology, School of Medicine, Keimyung University, Daegu, Republic of Korea
  • Pages: 1357-1364
    |
    Published online on: October 15, 2012
       https://doi.org/10.3892/ijmm.2012.1158
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Abstract

Cell adhesion molecules play an important role in inflammatory response, angiogenesis and tumor progression. Butein (tetrahydroxychalcone) is a small molecule from natural sources, known to be a potential therapeutic drug with anti-inflammatory, anticancer and antioxidant activities. In the present study, we investigated the inhibitory effect of butein on tumor necrosis factor (TNF)-α-induced adhesion molecule expression and its molecular mechanism of action. Butein significantly decreased TNF-α-induced monocyte (U937) cell adhesion to lung epithelial cells in a dose-dependent manner. Butein also inhibited the protein and mRNA expression of intercellular cell adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in TNF-α-stimulated A549 human lung epithelial cells in a dose-dependent manner. Butein inhibited TNF-α-induced reactive oxygen species (ROS) generation and nuclear factor-κB (NF-κB) activation in A549 cells; it also inhibited the phosphorylation of MAPKs and Akt, suggesting that the MAPK/Akt signaling pathway may be involved in the butein-mediated inhibition of TNF-α-induced leukocyte adhesion to A549 cells. Collectively, our results suggest that butein affects cell adhesion through the inhibition of TNF-α-induced ICAM-1 and VCAM-1 expression by inhibiting the NF-κB/MAPK/Akt signaling pathway and ROS generation, thereby, elucidating the role of butein in the anti-inflammatory response.
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Copy and paste a formatted citation
Spandidos Publications style
Jang JH, Yang ES, Min K and Kwon TK: Inhibitory effect of butein on tumor necrosis factor-α-induced expression of cell adhesion molecules in human lung epithelial cells via inhibition of reactive oxygen species generation, NF-κB activation and Akt phosphorylation. Int J Mol Med 30: 1357-1364, 2012.
APA
Jang, J.H., Yang, E.S., Min, K., & Kwon, T.K. (2012). Inhibitory effect of butein on tumor necrosis factor-α-induced expression of cell adhesion molecules in human lung epithelial cells via inhibition of reactive oxygen species generation, NF-κB activation and Akt phosphorylation. International Journal of Molecular Medicine, 30, 1357-1364. https://doi.org/10.3892/ijmm.2012.1158
MLA
Jang, J. H., Yang, E. S., Min, K., Kwon, T. K."Inhibitory effect of butein on tumor necrosis factor-α-induced expression of cell adhesion molecules in human lung epithelial cells via inhibition of reactive oxygen species generation, NF-κB activation and Akt phosphorylation". International Journal of Molecular Medicine 30.6 (2012): 1357-1364.
Chicago
Jang, J. H., Yang, E. S., Min, K., Kwon, T. K."Inhibitory effect of butein on tumor necrosis factor-α-induced expression of cell adhesion molecules in human lung epithelial cells via inhibition of reactive oxygen species generation, NF-κB activation and Akt phosphorylation". International Journal of Molecular Medicine 30, no. 6 (2012): 1357-1364. https://doi.org/10.3892/ijmm.2012.1158
Copy and paste a formatted citation
x
Spandidos Publications style
Jang JH, Yang ES, Min K and Kwon TK: Inhibitory effect of butein on tumor necrosis factor-α-induced expression of cell adhesion molecules in human lung epithelial cells via inhibition of reactive oxygen species generation, NF-κB activation and Akt phosphorylation. Int J Mol Med 30: 1357-1364, 2012.
APA
Jang, J.H., Yang, E.S., Min, K., & Kwon, T.K. (2012). Inhibitory effect of butein on tumor necrosis factor-α-induced expression of cell adhesion molecules in human lung epithelial cells via inhibition of reactive oxygen species generation, NF-κB activation and Akt phosphorylation. International Journal of Molecular Medicine, 30, 1357-1364. https://doi.org/10.3892/ijmm.2012.1158
MLA
Jang, J. H., Yang, E. S., Min, K., Kwon, T. K."Inhibitory effect of butein on tumor necrosis factor-α-induced expression of cell adhesion molecules in human lung epithelial cells via inhibition of reactive oxygen species generation, NF-κB activation and Akt phosphorylation". International Journal of Molecular Medicine 30.6 (2012): 1357-1364.
Chicago
Jang, J. H., Yang, E. S., Min, K., Kwon, T. K."Inhibitory effect of butein on tumor necrosis factor-α-induced expression of cell adhesion molecules in human lung epithelial cells via inhibition of reactive oxygen species generation, NF-κB activation and Akt phosphorylation". International Journal of Molecular Medicine 30, no. 6 (2012): 1357-1364. https://doi.org/10.3892/ijmm.2012.1158
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