microRNA changes in the dorsal horn of the spinal cord of rats with chronic constriction injury: A TaqMan® Low Density Array study

  • Authors:
    • Yuuki Genda
    • Masae Arai
    • Masashi Ishikawa
    • Shunsuke Tanaka
    • Tadashi Okabe
    • Atsuhiro Sakamoto
  • View Affiliations

  • Published online on: October 24, 2012     https://doi.org/10.3892/ijmm.2012.1163
  • Pages: 129-137
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Abstract

Elucidation of the mechanisms underlying neuropathic pain is expected to aid in the discovery and selection of effective therapeutic methods. Currently, microRNA (miRNA) is thought to play an important role in the development and maintenance of the nervous system. We, therefore, hypothesized that miRNAs are involved in neuropathic pain, and investigated this possibility by analyzing miRNA expression in the dorsal horn of the spinal cord in a chronic constriction injury (CCI) rat model using the TaqMan® Low Density Array (TLDA). Neuropathic pain model rats were produced by CCI induced by ligation of the sciatic nerve. The miRNA expression in the dorsal horn of the spinal cord was analyzed in Day 0 rats, with no sciatic nerve ligation or sham operation, Day 7 rats, examined 7 days after sciatic nerve ligation or sham operation, and Day 14 rats, examined 14 days after sciatic nerve ligation or sham operation using TLDA. In this study, 111 miRNAs were significantly regulated in CCI rats in both the Day 7 and Day 14 groups compared with sham rats in both groups. Of these 111, there were 75 miRNAs (67.6%) that had been analyzed in previous reports and 36 miRNAs (32.4%) related to the development of tumors of the nervous system and neurodegenerative diseases. Certain miRNAs were reported to be related to neuropathic pain; miR-500, -221 and -21. The expression levels of a large number of miRNAs in the dorsal horn of the spinal cord in CCI rats changed. These results provide a step toward elucidation of the mechanisms underlying neuropathic pain.
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January 2013
Volume 31 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Genda Y, Arai M, Ishikawa M, Tanaka S, Okabe T and Sakamoto A: microRNA changes in the dorsal horn of the spinal cord of rats with chronic constriction injury: A TaqMan® Low Density Array study. Int J Mol Med 31: 129-137, 2013
APA
Genda, Y., Arai, M., Ishikawa, M., Tanaka, S., Okabe, T., & Sakamoto, A. (2013). microRNA changes in the dorsal horn of the spinal cord of rats with chronic constriction injury: A TaqMan® Low Density Array study. International Journal of Molecular Medicine, 31, 129-137. https://doi.org/10.3892/ijmm.2012.1163
MLA
Genda, Y., Arai, M., Ishikawa, M., Tanaka, S., Okabe, T., Sakamoto, A."microRNA changes in the dorsal horn of the spinal cord of rats with chronic constriction injury: A TaqMan® Low Density Array study". International Journal of Molecular Medicine 31.1 (2013): 129-137.
Chicago
Genda, Y., Arai, M., Ishikawa, M., Tanaka, S., Okabe, T., Sakamoto, A."microRNA changes in the dorsal horn of the spinal cord of rats with chronic constriction injury: A TaqMan® Low Density Array study". International Journal of Molecular Medicine 31, no. 1 (2013): 129-137. https://doi.org/10.3892/ijmm.2012.1163