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Article

The flavonoid glabridin attenuates 2-deoxy-D-ribose-induced oxidative damage and cellular dysfunction in MC3T3-E1 osteoblastic cells

  • Authors:
    • Hyun-Sook Kim
    • Kwang Sik Suh
    • Ara Ko
    • Donggeun Sul
    • Dalwoong  Choi
    • Seung Kwan Lee
    • Woon-Won Jung
  • View Affiliations / Copyright

    Affiliations: Department of Biomedical Science, College of Health Science, Korea University, Seongbuk-gu, Seoul 136‑703, Republic of Korea, Research Institute of Endocrinology, Kyung Hee University Hospital, Dondaemun-gu, Seoul 130‑702, Republic of Korea, Department of Biomedical Sciences, College of Medicine, Seongbuk-gu, Seoul 136-705, Republic of Korea, Environmental Toxico-Genomic and Proteomic Center, College of Medicine, Seongbuk-gu, Seoul 136-705, Republic of Korea, Department of Environmental Health, Korea University, Seongbuk-gu, Seoul 136-703, Seoul, Republic of Korea, Department of Biomedical Science, College of Health Science, Korea University, Seongbuk-gu, Seoul 136‑703, Republic of Korea, Research Institute of Health Science, College of Health Science, Korea University, Seongbuk-gu, Seoul 136-703, Seoul, Republic of Korea
  • Pages: 243-251
    |
    Published online on: November 2, 2012
       https://doi.org/10.3892/ijmm.2012.1172
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Abstract

Reducing sugar 2-deoxy-D-ribose (dRib) produces reactive oxygen species (ROS) through autoxidation and protein glycosylation and causes dysfunction of osteoblasts. In the present study, glabridin, a natural flavonoid, was investigated to determine whether it could influence dRib-induced oxidative damage and cellular dysfunction in the MC3T3-E1 mouse osteoblastic cell line. Osteoblastic cells were treated with dRib in the presence or absence of glabridin. Cell viability, apoptosis, ROS production and mitochondrial membrane potential (ΔΨm) were subsequently examined. It was observed that dRib reduced cell survival and ΔΨm, while it markedly increased intracellular levels of ROS and apoptosis. However, pretreatment of cells with glabridin attenuated all the dRib-induced effects. The antioxidant N-acetyl-L-cysteine (NAC) also prevented dRib-induced oxidative cell damage. In addition, treatment with glabridin resulted in a significant elevation of alkaline phosphatase (ALP) activity, collagen contents and osteoblast differentiation genes [ALP, collagen, osteopontin (OPN), osteoprotegerin (OPG) and osteocalcin (OC)] and bone morphogenetic protein (BMP) genes (BMP2, BMP4 and BMP7). In mechanistic studies of the antioxidative potential of glabridin, we found that glabridin activated dRib-induced decreased expression of phosphatidylinositol 3'-kinase (PI3K) and protein kinase B 2 (AKT2) genes, which are master regulators of survival-related signaling pathways. Glabridin also upregulated the gene expression of antioxidant enzymes, superoxide dismutase 1 (SOD1) and glutathione peroxidase 4 (GPX4), which were inhibited by dRib. Taken together, these results suggest that glabridin attenuates dRib-induced cell damage in osteoblastic cells and may be useful for the treatment of diabetes-related bone disease.
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Copy and paste a formatted citation
Spandidos Publications style
Kim H, Suh KS, Ko A, Sul D, Choi D, Lee SK and Jung W: The flavonoid glabridin attenuates 2-deoxy-D-ribose-induced oxidative damage and cellular dysfunction in MC3T3-E1 osteoblastic cells. Int J Mol Med 31: 243-251, 2013.
APA
Kim, H., Suh, K.S., Ko, A., Sul, D., Choi, D., Lee, S.K., & Jung, W. (2013). The flavonoid glabridin attenuates 2-deoxy-D-ribose-induced oxidative damage and cellular dysfunction in MC3T3-E1 osteoblastic cells. International Journal of Molecular Medicine, 31, 243-251. https://doi.org/10.3892/ijmm.2012.1172
MLA
Kim, H., Suh, K. S., Ko, A., Sul, D., Choi, D., Lee, S. K., Jung, W."The flavonoid glabridin attenuates 2-deoxy-D-ribose-induced oxidative damage and cellular dysfunction in MC3T3-E1 osteoblastic cells". International Journal of Molecular Medicine 31.1 (2013): 243-251.
Chicago
Kim, H., Suh, K. S., Ko, A., Sul, D., Choi, D., Lee, S. K., Jung, W."The flavonoid glabridin attenuates 2-deoxy-D-ribose-induced oxidative damage and cellular dysfunction in MC3T3-E1 osteoblastic cells". International Journal of Molecular Medicine 31, no. 1 (2013): 243-251. https://doi.org/10.3892/ijmm.2012.1172
Copy and paste a formatted citation
x
Spandidos Publications style
Kim H, Suh KS, Ko A, Sul D, Choi D, Lee SK and Jung W: The flavonoid glabridin attenuates 2-deoxy-D-ribose-induced oxidative damage and cellular dysfunction in MC3T3-E1 osteoblastic cells. Int J Mol Med 31: 243-251, 2013.
APA
Kim, H., Suh, K.S., Ko, A., Sul, D., Choi, D., Lee, S.K., & Jung, W. (2013). The flavonoid glabridin attenuates 2-deoxy-D-ribose-induced oxidative damage and cellular dysfunction in MC3T3-E1 osteoblastic cells. International Journal of Molecular Medicine, 31, 243-251. https://doi.org/10.3892/ijmm.2012.1172
MLA
Kim, H., Suh, K. S., Ko, A., Sul, D., Choi, D., Lee, S. K., Jung, W."The flavonoid glabridin attenuates 2-deoxy-D-ribose-induced oxidative damage and cellular dysfunction in MC3T3-E1 osteoblastic cells". International Journal of Molecular Medicine 31.1 (2013): 243-251.
Chicago
Kim, H., Suh, K. S., Ko, A., Sul, D., Choi, D., Lee, S. K., Jung, W."The flavonoid glabridin attenuates 2-deoxy-D-ribose-induced oxidative damage and cellular dysfunction in MC3T3-E1 osteoblastic cells". International Journal of Molecular Medicine 31, no. 1 (2013): 243-251. https://doi.org/10.3892/ijmm.2012.1172
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