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Article

Activated microglia provide a neuroprotective role by balancing glial cell-line derived neurotrophic factor and tumor necrosis factor-α secretion after subacute cerebral ischemia

  • Authors:
    • Jianping Wang
    • Zhitang Yang
    • Cong Liu
    • Yuanzheng Zhao
    • Yibing Chen
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, State Key Laboratory of Oncology in Southern China and Department of Experimental Research, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong 510060, P.R. China
  • Pages: 172-178
    |
    Published online on: November 13, 2012
       https://doi.org/10.3892/ijmm.2012.1179
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Abstract

Microglia are the major immune cells in the central nervous system and play a key role in brain injury pathology. However, the role of activated microglia after subacute cerebral ischemia (SCI) remains unknown. To address this issue, we established a permanent middle cerebral artery occlusion (pMCAO) rat model and treated pMCAO rats with N-(6-oxo-5,6-dihydro-phenanthridin-2-yl)-N,N-dimethylacetamide (PJ34) (an inhibitor of microglial activation), or with vehicle alone. Finally, we determined the differences between the PJ34-and vehicle-treated rats with respect to neurological deficits, infarct volume, neuronal loss and the expression of CD11b (a marker of microglial activation), glial cell line-derived neurotrophic factor (GDNF) and tumor necrosis factor-α (TNF-α) at 1, 3 and 7 days after treatment. We found that the PJ34-treated rats had more severe neurological deficits and a larger infarct volume and exhibited a decreased CD11b expression, more neuronal loss, decreased expression of GDNF mRNA and protein but increased expression of TNF-α mRNA and protein compared with the vehicle-treated rats at 3 and 7 days after treatment. These results indicate that activated microglia provide a neuroprotective role through balancing GDNF and TNF-α expression following SCI.
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Copy and paste a formatted citation
Spandidos Publications style
Wang J, Yang Z, Liu C, Zhao Y and Chen Y: Activated microglia provide a neuroprotective role by balancing glial cell-line derived neurotrophic factor and tumor necrosis factor-α secretion after subacute cerebral ischemia. Int J Mol Med 31: 172-178, 2013.
APA
Wang, J., Yang, Z., Liu, C., Zhao, Y., & Chen, Y. (2013). Activated microglia provide a neuroprotective role by balancing glial cell-line derived neurotrophic factor and tumor necrosis factor-α secretion after subacute cerebral ischemia. International Journal of Molecular Medicine, 31, 172-178. https://doi.org/10.3892/ijmm.2012.1179
MLA
Wang, J., Yang, Z., Liu, C., Zhao, Y., Chen, Y."Activated microglia provide a neuroprotective role by balancing glial cell-line derived neurotrophic factor and tumor necrosis factor-α secretion after subacute cerebral ischemia". International Journal of Molecular Medicine 31.1 (2013): 172-178.
Chicago
Wang, J., Yang, Z., Liu, C., Zhao, Y., Chen, Y."Activated microglia provide a neuroprotective role by balancing glial cell-line derived neurotrophic factor and tumor necrosis factor-α secretion after subacute cerebral ischemia". International Journal of Molecular Medicine 31, no. 1 (2013): 172-178. https://doi.org/10.3892/ijmm.2012.1179
Copy and paste a formatted citation
x
Spandidos Publications style
Wang J, Yang Z, Liu C, Zhao Y and Chen Y: Activated microglia provide a neuroprotective role by balancing glial cell-line derived neurotrophic factor and tumor necrosis factor-α secretion after subacute cerebral ischemia. Int J Mol Med 31: 172-178, 2013.
APA
Wang, J., Yang, Z., Liu, C., Zhao, Y., & Chen, Y. (2013). Activated microglia provide a neuroprotective role by balancing glial cell-line derived neurotrophic factor and tumor necrosis factor-α secretion after subacute cerebral ischemia. International Journal of Molecular Medicine, 31, 172-178. https://doi.org/10.3892/ijmm.2012.1179
MLA
Wang, J., Yang, Z., Liu, C., Zhao, Y., Chen, Y."Activated microglia provide a neuroprotective role by balancing glial cell-line derived neurotrophic factor and tumor necrosis factor-α secretion after subacute cerebral ischemia". International Journal of Molecular Medicine 31.1 (2013): 172-178.
Chicago
Wang, J., Yang, Z., Liu, C., Zhao, Y., Chen, Y."Activated microglia provide a neuroprotective role by balancing glial cell-line derived neurotrophic factor and tumor necrosis factor-α secretion after subacute cerebral ischemia". International Journal of Molecular Medicine 31, no. 1 (2013): 172-178. https://doi.org/10.3892/ijmm.2012.1179
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