Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
January 2013 Volume 31 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January 2013 Volume 31 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Induction of differentiation-specific miRNAs in TPA-induced myeloid leukemia cells through MEK/ERK activation

  • Authors:
    • Jing Wang
    • Lijun Liu
    • Lan Xie
    • Guangxin Xiang
    • Yuxiang Zhou
  • View Affiliations / Copyright

    Affiliations: Medical Systems Biology Research Center, School of Medicine, Tsinghua University, Beijing 100084, P.R. China, Reproductive Medicine Center, Τhe First Hospital of Lanzhou University, Lanzhou, Gansu 730000, P.R. China, National Engineering Research Center for Beijing Biochip Technology, Beijing 102206, P.R. China
  • Pages: 59-66
    |
    Published online on: November 21, 2012
       https://doi.org/10.3892/ijmm.2012.1191
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Cellular microRNAs (miRNAs) are pivotal regulators involved in various biological processes through the post-transcriptional regulation of gene expression. Signaling pathways are extensively activated during 12-O-Tetradecanoylphorbol-13-acetate (TPA)-induced differentiation of human leukemia cells, but the modulation of miRNA expression and processing in this context has yet to be fully explored. In this study, we comprehensively analyzed 10 miRNAs that are consistently upregulated during TPA-induced differentiation of various leukemia cell lines by employing microarray technology. The upregulation of these miRNAs was further verified by quantitative RT-PCR, and, markedly, a subset of the miRNAs was found to be induced via the MEK/ERK signaling pathway using TPA and specific pharmacological inhibitors. Moreover, immunoblotting and quantitative RT-PCR analysis demonstrated that the expression levels of key miRNA processing machineries (i.e., Drosha, Dicer, Ago1 and Ago2) were not induced in this context, but the transcription of the miRNA products was triggered by MEK/ERK activation. Therefore, we identified the unique miRNAs that respond to TPA treatment in leukemia cells and demonstrated the essential role of the MEK/ERK signaling pathway in the induction of these miRNA transcripts.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Kluiver J, Kroesen BJ, Poppema S and van den Berg A: The role of microRNAs in normal hematopoiesis and hematopoietic malignancies. Leukemia. 20:1931–1936. 2006. View Article : Google Scholar : PubMed/NCBI

2 

Nowak D, Stewart D and Koeffler HP: Differentiation therapy of leukemia: 3 decades of development. Blood. 113:3655–3665. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Schaar D, Goodell L, Aisner J, et al: A phase I clinical trial of 12- O-tetradecanoylphorbol-13-acetate for patients with relapsed/refractory malignancies. Cancer Chemother Pharmacol. 57:789–795. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Chang SH and Hla T: Gene regulation by RNA binding proteins and microRNAs in angiogenesis. Trends Mol Med. 17:650–658. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Kim VN, Han J and Siomi MC: Biogenesis of small RNAs in animals. Nat Rev Mol Cell Biol. 10:126–139. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Davis-Dusenbery BN and Hata A: Mechanisms of control of microRNA biogenesis. J Biochem. 148:381–392. 2010.

7 

Kasashima K, Nakamura Y and Kozu T: Altered expression profiles of microRNAs during TPA-induced differentiation of HL-60 cells. Biochem Biophys Res Commun. 322:403–410. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Lal A, Pan Y, Navarro F, et al: miR-24-mediated downregulation of H2AX suppresses DNA repair in terminally differentiated blood cells. Nat Struct Mol Biol. 16:492–498. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Forrest AR, Kanamori-Katayama M, Tomaru Y, et al: Induction of microRNAs, mir-155, mir-222, mir-424 and mir-503, promotes monocytic differentiation through combinatorial regulation. Leukemia. 24:460–466. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Wang XS and Zhang JW: The microRNAs involved in human myeloid differentiation and myelogenous/myeloblastic leukemia. J Cell Mol Med. 12:1445–1455. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Matsumoto E, Hatanaka M, Bohgaki M and Maeda S: PKC pathway and ERK/MAPK pathway are required for induction of cyclin D1 and p21Waf1 during 12-o-tetradecanoylphorbol 13-acetate-induced differentiation of myeloleukemia cells. Kobe J Med Sci. 52:181–194. 2006.PubMed/NCBI

12 

Schaar DG, Liu H, Sharma S, et al: 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced dual-specificity phosphatase expression and AML cell survival. Leuk Res. 29:1171–1179. 2005. View Article : Google Scholar : PubMed/NCBI

13 

Wang J, Xiang G, Mitchelson K and Zhou Y: Microarray profiling of monocytic differentiation reveals miRNA-mRNA intrinsic correlation. J Cell Biochem. 112:2443–2453. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Kim S, Lee HS, Lee SK, et al: 12-O-Tetradecanoyl phorbol-13-acetate (TPA)-induced growth arrest is increased by silibinin by the downregulation of cyclin B1 and cdc2 and the upregulation of p21 expression in MDA-MB231 human breast cancer cells. Phytomedicine. 17:1127–1132. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Park MH, Park SY and Kim Y: Induction of proline-rich tyrosine kinase2 (Pyk2) through C/EBPbeta is involved in PMA-induced monocyte differentiation. FEBS Lett. 582:415–422. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Guo Y, Chen Z, Zhang L, et al: Distinctive microRNA profiles relating to patient survival in esophageal squamous cell carcinoma. Cancer Res. 68:26–33. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Tusher VG, Tibshirani R and Chu G: Significance analysis of microarrays applied to the ionizing radiation response. Proc Natl Acad Sci USA. 98:5116–5121. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Chen C, Ridzon DA, Broomer AJ, et al: Real-time quantification of microRNAs by stem-loop RT-PCR. Nucleic Acids Res. 33:e1792005. View Article : Google Scholar : PubMed/NCBI

19 

Li X, Liu J, Zhou R, Huang S and Chen XM: Gene silencing of MIR22 in acute lymphoblastic leukaemia involves histone modifications independent of promoter DNA methylation. Br J Haematol. 148:69–79. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Heller G, Schmidt WM, Ziegler B, et al: Genome-wide transcriptional response to 5-aza-2′-deoxycytidine and trichostatin a in multiple myeloma cells. Cancer Res. 68:44–54. 2008.

21 

Cameron JE, Fewell C, Yin Q, et al: Epstein-Barr virus growth/latency III program alters cellular microRNA expression. Virology. 382:257–266. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Nagpal JK, Rani R, Trink B and Saini KS: Targeting miRNAs for drug discovery: a new paradigm. Curr Mol Med. 10:503–510. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Ichimura A, Ruike Y, Terasawa K and Tsujimoto G: miRNAs and regulation of cell signaling. FEBS J. 278:1610–1618. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Tsuchiya N, Izumiya M, Ogata-Kawata H, et al: Tumor suppressor miR-22 determines p53-dependent cellular fate through post-transcriptional regulation of p21. Cancer Res. 71:4628–4639. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Huang S, He X, Ding J, et al: Upregulation of miR-23a approximately 27a approximately 24 decreases transforming growth factor-beta-induced tumor-suppressive activities in human hepatocellular carcinoma cells. Int J Cancer. 123:972–978. 2008. View Article : Google Scholar

26 

Wang P, Zou F, Zhang X, et al: microRNA-21 negatively regulates Cdc25A and cell cycle progression in colon cancer cells. Cancer Res. 69:8157–8165. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Sayed D, Rane S, Lypowy J, et al: MicroRNA-21 targets Sprouty2 and promotes cellular outgrowths. Mol Biol Cell. 19:3272–3282. 2008. View Article : Google Scholar : PubMed/NCBI

28 

Zaidi SK, Dowdy CR, van Wijnen AJ, et al: Altered Runx1 subnuclear targeting enhances myeloid cell proliferation and blocks differentiation by activating a miR-24/MKP-7/MAPK network. Cancer Res. 69:8249–8255. 2009. View Article : Google Scholar : PubMed/NCBI

29 

Lin RJ, Lin YC, Chen J, et al: microRNA signature and expression of Dicer and Drosha can predict prognosis and delineate risk groups in neuroblastoma. Cancer Res. 70:7841–7850. 2010. View Article : Google Scholar : PubMed/NCBI

30 

Vaksman O, Hetland TE, Trope CG, Reich R and Davidson B: Argonaute, Dicer, and Drosha are upregulated along tumor progression in serous ovarian carcinoma. Hum Pathol. May 29–2012.(Epub ahead of print).

31 

Yan M, Huang HY, Wang T, et al: Dysregulated expression of dicer and drosha in breast cancer. Pathol Oncol Res. 18:343–348. 2012. View Article : Google Scholar : PubMed/NCBI

32 

Frezzetti D, De Menna M, Zoppoli P, et al: Upregulation of miR-21 by Ras in vivo and its role in tumor growth. Oncogene. 30:275–286. 2011. View Article : Google Scholar : PubMed/NCBI

33 

Takagi S, Nakajima M, Kida K, Yamaura Y, Fukami T and Yokoi T: MicroRNAs regulate human hepatocyte nuclear factor 4alpha, modulating the expression of metabolic enzymes and cell cycle. J Biol Chem. 285:4415–4422. 2010. View Article : Google Scholar : PubMed/NCBI

34 

Schmeier S, MacPherson CR, Essack M, et al: Deciphering the transcriptional circuitry of microRNA genes expressed during human monocytic differentiation. BMC Genomics. 10:5952009. View Article : Google Scholar : PubMed/NCBI

35 

Zhou R, Hu G, Gong AY and Chen XM: Binding of NF-kappaB p65 subunit to the promoter elements is involved in LPS-induced transactivation of miRNA genes in human biliary epithelial cells. Nucleic Acids Res. 38:3222–3232. 2010. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wang J, Liu L, Xie L, Xiang G and Zhou Y: Induction of differentiation-specific miRNAs in TPA-induced myeloid leukemia cells through MEK/ERK activation. Int J Mol Med 31: 59-66, 2013.
APA
Wang, J., Liu, L., Xie, L., Xiang, G., & Zhou, Y. (2013). Induction of differentiation-specific miRNAs in TPA-induced myeloid leukemia cells through MEK/ERK activation. International Journal of Molecular Medicine, 31, 59-66. https://doi.org/10.3892/ijmm.2012.1191
MLA
Wang, J., Liu, L., Xie, L., Xiang, G., Zhou, Y."Induction of differentiation-specific miRNAs in TPA-induced myeloid leukemia cells through MEK/ERK activation". International Journal of Molecular Medicine 31.1 (2013): 59-66.
Chicago
Wang, J., Liu, L., Xie, L., Xiang, G., Zhou, Y."Induction of differentiation-specific miRNAs in TPA-induced myeloid leukemia cells through MEK/ERK activation". International Journal of Molecular Medicine 31, no. 1 (2013): 59-66. https://doi.org/10.3892/ijmm.2012.1191
Copy and paste a formatted citation
x
Spandidos Publications style
Wang J, Liu L, Xie L, Xiang G and Zhou Y: Induction of differentiation-specific miRNAs in TPA-induced myeloid leukemia cells through MEK/ERK activation. Int J Mol Med 31: 59-66, 2013.
APA
Wang, J., Liu, L., Xie, L., Xiang, G., & Zhou, Y. (2013). Induction of differentiation-specific miRNAs in TPA-induced myeloid leukemia cells through MEK/ERK activation. International Journal of Molecular Medicine, 31, 59-66. https://doi.org/10.3892/ijmm.2012.1191
MLA
Wang, J., Liu, L., Xie, L., Xiang, G., Zhou, Y."Induction of differentiation-specific miRNAs in TPA-induced myeloid leukemia cells through MEK/ERK activation". International Journal of Molecular Medicine 31.1 (2013): 59-66.
Chicago
Wang, J., Liu, L., Xie, L., Xiang, G., Zhou, Y."Induction of differentiation-specific miRNAs in TPA-induced myeloid leukemia cells through MEK/ERK activation". International Journal of Molecular Medicine 31, no. 1 (2013): 59-66. https://doi.org/10.3892/ijmm.2012.1191
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team