Molecular cloning, characterization and differential expression of DRK1 in Sporothrix schenckii

Hou,Binbin; Zhang,Zhenying; Zheng,Fangliang; Liu,Xiaoming

doi:10.3892/ijmm.2012.1193

January 2013 Volume 31 Issue 1

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January 2013 Volume 31 Issue 1

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Article

Molecular cloning, characterization and differential expression of DRK1 in Sporothrix schenckii

Authors:
- Binbin Hou
- Zhenying Zhang
- Fangliang Zheng
- Xiaoming Liu
View Affiliations / Copyright

Affiliations: Department of Dermatology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China, Key Laboratory of Animal Resource and Epidemic Disease Prevention, Life Science School of Liaoning University, Shenyang, Liaoning 110036, P.R. China
Pages: 99-104
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Published online on: November 22, 2012

https://doi.org/10.3892/ijmm.2012.1193
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Abstract

The dimorphism of Sporothrix schenckii (S. schenckii) reflects a developmental switch in morphology and lifestyle that is necessary for virulence. DRK1, a hybrid histidine kinase, functions as a global regulator of dimorphism and virulence in Blastomyces dermatitidis (B. dermatitidis) and Histoplasma capsulatum (H. capsulatum). The partial cDNA sequence of DRK1 of S. schenckii, designated SsDRK1, was obtained using degenerate primers based on the conserved domain of the DRK1 of other fungi. The complete cDNA sequence of SsDRK1 was obtained by 5' and 3' RACE. The full-length cDNA is 4743 bp in size and has an open reading frame (ORF) of 4071 bp, encoding 1356 amino acid residues. The predicted molecular mass of SsDRK1 is 147.3 kDa with an estimated theoretical isoelectric point of 5.46. The deduced amino acid sequence of SsDRK1 shows 65% identity to that of B. dermatitidis. The SsDRK1 was predicted to be a soluble histidine kinase and to contain three parts: sensor domain, linker domain and functional domain. Quantitative real-time RT-PCR revealed that SsDRK1 was more highly expressed in the yeast stage compared with that in the mycelial stage, which indicated that the SsDRK1 may be involved in the dimorphic switch in S. schenckii.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Molecular cloning, characterization and differential expression of DRK1 in Sporothrix schenckii

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