Vaccination with embryonic stem cells generates effective antitumor immunity against ovarian cancer

Zhang,Zujuan; Chen,Xinhua; Chang,Xiaohong; Ye,Xue; Li,Yi; Cui,Heng

doi:10.3892/ijmm.2012.1195

January 2013 Volume 31 Issue 1

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January 2013 Volume 31 Issue 1

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Article

Vaccination with embryonic stem cells generates effective antitumor immunity against ovarian cancer

Authors:
- Zujuan Zhang
- Xinhua Chen
- Xiaohong Chang
- Xue Ye
- Yi Li
- Heng Cui
View Affiliations / Copyright

Affiliations: Gynecologic Oncology Center, Peking University People's Hospital, Beijing 100044, P.R. China
Pages: 147-153
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Published online on: November 22, 2012

https://doi.org/10.3892/ijmm.2012.1195
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Abstract

To date, only a few studies have suggested that human embryonic stem cells (hESCs) might effectively immunize against colon and lung cancer. The purpose of this study was to investigate the therapeutic potential of hESCs as a vaccine to induce widespread antitumor effects in different animal models and various types of cancer. C57BL/6 mice with ID8 ovarian cancer cell and Fischer 344 rats with NuTu-19 ovarian cancer cell models were used. Fifty-four mice were divided into six groups with nine mice in each group. Each mouse was immunized with pre-inactivated hESCs (H9) or mouse embryonic stem cells (mESCs; IVP-ES1) or ID8 or phosphate-buffered saline (PBS). Twenty-four rats were divided into four groups with six rats in each group, each rat immunized with pre-inactivated hESCs (H9) or NuTu-19 or PBS. After the vaccination, each mouse was challenged with live ID8 cells subcutaneously, and each rat was challenged with live NuTu-19 cells intraperitoneally. We discovered that vaccination of mice with the hESC line H9 and the mESC line IVP-ES1 generated consistent cellular and humoral immune responses against ID8 ovarian cancer. H9 and IVP-ES1 vaccinated mice obtained antitumor immune protection, and H9 vaccinated rats had the longest survival time and least distant metastases. No evidence of side-effects was observed. We also compared the immunogenicity against ovarian cancer between the hESC line, H9, and the mESC line, IVP-ES1, that derived from the inner cell mass in different species. We found that there were no significant differences between them. Furthermore, immunohistochemical staining revealed that several oncogenes and tumor suppressor genes, such as HER-2, C-myc, p53, and nm23, were expressed in H9, many of which were also shared by ovarian cancer. hESC vaccines can induce antitumor effects in two animal models and in ovarian cancer, indicating that the activity of the vaccine is universal, and, more importantly, it is safe.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Vaccination with embryonic stem cells generates effective antitumor immunity against ovarian cancer

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