Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
February 2013 Volume 31 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
February 2013 Volume 31 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Intracellular-delivery of a single-chain antibody against hepatitis B core protein via cell-penetrating peptide inhibits hepatitis B virus replication in vitro

  • Authors:
    • Yunhao Xun
    • Qingchun Pan
    • Zhenghao Tang
    • Xiaohua Chen
    • Yongsheng Yu
    • Min Xi
    • Guoqing Zang
  • View Affiliations / Copyright

    Affiliations: Department of Infectious Disease, The Sixth People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, P.R. China
  • Pages: 369-376
    |
    Published online on: December 12, 2012
       https://doi.org/10.3892/ijmm.2012.1210
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Assembly of nucleocapsids is an attractive target for novel anti-hepatitis B virus (HBV) agents, and intracellular single-chain variable fragment (scFv) antibodies against HBV core (HBc) protein are a class of potential alternatives for this purpose; however, their application is limited by the lack of a suitable means of delivery. Owing to the favorable performance of cytoplasmic transduction peptide (CTP) in cargo delivery in hepatocytes, we purified an anti-HBc scFv fused to CTP using a previous screened sequence by a prokaryotic expression system and evaluated its efficacy in the inhibition of HBV in vitro. Our results showed that cytoplasmic translocation of the previous anti-HBc scFv was achieved by CTP in HepG2.2.15 cells. Immunoprecipitation analysis indicated the fusion protein anti-HBc scFv-CTP interacted with its target antigen HBc, and negligible cytotoxicity was observed. Moreover, the anti-HBc scFv-CTP interfered with nucleocapsid assembly and markedly reduced both the supernatant HBV DNA level and the intracellular DNA replication intermediates, with a 5.1 µM of half maximal effect concentration and a dose-dependent effect. In conclusion, this novel anti-HBc scFv fused to CTP demonstrated inhibitory activity of HBV replication in vitro and warrants further in vivo study.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1. 

Zanetti AR, Van Damme P and Shouval D: The global impact of vaccination against hepatitis B: a historical overview. Vaccine. 26:6266–6273. 2008. View Article : Google Scholar : PubMed/NCBI

2. 

Perrillo R: Benefits and risks of interferon therapy for hepatitis B. Hepatology. 49(Suppl 5): S103–S111. 2009. View Article : Google Scholar : PubMed/NCBI

3. 

Dienstag JL: Benefits and risks of nucleoside analog therapy for hepatitis B. Hepatology. 49(Suppl 5): S112–S121. 2009. View Article : Google Scholar : PubMed/NCBI

4. 

Snow-Lampart A, Chappell B, Curtis M, et al: No resistance to tenofovir disoproxil fumarate detected after up to 144 weeks of therapy in patients monoinfected with chronic hepatitis B virus. Hepatology. 53:763–773. 2011. View Article : Google Scholar : PubMed/NCBI

5. 

Bingfa X, Qinglin F, Hui H, Canjun W, Wei W and Lihua S: Anti-hepatitis B virus activity and mechanisms of recombinant human serum albumin-interferon-alpha-2b fusion protein in vitro and in vivo. Pharmacology. 83:323–332. 2009. View Article : Google Scholar : PubMed/NCBI

6. 

Carey I and Harrison PM: Monotherapy versus combination therapy for the treatment of chronic hepatitis B. Expert Opin Investig Drugs. 18:1655–1666. 2009. View Article : Google Scholar : PubMed/NCBI

7. 

Urban S, Schulze A, Dandri M and Petersen J: The replication cycle of hepatitis B virus. J Hepatol. 52:282–284. 2010. View Article : Google Scholar : PubMed/NCBI

8. 

Stein LL and Loomba R: Drug targets in hepatitis B virus infection. Infect Disord Drug Targets. 9:105–116. 2009. View Article : Google Scholar : PubMed/NCBI

9. 

Grimm D, Thimme R and Blum HE: HBV life cycle and novel drug targets. Hepatol Int. 5:644–653. 2011. View Article : Google Scholar : PubMed/NCBI

10. 

Levrero M, Pollicino T, Petersen J, Belloni L, Raimondo G and Dandri M: Control of cccDNA function in hepatitis B virus infection. J Hepatol. 51:581–592. 2009. View Article : Google Scholar : PubMed/NCBI

11. 

Katen SP, Chirapu SR, Finn MG and Zlotnick A: Trapping of hepatitis B virus capsid assembly intermediates by phenylpropenamide assembly accelerators. ACS Chem Biol. 5:1125–1136. 2010. View Article : Google Scholar : PubMed/NCBI

12. 

Deres K, Schroder CH, Paessens A, et al: Inhibition of hepatitis B virus replication by drug-induced depletion of nucleocapsids. Science. 299:893–896. 2003. View Article : Google Scholar : PubMed/NCBI

13. 

Shi C, Wu CQ, Cao AM, Sheng HZ, Yan XZ and Liao MY: NMR-spectroscopy-based metabonomic approach to the analysis of Bay41-4109, a novel anti-HBV compound, induced hepatotoxicity in rats. Toxicol Lett. 173:161–167. 2007. View Article : Google Scholar : PubMed/NCBI

14. 

Han J, Pan X, Gao Y and Weil L: Inhibition of hepatitis B virus replication by the internal fragment of hepatitis B core protein. Virus Res. 150:129–134. 2010. View Article : Google Scholar : PubMed/NCBI

15. 

Yamamoto M, Hayashi N, Takehara T, et al: Intracellular single-chain antibody against hepatitis B virus core protein inhibits the replication of hepatitis B virus in cultured cells. Hepatology. 30:300–307. 1999. View Article : Google Scholar : PubMed/NCBI

16. 

Jin YH, Hong SH, Kim K, Shin HJ and Park S: Intracellular antibody fragment against hepatitis B virus X protein does not inhibit viral replication. Yonsei Med J. 47:721–728. 2006. View Article : Google Scholar : PubMed/NCBI

17. 

Heng BC and Cao T: Making cell-permeable recombinant telomerase (trans-telomerase) through fusion of its catalytic subunit (hTERT) with protein transduction domains (PTD): a possible strategy to overcome replicative senescence during ex vivo culture of primary explanted cells. Med Hypotheses. 65:199–200. 2005.

18. 

Gump JM and Dowdy SF: TAT transduction: the molecular mechanism and therapeutic prospects. Trends Mol Med. 13:443–448. 2007. View Article : Google Scholar : PubMed/NCBI

19. 

Zorko M and Langel U: Cell-penetrating peptides: mechanism and kinetics of cargo delivery. Adv Drug Deliv Rev. 57:529–545. 2005. View Article : Google Scholar : PubMed/NCBI

20. 

Doorbar J and Griffin H: Intrabody strategies for the treatment of human papillomavirus-associated disease. Expert Opin Biol Ther. 7:677–689. 2007. View Article : Google Scholar : PubMed/NCBI

21. 

Fonseca SB, Pereira MP and Kelley SO: Recent advances in the use of cell-penetrating peptides for medical and biological applications. Adv Drug Deliv Rev. 61:953–964. 2009. View Article : Google Scholar : PubMed/NCBI

22. 

Chames P, Van Regenmortel M, Weiss E and Baty D: Therapeutic antibodies: successes, limitations and hopes for the future. Br J Pharmacol. 157:220–233. 2009. View Article : Google Scholar : PubMed/NCBI

23. 

Pan XB, Wei L, Han JC, Ma H, Deng K and Cong X: Artificial recombinant cell-penetrating peptides interfere with envelopment of hepatitis B virus nucleocapsid and viral production. Antiviral Res. 89:109–114. 2011. View Article : Google Scholar : PubMed/NCBI

24. 

Tang ZH, Ma HH, Chen WS, Gu L, Li G and Yao JL: Screening, identifying and sequencing of human single-chain variable fragment specific to hepatitis B virus core protein. Zhongguo Binglishengli Zazhi. 19:329–333. 2003.(In Chinese).

25. 

Kim D, Jeon C, Kim JH, et al: Cytoplasmic transduction peptide (CTP): new approach for the delivery of biomolecules into cytoplasm in vitro and in vivo. Exp Cell Res. 312:1277–1288. 2006. View Article : Google Scholar : PubMed/NCBI

26. 

Friguet B, Chaffotte AF, Djavadi-Ohaniance L and Goldberg ME: Measurements of the true affinity constant in solution of antigen-antibody complexes by enzyme-linked immunosorbent assay. J Immunol Methods. 77:305–319. 1985. View Article : Google Scholar : PubMed/NCBI

27. 

Butz K, Denk C, Fitscher B, et al: Peptide aptamers targeting the hepatitis B virus core protein: a new class of molecules with antiviral activity. Oncogene. 20:6579–6586. 2001. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Xun Y, Pan Q, Tang Z, Chen X, Yu Y, Xi M and Zang G: Intracellular-delivery of a single-chain antibody against hepatitis B core protein via cell-penetrating peptide inhibits hepatitis B virus replication in vitro. Int J Mol Med 31: 369-376, 2013.
APA
Xun, Y., Pan, Q., Tang, Z., Chen, X., Yu, Y., Xi, M., & Zang, G. (2013). Intracellular-delivery of a single-chain antibody against hepatitis B core protein via cell-penetrating peptide inhibits hepatitis B virus replication in vitro. International Journal of Molecular Medicine, 31, 369-376. https://doi.org/10.3892/ijmm.2012.1210
MLA
Xun, Y., Pan, Q., Tang, Z., Chen, X., Yu, Y., Xi, M., Zang, G."Intracellular-delivery of a single-chain antibody against hepatitis B core protein via cell-penetrating peptide inhibits hepatitis B virus replication in vitro". International Journal of Molecular Medicine 31.2 (2013): 369-376.
Chicago
Xun, Y., Pan, Q., Tang, Z., Chen, X., Yu, Y., Xi, M., Zang, G."Intracellular-delivery of a single-chain antibody against hepatitis B core protein via cell-penetrating peptide inhibits hepatitis B virus replication in vitro". International Journal of Molecular Medicine 31, no. 2 (2013): 369-376. https://doi.org/10.3892/ijmm.2012.1210
Copy and paste a formatted citation
x
Spandidos Publications style
Xun Y, Pan Q, Tang Z, Chen X, Yu Y, Xi M and Zang G: Intracellular-delivery of a single-chain antibody against hepatitis B core protein via cell-penetrating peptide inhibits hepatitis B virus replication in vitro. Int J Mol Med 31: 369-376, 2013.
APA
Xun, Y., Pan, Q., Tang, Z., Chen, X., Yu, Y., Xi, M., & Zang, G. (2013). Intracellular-delivery of a single-chain antibody against hepatitis B core protein via cell-penetrating peptide inhibits hepatitis B virus replication in vitro. International Journal of Molecular Medicine, 31, 369-376. https://doi.org/10.3892/ijmm.2012.1210
MLA
Xun, Y., Pan, Q., Tang, Z., Chen, X., Yu, Y., Xi, M., Zang, G."Intracellular-delivery of a single-chain antibody against hepatitis B core protein via cell-penetrating peptide inhibits hepatitis B virus replication in vitro". International Journal of Molecular Medicine 31.2 (2013): 369-376.
Chicago
Xun, Y., Pan, Q., Tang, Z., Chen, X., Yu, Y., Xi, M., Zang, G."Intracellular-delivery of a single-chain antibody against hepatitis B core protein via cell-penetrating peptide inhibits hepatitis B virus replication in vitro". International Journal of Molecular Medicine 31, no. 2 (2013): 369-376. https://doi.org/10.3892/ijmm.2012.1210
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team