Inhibitory effects of paeoniflorin on lysophosphatidylcholine-induced inflammatory factor production in human umbilical vein endothelial cells

  • Authors:
    • Jian-Zhe Li
    • Jian-Hua Wu
    • Shu-Yi Yu
    • Qing-Rui Shao
    • Xiao-Min Dong
  • View Affiliations

  • Published online on: December 13, 2012     https://doi.org/10.3892/ijmm.2012.1211
  • Pages: 493-497
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Lysophosphatidylcholine (LPC) plays an important role in atherosclerosis through initiation of endothelial inflammation response. Paeoniflorin (PEF), isolated from the dry root of Paeonia, has been reported to exert an anti-inflammatory effect, but the exact mechanism is not fully understood. The aim of this study was to investigate the inhibitory effects of PEF on LPC-induced inflammatory factor production and the underlying mechanisms. In human umbilical vein endothelial cells (HUVECs), different concentrations (1, 10 or 100 µmol/l) of PEF were added 2 h prior to exposure to LPC (10 mg/l) for 24 h. The results showed that PEF significantly inhibited LPC-induced inflammatory factor production. In addition, PEF was also able to suppress the enhanced high mobility group box-1 (HMGB1) expression and release, upregulated expression of receptor for advanced glycation end product (RAGE), Toll-like receptor (TLR)-2 and TLR-4, and increased nuclear factor-κB (NF-κB) activity induced by LPC. Our results suggest that PEF suppresses LPC-induced inflammatory factor production through inhibition of the HMGB1-RAGE/TLR-2/TLR-4-NF-κB pathway.
View Figures
View References

Related Articles

Journal Cover

February 2013
Volume 31 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Li J, Wu J, Yu S, Shao Q and Dong X: Inhibitory effects of paeoniflorin on lysophosphatidylcholine-induced inflammatory factor production in human umbilical vein endothelial cells. Int J Mol Med 31: 493-497, 2013
APA
Li, J., Wu, J., Yu, S., Shao, Q., & Dong, X. (2013). Inhibitory effects of paeoniflorin on lysophosphatidylcholine-induced inflammatory factor production in human umbilical vein endothelial cells. International Journal of Molecular Medicine, 31, 493-497. https://doi.org/10.3892/ijmm.2012.1211
MLA
Li, J., Wu, J., Yu, S., Shao, Q., Dong, X."Inhibitory effects of paeoniflorin on lysophosphatidylcholine-induced inflammatory factor production in human umbilical vein endothelial cells". International Journal of Molecular Medicine 31.2 (2013): 493-497.
Chicago
Li, J., Wu, J., Yu, S., Shao, Q., Dong, X."Inhibitory effects of paeoniflorin on lysophosphatidylcholine-induced inflammatory factor production in human umbilical vein endothelial cells". International Journal of Molecular Medicine 31, no. 2 (2013): 493-497. https://doi.org/10.3892/ijmm.2012.1211