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B7-H3 overexpression in pancreatic cancer promotes tumor progression

  • Authors:
    • Xin Zhao
    • De-Chun Li
    • Xin-Guo Zhu
    • Wen-Juan Gan
    • Zhi Li
    • Feng Xiong
    • Zi-Xiang Zhang
    • Guang-Bo Zhang
    • Xue-Guang Zhang
    • Hua Zhao
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, P.R. China, Department of Pathology, The First Affiliated Hospital of Soochow University, Suzhou, P.R. China, Department of Interventional Radiology, The First Affiliated Hospital of Soochow University, Suzhou, P.R. China, Department of Oncology, The First Affiliated Hospital of Soochow University, Suzhou, P.R. China, Department of Clinical Immunology, The First Affiliated Hospital of Soochow University, Suzhou, P.R. China
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 283-291
    |
    Published online on: December 13, 2012
       https://doi.org/10.3892/ijmm.2012.1212
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Abstract

B7-H3, a member of the B7-family molecules, plays an important role in adaptive immune responses. In addition, B7-H3 is also expressed in several types of human cancers and is correlated with the poor outcome of cancer patients. However, its exact role in cancer is not known. In the present study, we compared B7-H3 expression in normal pancreas and pancreatic cancer tissue specimens, and determined the effects of low B7-H3 expression on the human pancreatic cancer cell line Patu8988 using lentivirus-mediated RNA interference. B7-H3 expression in pancreatic specimens was determined by enzyme-linked immunosorbent assay (ELISA). A Patu8988 cell line with low B7-H3 expression was established by lentivirus-mediated RNA interference to investigate the effect of B7-H3 on cell proliferation, migration and invasion in vitro. By establishing subcutaneous transplantation tumor and orthotopic transplantation pancreatic cancer mouse models, the effect of B7-H3 on cell proliferation, migration and invasion was studied in vivo. B7-H3 in tissue samples was significantly higher in the pancreatic cancer group than in the normal pancreas group (mean ± SD, 193.6±9.352 vs. 87.74±7.433 ng/g; P<0.0001). B7-H3 knockdown by RNA interference decreased cell migration and Transwell invasion up to 50% in vitro. No apparent impact was observed on cell proliferation in vitro. In the subcutaneous transplantation tumor mouse model, the tumor growth rate was reduced by the knockdown of B7-H3. In the orthotopic transplantation pancreatic cancer mouse model, the effect of inhibiting metastasis by knocking down B7-H3 was assessed in terms of the average postmortem abdominal visceral metastatic tumor weight. This demonstrated that inhibition of B7-H3 expression reduced pancreatic cancer metastasis in vivo. In conclusion, B7-H3 is aberrantly expressed in pancreatic cancer. In addition to modulating tumor immunity, B7-H3 may have a novel role in regulating pancreatic tumor progression.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao X, Li D, Zhu X, Gan W, Li Z, Xiong F, Zhang Z, Zhang G, Zhang X, Zhao H, Zhao H, et al: B7-H3 overexpression in pancreatic cancer promotes tumor progression. Int J Mol Med 31: 283-291, 2013.
APA
Zhao, X., Li, D., Zhu, X., Gan, W., Li, Z., Xiong, F. ... Zhao, H. (2013). B7-H3 overexpression in pancreatic cancer promotes tumor progression. International Journal of Molecular Medicine, 31, 283-291. https://doi.org/10.3892/ijmm.2012.1212
MLA
Zhao, X., Li, D., Zhu, X., Gan, W., Li, Z., Xiong, F., Zhang, Z., Zhang, G., Zhang, X., Zhao, H."B7-H3 overexpression in pancreatic cancer promotes tumor progression". International Journal of Molecular Medicine 31.2 (2013): 283-291.
Chicago
Zhao, X., Li, D., Zhu, X., Gan, W., Li, Z., Xiong, F., Zhang, Z., Zhang, G., Zhang, X., Zhao, H."B7-H3 overexpression in pancreatic cancer promotes tumor progression". International Journal of Molecular Medicine 31, no. 2 (2013): 283-291. https://doi.org/10.3892/ijmm.2012.1212
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao X, Li D, Zhu X, Gan W, Li Z, Xiong F, Zhang Z, Zhang G, Zhang X, Zhao H, Zhao H, et al: B7-H3 overexpression in pancreatic cancer promotes tumor progression. Int J Mol Med 31: 283-291, 2013.
APA
Zhao, X., Li, D., Zhu, X., Gan, W., Li, Z., Xiong, F. ... Zhao, H. (2013). B7-H3 overexpression in pancreatic cancer promotes tumor progression. International Journal of Molecular Medicine, 31, 283-291. https://doi.org/10.3892/ijmm.2012.1212
MLA
Zhao, X., Li, D., Zhu, X., Gan, W., Li, Z., Xiong, F., Zhang, Z., Zhang, G., Zhang, X., Zhao, H."B7-H3 overexpression in pancreatic cancer promotes tumor progression". International Journal of Molecular Medicine 31.2 (2013): 283-291.
Chicago
Zhao, X., Li, D., Zhu, X., Gan, W., Li, Z., Xiong, F., Zhang, Z., Zhang, G., Zhang, X., Zhao, H."B7-H3 overexpression in pancreatic cancer promotes tumor progression". International Journal of Molecular Medicine 31, no. 2 (2013): 283-291. https://doi.org/10.3892/ijmm.2012.1212
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