Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
April 2012 Volume 29 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
April 2012 Volume 29 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Resveratrol, a polyphenol phytoalexin, protects cardiomyocytes against anoxia/reoxygenation injury via the TLR4/NF-κB signaling pathway

  • Authors:
    • Cui Zhang
    • Guosheng Lin
    • Weiguo Wan
    • Xuyon Li
    • Bin Zeng
    • Bo Yang
    • Congxin Huang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Renmin Hospital of Wuhan University and Cardiovascular Research Institute of Wuhan University, Wuhan, P.R. China, Department of Cardiology, Renmin Hospital of Wuhan University and Cardiovascular Research Institute of Wuhan University, 238 Jiefang Road, Wuchang, Wuhan 430060, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 557-563
    |
    Published online on: January 11, 2012
       https://doi.org/10.3892/ijmm.2012.885
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Previous studies indicate resveratrol pretreatment can protect cardiomyocytes. However, it is largely unknown whether resveratrol protects cardiomyocytes when applied at reperfusion. The purpose of this study was to investigate whether resveratrol given at reoxygenation could protect cardiomyocytes under the anoxia/reoxygenation (A/R) condition and to examine the underlying mechanism. In this study, primary cultures of neonatal rat cardiomyocytes were randomly distributed into three groups: control group, A/R group (cultured cardiomyocytes were subjected to 3 h anoxia followed by 2 h reoxygenation), and the resveratrol group (cardiomyocytes were subjected to 3 h anoxia/2 h reoxygenation, and 5, 10 or 20 µM resveratrol was applied 5 min after reoxygenation). In order to evaluate cardiomyocyte damage, cell viability, lactate dehydrogenase (LDH) release, caspase-3 activity, and apoptosis were analyzed by the cell counting kit (CCK)-8 assay, colorimetric method and flow cytometry, respectively. The mRNA and protein expression of Toll-like receptor 4 (TLR4) were detected by quantitative real-time PCR and western blot analysis. Nuclear factor-κB (NF-κB) p65 protein and I-κBα protein levels were also examined by western blot analysis. The levels of proinflammatory cytokines in the culture medium were assessed by enzyme-linked immunosorbent assay. We found that resveratrol prevented a reduction in cell viability, decreased the amount of LDH release, attenuated apoptotic cells and decreased caspase-3 activity induced by A/R in cardiomyocytes. Furthermore, resveratrol treatment significantly attenuated the TLR4 expression, inhibited NF-κB activation and reduced the levels of tumor necrosis factor (TNF)-α and interleukin (IL)-1β caused by A/R injury in the culture medium. Treatment with resveratrol shortly after the onset of reoxygenation improves cell survival and attenuates A/R-induced inflammatory response. This protection mechanism is possibly related to the TLR4/NF-κB signaling pathway.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

E BraunwaldRA KlonerMyocardial reperfusion: a double-edged sword?J Clin Invest7617131719198510.1172/JCI1121604056048

2 

DM YellonDJ HausenloyMyocardial reperfusion injuryN Engl J Med35711211135200710.1056/NEJMra07166717855673

3 

X LvJ WanJ YangCytochrome P450 omega-hydroxylase inhibition reduces cardiomyocyte apoptosis via activation of ERK1/2 signaling in rat myocardial ischemia-reperfusionEur J Pharmacol596118126200810.1016/j.ejphar.2008.08.00818771665

4 

ML EntmanL MichaelRD RossenInflammation in the course of early myocardial ischemiaFASEB J52529253719911868978

5 

S FrantzL KobzikYD KimToll4 (TLR4) expression in cardiac myocytes in normal and failing myocardiumJ Clin Invest104271280199910.1172/JCI670910430608

6 

LC HsuJM ParkK ZhangThe protein kinase PKR is required for macrophage apoptosis after activation of Toll-like receptor 4Nature428341345200410.1038/nature0240515029200

7 

DY JungH LeeBY JungTLR4, but not TLR2, signals autoregulatory apoptosis of cultured microglia: a critical role of IFN-beta as a decision makerJ Immunol17464676476200510.4049/jimmunol.174.10.646715879150

8 

SC KimJP SticeL ChenExtracellular heat shock protein 60, cardiac myocytes, and apoptosisCirc Res10511861195200910.1161/CIRCRESAHA.109.20964319875724

9 

K MiyakeInnate immune sensing of pathogens and danger signals by cell surface Toll-like receptorsSemin Immunol19310200710.1016/j.smim.2006.12.00217275324

10 

KJ HanX SuLG XuLH BinJ ZhangHB ShuMechanisms of the TRIF-induced interferon-stimulated response element and NF-kappaB activation and apoptosis pathwaysJ Biol Chem2791565215661200410.1074/jbc.M31162920014739303

11 

G BaumgartenP KnuefermannN NozakiN SivasubramanianDL MannJG VallejoIn vivo expression of proinflammatory mediators in the adult heart after endotoxin administration: the role of toll-like receptor-4J Infect Dis18316171624200110.1086/32071211343210

12 

J OyamaC Blais JrX LiuReduced myocardial ischemia-reperfusion injury in toll-like receptor 4-deficient miceCirculation109784789200410.1161/01.CIR.0000112575.66565.8414970116

13 

JA BaurDA SinclairTherapeutic potential of resveratrol: the in vivo evidenceNat Rev Drug Discov5493506200610.1038/nrd206016732220

14 

MY ShenG HsiaoCL LiuInhibitory mechanisms of resveratrol in platelet activation: pivotal roles of p38 MAPK and NO/cyclic GMPBr J Haematol139475485200717868048

15 

JG WoodB RoginaS LavuSirtuin activators mimic caloric restriction and delay ageing in metazoansNature430686689200410.1038/nature0278915254550

16 

PM BritoNF SimoesLM AlmeidaTC DinisResveratrol disrupts peroxynitrite-triggered mitochondrial apoptotic pathway: a role for Bcl-2Apoptosis1310431053200810.1007/s10495-008-0235-418584328

17 

G LanzilliA CottarelliG NicoteraS GuidaG RavagnanMP FuggettaAnti-inflammatory effect of resveratrol and polydatin by in vitro IL-17 modulationInflammationMar 32011(Epub ahead of print)

18 

S BradamanteL BarenghiF PiccininiResveratrol provides late-phase cardioprotection by means of a nitric oxide- and adenosine-mediated mechanismEur J Pharmacol465115123200310.1016/S0014-2999(03)01441-912650840

19 

S DasGA CordisN MaulikDK DasPharmacological preconditioning with resveratrol: role of CREB-dependent Bcl-2 signaling via adenosine A3 receptor activationAm J Physiol Heart Circ Physiol288H328H335200510.1152/ajpheart.00453.200415345477

20 

HS YounJY LeeKA FitzgeraldHA YoungS AkiraDH HwangSpecific inhibition of MyD88-independent signaling pathways of TLR3 and TLR4 by resveratrol: molecular targets are TBK1 and RIP1 in TRIF complexJ Immunol17533393346200510.4049/jimmunol.175.5.333916116226

21 

T RuiQ FengM LeiErythropoietin prevents the acute myocardial inflammatory response induced by ischemia/reperfusion via induction of AP-1Cardiovasc Res65719727200510.1016/j.cardiores.2004.11.01915664399

22 

HP ChenM HeQR HuangD LiuM HuangSasanquasaponin protects rat cardiomyocytes against oxidative stress induced by anoxia-reoxygenation injuryEur J Pharmacol5752127200710.1016/j.ejphar.2007.07.04317761161

23 

H ReineckeM ZhangT BartosekCE MurrySurvival, integration, and differentiation of cardiomyocyte grafts: a study in normal and injured rat heartsCirculation100193202199910.1161/01.CIR.100.2.19310402450

24 

T KoyamaK TemmaT AkeraReperfusion-induced contracture develops with a decreasing [Ca2+]i in single heart cellsAm J Physiol261H1115H112219911928393

25 

TM ScarabelliA StephanouE PasiniDifferent signaling pathways induce apoptosis in endothelial cells and cardiac myocytes during ischemia/reperfusion injuryCirc Res90745748200210.1161/01.RES.0000015224.07870.9A

26 

N MoorjaniS WestabyJ NarulaEffects of left ventricular volume overload on mitochondrial and death-receptor-mediated apoptotic pathways in the transition to heart failureAm J Cardiol10312611268200919406269

27 

TV ArumugamSL ChanDG JoGamma secretase-mediated Notch signaling worsens brain damage and functional outcome in ischemic strokeNat Med12621623200610.1038/nm140316680150

28 

W ChaoToll-like receptor signaling: a critical modulator of cell survival and ischemic injury in the heartAm J Physiol Heart Circ Physiol296H1H12200910.1152/ajpheart.00995.200819011041

29 

J ChaZ WangL AoCytokines link Toll-like receptor 4 signaling to cardiac dysfunction after global myocardial ischemiaAnn Thorac Surg8516781685200810.1016/j.athoracsur.2008.01.04318442564

30 

AJ ChongA ShimamotoCR HamptonToll-like receptor 4 mediates ischemia/reperfusion injury of the heartJ Thorac Cardiovasc Surg128170179200410.1016/j.jtcvs.2003.11.03615282452

31 

DJ KaczorowskiA NakaoR VallabhaneniMechanisms of Toll-like receptor 4 (TLR4)-mediated inflammation after cold ischemia/reperfusion in the heartTransplantation8714551463200910.1097/TP.0b013e3181a36e5e19461481

32 

Y WangAM AbarbanellJL HerrmannToll-like receptor signaling pathways and the evidence linking toll-like receptor signaling to cardiac ischemia/reperfusion injuryShock34548557201010.1097/SHK.0b013e3181e686f520458266

33 

RJ CarmodyYH ChenNuclear factor-kappaB: activation and regulation during toll-like receptor signalingCell Mol Immunol43141200717349209

34 

B SalaunP RomeroS LebecqueToll-like receptors’ two-edged sword: when immunity meets apoptosisEur J Immunol37331133182007

35 

A ShimamotoAJ ChongM YadaInhibition of Toll-like receptor 4 with eritoran attenuates myocardial ischemia-reperfusion injuryCirculation114Suppl 1S270274200610.1161/CIRCULATIONAHA.105.00090116820585

36 

J DudleyS DasS MukherjeeDK DasResveratrol, a unique phytoalexin present in red wine, delivers either survival signal or death signal to the ischemic myocardium depending on doseJ Nutr Biochem20443452200910.1016/j.jnutbio.2008.05.003

37 

S MukherjeeJI DudleyDK DasDose-dependency of resveratrol in providing health benefitsDose Response8478500201010.2203/dose-response.09-015.Mukherjee21191486

38 

DK DasN MaulikResveratrol in cardioprotection: a therapeutic promise of alternative medicineMol Interv63647200610.1124/mi.6.1.716507749

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhang C, Lin G, Wan W, Li X, Zeng B, Yang B and Huang C: Resveratrol, a polyphenol phytoalexin, protects cardiomyocytes against anoxia/reoxygenation injury via the TLR4/NF-κB signaling pathway. Int J Mol Med 29: 557-563, 2012.
APA
Zhang, C., Lin, G., Wan, W., Li, X., Zeng, B., Yang, B., & Huang, C. (2012). Resveratrol, a polyphenol phytoalexin, protects cardiomyocytes against anoxia/reoxygenation injury via the TLR4/NF-κB signaling pathway. International Journal of Molecular Medicine, 29, 557-563. https://doi.org/10.3892/ijmm.2012.885
MLA
Zhang, C., Lin, G., Wan, W., Li, X., Zeng, B., Yang, B., Huang, C."Resveratrol, a polyphenol phytoalexin, protects cardiomyocytes against anoxia/reoxygenation injury via the TLR4/NF-κB signaling pathway". International Journal of Molecular Medicine 29.4 (2012): 557-563.
Chicago
Zhang, C., Lin, G., Wan, W., Li, X., Zeng, B., Yang, B., Huang, C."Resveratrol, a polyphenol phytoalexin, protects cardiomyocytes against anoxia/reoxygenation injury via the TLR4/NF-κB signaling pathway". International Journal of Molecular Medicine 29, no. 4 (2012): 557-563. https://doi.org/10.3892/ijmm.2012.885
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang C, Lin G, Wan W, Li X, Zeng B, Yang B and Huang C: Resveratrol, a polyphenol phytoalexin, protects cardiomyocytes against anoxia/reoxygenation injury via the TLR4/NF-κB signaling pathway. Int J Mol Med 29: 557-563, 2012.
APA
Zhang, C., Lin, G., Wan, W., Li, X., Zeng, B., Yang, B., & Huang, C. (2012). Resveratrol, a polyphenol phytoalexin, protects cardiomyocytes against anoxia/reoxygenation injury via the TLR4/NF-κB signaling pathway. International Journal of Molecular Medicine, 29, 557-563. https://doi.org/10.3892/ijmm.2012.885
MLA
Zhang, C., Lin, G., Wan, W., Li, X., Zeng, B., Yang, B., Huang, C."Resveratrol, a polyphenol phytoalexin, protects cardiomyocytes against anoxia/reoxygenation injury via the TLR4/NF-κB signaling pathway". International Journal of Molecular Medicine 29.4 (2012): 557-563.
Chicago
Zhang, C., Lin, G., Wan, W., Li, X., Zeng, B., Yang, B., Huang, C."Resveratrol, a polyphenol phytoalexin, protects cardiomyocytes against anoxia/reoxygenation injury via the TLR4/NF-κB signaling pathway". International Journal of Molecular Medicine 29, no. 4 (2012): 557-563. https://doi.org/10.3892/ijmm.2012.885
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team