Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
April 2012 Volume 29 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
April 2012 Volume 29 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

The regulatory role of microRNA-1187 in TNF-α-mediated hepatocyte apoptosis in acute liver failure

  • Authors:
    • Dong Shan Yu
    • Fang Mei An
    • Bang Dong Gong
    • Xiao Gang Xiang
    • Lan Yi Lin
    • Hui Wang
    • Qing Xie
  • View Affiliations / Copyright

    Affiliations: Department of Infectious Disease, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, P.R. China, Department of Infectious Disease, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Ruijin er Road, no. 197, Shanghai 200025, P.R. China
  • Pages: 663-668
    |
    Published online on: January 17, 2012
       https://doi.org/10.3892/ijmm.2012.888
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

In the current study, we aimed at elucidating the regulatory mechanisms through which microR-1187 (miR-1187) participates in hepatocyte apoptosis in acute liver failure (ALF). An ALF model was induced with D-galactosamine (D-GalN) plus lipopolysaccharide (LPS) in BALB/c mice. The hepatic miRNA expression profile was detected by microarray analysis and verified by quantitative real-time PCR (qRT-PCR). The possible underlying mechanism was investigated in vitro using an embryonic murine hepatocyte cell line (BNLCL2) and miR-1187 mimic. Caspase-8 protein was detected by Western blotting and cell apoptosis was assayed by flow cytometry. Hepatic miR-1187 was down-regulated in ALF mice based on microarray data (P<0.001) and verified by qRT-PCR (P<0.01). Target scan revealed that caspase-8 was the putative target of miR-1187. In an in vitro study, miR-1187 showed the highest up-regulation in BNLCL2 cells transfected with the miR-1187 mimic at a 50 nM concentration for 12 h compared with cells transfected with the non-specific mimic (P<0.001). miR-1187 was down-regulated (P<0.01) but caspase-8 mRNA (P<0.01) as well as protein (P<0.05) were up-regulated in the BNLCL2 cells treated with D-GalN/TNF. Furthermore, overexpressed miR-1187 reduced caspase-8 expression at both the mRNA and protein levels significantly (P<0.01 and P<0.05 respectively), and significantly attenuated the apoptotic rate of BNLCL2 cells (P<0.05). We show that miR-1187 regulates hepatocyte apoptosis by targeting caspase-8. miR-1187 may serve as a potential therapeutic target for the treatment of ALF.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

JG O’GradyAcute liver failurePostgrad Med J811481542005

2 

HC LeeAcute liver failure related to hepatitis B virusHepatol Res38S9S13200810.1111/j.1872-034X.2008.00420.x19125959

3 

Z ZhangZS ZouJL FuSevere dendritic cell perturbation is actively involved in the pathogenesis of acute-on-chronic hepatitis B liver failureJ Hepatol49396406200810.1016/j.jhep.2008.05.01718644645

4 

K RyoY KamogawaI IkedaSignificance of Fas antigen-mediated apoptosis in human fulminant hepatic failureAm J Gastroenterol9520472055200010.1111/j.1572-0241.2000.02268.x10950056

5 

B AlbertsA JohnsonJ LewisMolecular Biology of the Cell4th editionGarland ScienceNew York2002

6 

Y AkazawaGJ GoresDeath receptor-mediated liver injurySemin Liver Dis27327338200710.1055/s-2007-99151017979070

7 

ME GuicciardiGJ GoresApoptosis: a mechanism of acute and chronic liver injuryGut5410241033200510.1136/gut.2004.05385015951554

8 

H MalhiGJ GoresCellular and molecular mechanisms of liver injuryGastroenterology13416411654200810.1053/j.gastro.2008.03.00218471544

9 

SM ElbashirJ HarborthW LendeckelDuplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cellsNature411494498200110.1038/3507810711373684

10 

M YangY LiRW PadgettMicroRNAs: small regulators with a big impactCytokine Growth Factor Rev16387393200510.1016/j.cytogfr.2005.02.00815869899

11 

KD TaganovMP BoldinD BaltimoreMicroRNAs and immunity: tiny player in a big fieldImmunity26133137200710.1016/j.immuni.2007.02.00517307699

12 

S UraM HondaT YamashitaDifferential microRNA expression between hepatitis B and hepatitis C leading disease progression to hepatocellular carcinomaHepatology4910981112200910.1002/hep.2274919173277

13 

O CheungP PuriC EickenNonalcoholic steatohepatitis is associated with altered hepatic microRNA expressionHepatology4818101820200810.1002/hep.2256919030170

14 

RE LanfordES Hildebrandt-EriksenA PetriTherapeutic silencing of microRNA-122 in primates with chronic hepatitis C virus infectionScience327198201201010.1126/science.117817819965718

15 

BK YooPK SanthekadurR GredlerIncreased RNA-induced silencing complex (RISC) activity contributes to hepatocellular carcinomaHepatology5315381548201110.1002/hep.2421621520169

16 

FM AnDS YuQ XieThe role of miRNA-122 expression during the acute liver failure in mice induced by D-GalN/LPSZhonghua Gan Zang Bing Za Zhi185275322010(in Chinese)

17 

FM AnDS YuBD GongThe expression profile and roles of microRNA in tumor necrosis factor-α mediated acute liver failure in mouse modelChin J Infect Dis285495542010(In Chinese)

18 

H MatsumotoS TamuraY KamadaAdiponectin deficiency exacerbates lipopolysaccharide/D-galactosamine-induced liver injury in miceWorld J Gastroenterol1233523358200616733851

19 

BD GongQ XieL WangReal-time quantification of microRNAs in Huh7 cells by stem-loop reverse transcriptase polymerase chain reactionZhonghua Gan Zang Bing Za Zhi176036062009(In Chinese)

20 

KJ LivakTD SchmittgenAnalysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) methodMethods25402408200110.1006/meth.2001.126211846609

21 

MA ReddySD ShuklaPotentiation of mitogen-activated protein kinase by ethanol in embryonic liver cellsBiochem Pharmacol51661668199610.1016/S0006-2952(95)02239-28615903

22 

GQ ZhangH YuXQ ZhouTNF-α induced apoptosis and necrosis of mice hepatocytesWorld Chinese J Digestol83033062000(In Chinese)

23 

E HatanoTumor necrosis factor signaling in hepatocyte apoptosisJ Gastroenterol Hepatol22S43S44200710.1111/j.1440-1746.2006.04645.x17567463

24 

S BalaM MarcosG SzaboEmerging role of microRNAs in liver diseasesWorld J Gastroenterol1556335640200910.3748/wjg.15.563319960558

25 

S YoonTH KimA NatarajanAcute liver injury upregulates microRNA-491-5p in mice, and its overexpression sensitizes Hep G2 cells for tumour necrosis factor-alpha-induced apoptosisLiver Int30376387201010.1111/j.1478-3231.2009.02181.x20015148

26 

AD SharmaN NarainEM HändelMicroRNA-221 Regulates FAS-Induced Fulminant Liver FailureHepatology5316511661201110.1002/hep.2424321400558

27 

B MundtF KühnelL ZenderInvolvement of TRAIL and its receptors in viral hepatitisFASEB J179496200312475902

28 

K StreetzL LeifeldD GrundmannTumor necrosis factor alpha in the pathogenesis of human and murine fulminant hepatic failureGastroenterology119446460200010.1053/gast.2000.936410930380

29 

T Ben MosheH BarashTB KangRole of caspase-8 in hepatocyte response to infection and injury in miceHepatology4510141024200717385212

30 

S LinX LiuR YinInhibitory effects of short hairpin RNA against caspase-8 on apoptosis of murine hepatoma Hepa1–6 cellsBiosci Trends35357200920103947

31 

L ZenderS HutkerC LiedtkeCaspase 8 small interfering RNA prevents acute liver failure in miceProc Natl Acad Sci USA10077977802200310.1073/pnas.133092010012810955

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Yu DS, An FM, Gong BD, Xiang XG, Lin LY, Wang H and Xie Q: The regulatory role of microRNA-1187 in TNF-α-mediated hepatocyte apoptosis in acute liver failure. Int J Mol Med 29: 663-668, 2012.
APA
Yu, D.S., An, F.M., Gong, B.D., Xiang, X.G., Lin, L.Y., Wang, H., & Xie, Q. (2012). The regulatory role of microRNA-1187 in TNF-α-mediated hepatocyte apoptosis in acute liver failure. International Journal of Molecular Medicine, 29, 663-668. https://doi.org/10.3892/ijmm.2012.888
MLA
Yu, D. S., An, F. M., Gong, B. D., Xiang, X. G., Lin, L. Y., Wang, H., Xie, Q."The regulatory role of microRNA-1187 in TNF-α-mediated hepatocyte apoptosis in acute liver failure". International Journal of Molecular Medicine 29.4 (2012): 663-668.
Chicago
Yu, D. S., An, F. M., Gong, B. D., Xiang, X. G., Lin, L. Y., Wang, H., Xie, Q."The regulatory role of microRNA-1187 in TNF-α-mediated hepatocyte apoptosis in acute liver failure". International Journal of Molecular Medicine 29, no. 4 (2012): 663-668. https://doi.org/10.3892/ijmm.2012.888
Copy and paste a formatted citation
x
Spandidos Publications style
Yu DS, An FM, Gong BD, Xiang XG, Lin LY, Wang H and Xie Q: The regulatory role of microRNA-1187 in TNF-α-mediated hepatocyte apoptosis in acute liver failure. Int J Mol Med 29: 663-668, 2012.
APA
Yu, D.S., An, F.M., Gong, B.D., Xiang, X.G., Lin, L.Y., Wang, H., & Xie, Q. (2012). The regulatory role of microRNA-1187 in TNF-α-mediated hepatocyte apoptosis in acute liver failure. International Journal of Molecular Medicine, 29, 663-668. https://doi.org/10.3892/ijmm.2012.888
MLA
Yu, D. S., An, F. M., Gong, B. D., Xiang, X. G., Lin, L. Y., Wang, H., Xie, Q."The regulatory role of microRNA-1187 in TNF-α-mediated hepatocyte apoptosis in acute liver failure". International Journal of Molecular Medicine 29.4 (2012): 663-668.
Chicago
Yu, D. S., An, F. M., Gong, B. D., Xiang, X. G., Lin, L. Y., Wang, H., Xie, Q."The regulatory role of microRNA-1187 in TNF-α-mediated hepatocyte apoptosis in acute liver failure". International Journal of Molecular Medicine 29, no. 4 (2012): 663-668. https://doi.org/10.3892/ijmm.2012.888
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team