Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
April 2012 Volume 29 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
April 2012 Volume 29 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Cloning of the surface layer gene sllB from Bacillus sphaericus ATCC 14577 and its heterologous expression and purification

  • Authors:
    • Yu-Bao Cui
    • Ying Zhou
    • Wei-Na Liu
    • Qing-Wen Chen
    • Gui-Fang Ma
    • Wei-Hong Shi
    • Yun-Gang Wang
    • Li Yang
  • View Affiliations / Copyright

    Affiliations: Department of Laboratory Medicine, Yancheng Health Vocational and Technical College, Yancheng 224006, Jiangsu Province, P.R. China, Huainan Bank of Blood, Huainan 230007, Anhui Province, P.R. China
  • Pages: 677-682
    |
    Published online on: January 19, 2012
       https://doi.org/10.3892/ijmm.2012.890
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

A cDNA fragment encoding the S-layer protein SllB cloned from Bacillus sphaericus ATCC 14577 was expressed on the surface of E. coli BL21 (DE3) cells and confirmed by the square lattice structure at the nanoscale level. The amplified gene fragment designed with PCR primers from a specified reference sequence (GenBank accession no. AJ849550) showed a high degree of sequence identity with the known sequences for S-layer protein. The best alignment scores were seen in B. sphaericus strains JG-A12 and NCTC9602, which code for a pre-form protein with a predicted cleavage site located between the two alanine residues 31 and 32. After this signal peptide sequence was removed, the mature protein had a molecular mass of 116.2613 kDa and a theoretical pI of 5.40. Further bioinformatic analysis revealed three S-layer homology (SLH) domains in the N-terminus of the mature protein, positioned at the 1-61, 63-128 and 137-197 residues. The mature S-layer protein was composed of alpha helices (24.86%), extended strands (27.01%), and rich random coils (48.13%). Bioinformatics-driven characterization of SllB may provide scientific evidence for further application of this gene in the fields of nanobiotechnology and biomimetics in the future.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

MS SidhuL OlsenS-layers of Bacillus speciesMicrobiology14310391052199710.1099/00221287-143-4-1039

2 

H ClausE AkcaT DebaerdemaekerC EvrardJP DeclercqJR HarrisB SchlottH KönigMolecular organization of selected prokaryotic S-layer proteinsCan J Microbiol51731743200510.1139/w05-09316391651

3 

D PumB SchusterM SaraUB SleytrFunctionalisation of surfaces with S-layersIEE Proc Nanobiotechnol1518386200410.1049/ip-nbt:2004063816475848

4 

B SchusterD PumM SáraUB SleytrS-layer protein as key components of a versatile molecular construction kit for biomedical nanotechnologyMini Rev Med Chem6909920200610.2174/13895570677793502616918497

5 

UB SleytrEM EgelseerN IlkD PumB SchusterS-layer as basic building block in a molecular construction kitFEBS J274323334200710.1111/j.1742-4658.2006.05606.x17181542

6 

UB SleytrC HuberN IlkD PumB SchusterEM EgelseerS-layer as a tool kit for nanobiotechnological applicationsFEMS Microbiol Lett267131144200710.1111/j.1574-6968.2006.00573.x17328112

7 

A BreitwieserEM EgelseerD MollN IlkC HotzyB BohleC EbnerUB SletrM SáraA recombinant bacterial cell surface (S-layer)-major birch pollen allergen fusion protein (rSbsC/Bet v1) maintains the ability to self-assemble into regularly structured monomolecular lattices and the functionality of the allergenProtein Eng15243249200210.1093/protein/15.3.243

8 

J LiX HuJ YanZ YuanSpecies-specific cell wall binding affinity of the S-layer proteins of mosquitocidal bacterium Bacillus sphaericus C3-41Appl Environ Microbiol7538913895200910.1128/AEM.00356-0919395560

9 

D MollC HuberB SchlegelD PumUB SleytrM SáraS-layer-streptavidin fusion protein as template for nanopatterned molecular arraysProc Natl Acad Sci USA991464614651200210.1073/pnas.23229939912417763

10 

P MessnerK SteinerK ZarschlerC SchäfferS-layer nanoglycobiology of bacteriaCarbohydr Res34319341951200810.1016/j.carres.2007.12.02518336801

11 

O SkovmandTD OuedraogoE SanogoH SamuelsenLP ToéT BaldetImpact of slow-release Bacillus sphaericus granules on mosquito populations followed in a tropical urban environmentJ Med Entomol4667762009

12 

MA BendaryBacillus thuringiensis and Bacillus sphaericus biopesticides productionJ Basic Microbiol461581702006

13 

PK MittalBiolarvicides in vector control: challenges and prospectsJ Vector Borne Dis402032200315119068

14 

LA LaceyBacillus thuringiensis serovariety israelensis and Bacillus sphaericus for mosquito controlJ Am Mosq Control Assoc23Suppl 2133163200710.2987/8756-971X(2007)23%5B133:BTSIAB%5D2.0.CO;217853604

15 

K PollmannJ RaffM SchnorpfeilG RadevaS Selenska-pobellNovel surface layer protein genes in Bacillus sphaericus associated with unusual insertion elementsMicrobiology15129612973200510.1099/mic.0.28201-016151207

16 

C SchäfferP MessnerThe structure of secondary cell wall polymers: how Gram-positive bacteria stick their cell walls togetherMicrobiology151643651200515758211

17 

S MesnageT FontaineT MignotM DelepierreM MockA FouetBacterial SLH domain proteins are non-covalentaly anchored to the cell surface via a conserved mechanism involving wall polysaccharide pyruvylationEMBO J1944734484200010.1093/emboj/19.17.447310970841

18 

C MaderC HuberD MollUB SleytrM SaraInteraction of the crystalline bacterial cell surface layer protein SbsB and the secondary cell wall polymer of Geobacillus stearothermophilus PV72 assessed by real-time surface plasmon resonance biosensor technologyJ Bacteriol18617581768200410.1128/JB.186.6.1758-1768.200414996807

19 

PM RyzhkovK OstermannG RödelIsolation, gene structure, and comparative analysis of the S-layer gene sslA of Sporosarcina ureae ATCC13881Genetica131255265200710.1007/s10709-006-9135-017242964

20 

YB CuiHX CaiY ZhouCloning, expression and characterization of Der f 7, an allergen of Dermatiophagoides farinae from ChinaJ Med Entomol47868876201010.1093/jmedent/47.5.86820939383

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Cui Y, Zhou Y, Liu W, Chen Q, Ma G, Shi W, Wang Y and Yang L: Cloning of the surface layer gene sllB from Bacillus sphaericus ATCC 14577 and its heterologous expression and purification. Int J Mol Med 29: 677-682, 2012.
APA
Cui, Y., Zhou, Y., Liu, W., Chen, Q., Ma, G., Shi, W. ... Yang, L. (2012). Cloning of the surface layer gene sllB from Bacillus sphaericus ATCC 14577 and its heterologous expression and purification. International Journal of Molecular Medicine, 29, 677-682. https://doi.org/10.3892/ijmm.2012.890
MLA
Cui, Y., Zhou, Y., Liu, W., Chen, Q., Ma, G., Shi, W., Wang, Y., Yang, L."Cloning of the surface layer gene sllB from Bacillus sphaericus ATCC 14577 and its heterologous expression and purification". International Journal of Molecular Medicine 29.4 (2012): 677-682.
Chicago
Cui, Y., Zhou, Y., Liu, W., Chen, Q., Ma, G., Shi, W., Wang, Y., Yang, L."Cloning of the surface layer gene sllB from Bacillus sphaericus ATCC 14577 and its heterologous expression and purification". International Journal of Molecular Medicine 29, no. 4 (2012): 677-682. https://doi.org/10.3892/ijmm.2012.890
Copy and paste a formatted citation
x
Spandidos Publications style
Cui Y, Zhou Y, Liu W, Chen Q, Ma G, Shi W, Wang Y and Yang L: Cloning of the surface layer gene sllB from Bacillus sphaericus ATCC 14577 and its heterologous expression and purification. Int J Mol Med 29: 677-682, 2012.
APA
Cui, Y., Zhou, Y., Liu, W., Chen, Q., Ma, G., Shi, W. ... Yang, L. (2012). Cloning of the surface layer gene sllB from Bacillus sphaericus ATCC 14577 and its heterologous expression and purification. International Journal of Molecular Medicine, 29, 677-682. https://doi.org/10.3892/ijmm.2012.890
MLA
Cui, Y., Zhou, Y., Liu, W., Chen, Q., Ma, G., Shi, W., Wang, Y., Yang, L."Cloning of the surface layer gene sllB from Bacillus sphaericus ATCC 14577 and its heterologous expression and purification". International Journal of Molecular Medicine 29.4 (2012): 677-682.
Chicago
Cui, Y., Zhou, Y., Liu, W., Chen, Q., Ma, G., Shi, W., Wang, Y., Yang, L."Cloning of the surface layer gene sllB from Bacillus sphaericus ATCC 14577 and its heterologous expression and purification". International Journal of Molecular Medicine 29, no. 4 (2012): 677-682. https://doi.org/10.3892/ijmm.2012.890
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team