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International Journal of Molecular Medicine
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April 2012 Volume 29 Issue 4

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Article

ERK and Akt signaling pathways are involved in advanced glycation end product-induced autophagy in rat vascular smooth muscle cells

  • Authors:
    • Pengfei Hu
    • Dongwu Lai
    • Peilin Lu
    • Jing Gao
    • Hong He
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310016, Zhejiang Province, P.R. China, Department of Neurology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310016, Zhejiang Province, P.R. China, Department of Cardiology, The Third People's Hospital of Hangzhou City, Hangzhou 31009, Zhejiang Province, P.R. China
  • Pages: 613-618
    |
    Published online on: January 23, 2012
       https://doi.org/10.3892/ijmm.2012.891
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Abstract

Advanced glycation end products (AGEs) play an important role in the proliferation of vascular smooth muscle cells (VSMCs) and accelerate atherosclerosis in diabetic patients. Autophagy, a life-sustaining process, is stimulated in atherosclerotic plaques by oxidized lipids, inflammation and metabolic stress conditions. In our studies, we utilized MTT assays to show that autophagy is involved in AGE-induced proliferation of VSMCs. Furthermore, treatment with AGEs (100 µg/ml) could induce autophagy in a time- and dose-dependent manner in rat aortic VSMCs. These results were further substantiated by electron microscopy and immunofluorescence imaging. Treatment with AGEs activated ERK, JNK and p38/MAPK, but inhibited Akt. Pretreatment with an ERK inhibitor and an Akt activator inhibited AGE-induced autophagy, demonstrating that AGEs induce autophagy in VSMCs through the ERK and Akt signaling pathways. In addition, RNA interference of RAGE decreased autophagy, indicating that RAGE is pivotal in the process of AGE-induced autophagy. Therefore, AGE-induced autophagy contributes to the process of AGE-induced proliferation of VSMCs, which is related to atherosclerosis in diabetes.
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Copy and paste a formatted citation
Spandidos Publications style
Hu P, Lai D, Lu P, Gao J and He H: ERK and Akt signaling pathways are involved in advanced glycation end product-induced autophagy in rat vascular smooth muscle cells. Int J Mol Med 29: 613-618, 2012.
APA
Hu, P., Lai, D., Lu, P., Gao, J., & He, H. (2012). ERK and Akt signaling pathways are involved in advanced glycation end product-induced autophagy in rat vascular smooth muscle cells. International Journal of Molecular Medicine, 29, 613-618. https://doi.org/10.3892/ijmm.2012.891
MLA
Hu, P., Lai, D., Lu, P., Gao, J., He, H."ERK and Akt signaling pathways are involved in advanced glycation end product-induced autophagy in rat vascular smooth muscle cells". International Journal of Molecular Medicine 29.4 (2012): 613-618.
Chicago
Hu, P., Lai, D., Lu, P., Gao, J., He, H."ERK and Akt signaling pathways are involved in advanced glycation end product-induced autophagy in rat vascular smooth muscle cells". International Journal of Molecular Medicine 29, no. 4 (2012): 613-618. https://doi.org/10.3892/ijmm.2012.891
Copy and paste a formatted citation
x
Spandidos Publications style
Hu P, Lai D, Lu P, Gao J and He H: ERK and Akt signaling pathways are involved in advanced glycation end product-induced autophagy in rat vascular smooth muscle cells. Int J Mol Med 29: 613-618, 2012.
APA
Hu, P., Lai, D., Lu, P., Gao, J., & He, H. (2012). ERK and Akt signaling pathways are involved in advanced glycation end product-induced autophagy in rat vascular smooth muscle cells. International Journal of Molecular Medicine, 29, 613-618. https://doi.org/10.3892/ijmm.2012.891
MLA
Hu, P., Lai, D., Lu, P., Gao, J., He, H."ERK and Akt signaling pathways are involved in advanced glycation end product-induced autophagy in rat vascular smooth muscle cells". International Journal of Molecular Medicine 29.4 (2012): 613-618.
Chicago
Hu, P., Lai, D., Lu, P., Gao, J., He, H."ERK and Akt signaling pathways are involved in advanced glycation end product-induced autophagy in rat vascular smooth muscle cells". International Journal of Molecular Medicine 29, no. 4 (2012): 613-618. https://doi.org/10.3892/ijmm.2012.891
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