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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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May 2012 Volume 29 Issue 5

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Medicine International

An International Open Access Journal Devoted to General Medicine.

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May 2012 Volume 29 Issue 5

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Article

Molecular profile of organ culture-stored corneal epithelium: Lgr5 is a potential new phenotypic marker of residual human corneal limbal epithelial stem cells

  • Authors:
    • Joanna Brzeszczynska
    • Kanna Ramaesh
    • Bal Dhillon
    • James A. Ross

  • View Affiliations / Copyright

    Affiliations: Tissue Injury and Repair Group, University of Edinburgh - MRC Centre for Regenerative Medicine, Edinburgh, Scotland, UK, Tennent Institute of Ophthalmology, Gartnavel General Hospital, Glasgow, Scotland, UK, Department of Clinical and Surgical Sciences, Ophthalmology Section, Princess Alexandra Eye Pavilion, Royal Infirmary of Edinburgh, Edinburgh, Scotland, UK
  • Pages: 871-876
    |
    Published online on: February 6, 2012
       https://doi.org/10.3892/ijmm.2012.904
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Abstract

Long-term preservation of corneal limbal epithelium may decrease its quality and change the molecular signature of the limbal epithelial stem cells. In this study we have investi­gated the molecular profile of isolated corneal epithelial cells that have been in storage for an extended time. Isolated cells were characterised by the expression profile of different cytokeratins and markers of squamous metaplasia (vimentin and α‑actin). Furthermore, we examined global markers of adult stem cells including p63α and ABCG2 but also Lgr5 as a novel stem cell marker. Immunocytochemical staining and PCR analysis of p63α, ABCG2 and Lgr5 revealed the existence of side-population cells with a stem-cell phenotype and maintenance of corneal limbal stem cell properties. Lgr5 expression can be related to cellular stemness and can be considered as a new phenotypic marker of residual human corneal limbal stem cells. However, the existence of ck10 together with co-expressed α-actin and vimentin suggests that the corneas investigated were under oxidative stress and showed evidence of squamous metaplasia.

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Copy and paste a formatted citation
Spandidos Publications style
Brzeszczynska J, Ramaesh K, Dhillon B and Ross JA: Molecular profile of organ culture-stored corneal epithelium: Lgr5 is a potential new phenotypic marker of residual human corneal limbal epithelial stem cells. Int J Mol Med 29: 871-876, 2012.
APA
Brzeszczynska, J., Ramaesh, K., Dhillon, B., & Ross, J.A. (2012). Molecular profile of organ culture-stored corneal epithelium: Lgr5 is a potential new phenotypic marker of residual human corneal limbal epithelial stem cells. International Journal of Molecular Medicine, 29, 871-876. https://doi.org/10.3892/ijmm.2012.904
MLA
Brzeszczynska, J., Ramaesh, K., Dhillon, B., Ross, J. A."Molecular profile of organ culture-stored corneal epithelium: Lgr5 is a potential new phenotypic marker of residual human corneal limbal epithelial stem cells". International Journal of Molecular Medicine 29.5 (2012): 871-876.
Chicago
Brzeszczynska, J., Ramaesh, K., Dhillon, B., Ross, J. A."Molecular profile of organ culture-stored corneal epithelium: Lgr5 is a potential new phenotypic marker of residual human corneal limbal epithelial stem cells". International Journal of Molecular Medicine 29, no. 5 (2012): 871-876. https://doi.org/10.3892/ijmm.2012.904
Copy and paste a formatted citation
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Spandidos Publications style
Brzeszczynska J, Ramaesh K, Dhillon B and Ross JA: Molecular profile of organ culture-stored corneal epithelium: Lgr5 is a potential new phenotypic marker of residual human corneal limbal epithelial stem cells. Int J Mol Med 29: 871-876, 2012.
APA
Brzeszczynska, J., Ramaesh, K., Dhillon, B., & Ross, J.A. (2012). Molecular profile of organ culture-stored corneal epithelium: Lgr5 is a potential new phenotypic marker of residual human corneal limbal epithelial stem cells. International Journal of Molecular Medicine, 29, 871-876. https://doi.org/10.3892/ijmm.2012.904
MLA
Brzeszczynska, J., Ramaesh, K., Dhillon, B., Ross, J. A."Molecular profile of organ culture-stored corneal epithelium: Lgr5 is a potential new phenotypic marker of residual human corneal limbal epithelial stem cells". International Journal of Molecular Medicine 29.5 (2012): 871-876.
Chicago
Brzeszczynska, J., Ramaesh, K., Dhillon, B., Ross, J. A."Molecular profile of organ culture-stored corneal epithelium: Lgr5 is a potential new phenotypic marker of residual human corneal limbal epithelial stem cells". International Journal of Molecular Medicine 29, no. 5 (2012): 871-876. https://doi.org/10.3892/ijmm.2012.904
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