Adenovirally delivered IFN-β exerts antitumor effects through transient T-lymphocyte depletion and Ag-specific T-cell proliferation

  • Authors:
    • Sang-Seok Oh
    • Chaerin Moon
    • Dong-Hyeok Kim
    • Hyunkeun  Song
    • Saegwang  Park
    • Yangxin Fu
    • Kwang Dong Kim
  • View Affiliations

  • Published online on: March 13, 2012     https://doi.org/10.3892/ijmm.2012.936
  • Pages: 1153-1157
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Abstract

Type I interferons (IFNs), including IFN-β, are known to enhance antigen (Ag) presentation and to promote the expansion, survival and effector function of CD8+ cytotoxic lymphocytes (CTL) during viral infections. Furthermore, IFN-β is a potent candidate for antitumor drugs; however, recombinant IFN-β is too unstable for use in tumor therapy in vivo. In this study, we therefore examined the efficacy and mechanism of exogenous IFN-β as a biomolecule for tumor therapy, using adenovirus encoding IFN-β (Ad-IFNβ) as a therapeutic agent in a mouse model. Ag104Ld and 4T1 tumor cells exposed to Ad-IFNβ showed growth retardation and cell death in vitro, and tumor growth as well as tumor metastasis was inhibited in vivo. The Ad-IFNβ-mediated antitumor effect was dependent on CD8+ T cells in vivo, rather than on a direct cytotoxic effect of Ad-IFNβ. Transient T lymphocyte depletion was observed in tumor tissue after intratumoral injection with Ad-IFNβ. Despite the T lymphocyte depletion, the proliferation of Ag-specific CD8+ T cells was increased in Ad-IFNβ-treated mice compared to control virus-treated mice. These results suggest that IFN-β might contribute to the inhibition of tumor growth by depleting Ag-nonspecific T lymphocytes and enhancing proliferation of Ag-specific CD8+ T cells.

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June 2012
Volume 29 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Oh S, Moon C, Kim D, Song H, Park S, Fu Y and Kim KD: Adenovirally delivered IFN-β exerts antitumor effects through transient T-lymphocyte depletion and Ag-specific T-cell proliferation. Int J Mol Med 29: 1153-1157, 2012
APA
Oh, S., Moon, C., Kim, D., Song, H., Park, S., Fu, Y., & Kim, K.D. (2012). Adenovirally delivered IFN-β exerts antitumor effects through transient T-lymphocyte depletion and Ag-specific T-cell proliferation. International Journal of Molecular Medicine, 29, 1153-1157. https://doi.org/10.3892/ijmm.2012.936
MLA
Oh, S., Moon, C., Kim, D., Song, H., Park, S., Fu, Y., Kim, K. D."Adenovirally delivered IFN-β exerts antitumor effects through transient T-lymphocyte depletion and Ag-specific T-cell proliferation". International Journal of Molecular Medicine 29.6 (2012): 1153-1157.
Chicago
Oh, S., Moon, C., Kim, D., Song, H., Park, S., Fu, Y., Kim, K. D."Adenovirally delivered IFN-β exerts antitumor effects through transient T-lymphocyte depletion and Ag-specific T-cell proliferation". International Journal of Molecular Medicine 29, no. 6 (2012): 1153-1157. https://doi.org/10.3892/ijmm.2012.936