Proteomic analysis of oxidative modification in endothelial colony-forming cells treated by hydrogen peroxide

  • Authors:
    • Jun Wei
    • Ying Liu
    • Ming Chang
    • Chong-Ling Sun
    • Da-Wei Li
    • Zhi-Qiang Liu
    • Lin-Sen Hu
  • View Affiliations

  • Published online on: March 19, 2012     https://doi.org/10.3892/ijmm.2012.944
  • Pages: 1099-1105
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Abstract

Endothelial progenitor cells (EPCs) which circulate in the peripheral blood and reside in blood vessels are proven to promote the repair of damaged endothelium and improve the function of endothelial cells after vascular injury. Recently, EPCs have been extensively studied as risk biomarkers and a potential therapeutic tool for cardiovascular disease. It is known that oxidative stress is one of the most important pathogenetic factors impairing endothelial function. During the repair process after endothelial injury, EPCs are exposed to oxidative stress. In this study, we treated endothelial colony-forming cells (ECFCs) with hydrogen peroxide (H2O2) as an oxidative stress model and observed the changes in cytology and morphology of ECFCs. In addition, we investigated the alterations in oxidative levels of proteins associated with H2O2-induced morphological and cytological changes in ECFCs by proteomic analysis of oxidative modification. The results showed that H2O2 treatment led to a decreased proliferation, increased apoptosis and impaired tube-forming ability of ECFCs in a dose-dependent manner. Five proteins with upregulated oxidative levels were identified successfully. The upregulated oxidative levels of these five proteins may be responsible for the dysfunction of ECFCs under oxidative stress. Our results may provide some novel insights into the molecular mechanisms of oxidative stress action on ECFCs.

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June 2012
Volume 29 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Copy and paste a formatted citation
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Spandidos Publications style
Wei J, Liu Y, Chang M, Sun C, Li D, Liu Z and Hu L: Proteomic analysis of oxidative modification in endothelial colony-forming cells treated by hydrogen peroxide. Int J Mol Med 29: 1099-1105, 2012
APA
Wei, J., Liu, Y., Chang, M., Sun, C., Li, D., Liu, Z., & Hu, L. (2012). Proteomic analysis of oxidative modification in endothelial colony-forming cells treated by hydrogen peroxide. International Journal of Molecular Medicine, 29, 1099-1105. https://doi.org/10.3892/ijmm.2012.944
MLA
Wei, J., Liu, Y., Chang, M., Sun, C., Li, D., Liu, Z., Hu, L."Proteomic analysis of oxidative modification in endothelial colony-forming cells treated by hydrogen peroxide". International Journal of Molecular Medicine 29.6 (2012): 1099-1105.
Chicago
Wei, J., Liu, Y., Chang, M., Sun, C., Li, D., Liu, Z., Hu, L."Proteomic analysis of oxidative modification in endothelial colony-forming cells treated by hydrogen peroxide". International Journal of Molecular Medicine 29, no. 6 (2012): 1099-1105. https://doi.org/10.3892/ijmm.2012.944