Apple polysaccharides induce apoptosis in colorectal cancer cells

Zhang,Dian; Sun,Yang; Yue,Zhenggang; Li,Qian; Meng,Jin; Liu,Junajuan; Hekong,Xiang; Jiang,Fengliang; Mi,Man; Liu,Li; Mei,Qibing

doi:10.3892/ijmm.2012.953

July 2012 Volume 30 Issue 1

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July 2012 Volume 30 Issue 1

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Article

Apple polysaccharides induce apoptosis in colorectal cancer cells

Authors:
- Dian Zhang
- Yang Sun
- Zhenggang Yue
- Qian Li
- Jin Meng
- Junajuan Liu
- Xiang Hekong
- Fengliang Jiang
- Man Mi
- Li Liu
- Qibing Mei
View Affiliations / Copyright

Affiliations: Department of Pathogen Biology and Immunology, Xi'an Medical College, Xi'an 710021, Shaanxi, P.R. China, Key Laboratory of Gastrointestinal Pharmacology of Chinese Materia Medica of the State Administration of Traditional Chinese Medicine, Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, P.R. China, Faculty of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, P.R. China
Pages: 100-106
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Published online on: March 26, 2012

https://doi.org/10.3892/ijmm.2012.953
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Abstract

Certain components of apples have been shown to prevent cancer growth and impede cancer progression. We hypothesized that extracted apple polysaccharides (APs) might, therefore, have anticancer effects, through a mechanism involving the induction of apoptosis in cancer cells, partly via the NF-κB pathway. Two human colorectal cancer (CRC) cell lines, HT-29 and SW620, were exposed to different concentrations of APs (0.01, 0.1 or 1 mg/ml). Cell apoptosis was measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay by flow cytometry and incorporation of 5'-bromodeoxyuridine (BrdU) into DNA to identify the proliferating cell fraction, using fluorescence microscopy in vitro. The protein levels of NF-κB/p65, I-κBα, pI-κBα, Bax, Bcl-xl and Bcl-2 were evaluated by western blotting. The target sites of APs on CRC cells were assessed by flow cytometry. At concentrations of 0.1 and 1 mg/ml, APs showed apoptosis-inducing effects, increased expressions of Bax, nuclear p65 and cytoplasmic pI-κBα, and decreased expressions of Bcl-2, Bcl-xl and cytoplasmic I-κBα. APs induced apoptosis by slightly activating the NF-κB pathway; the AP target site could be the Toll-like receptor 4 on the cell membrane. These results demonstrate the potential of APs as agents for clinical prevention and treatment of CRC.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Apple polysaccharides induce apoptosis in colorectal cancer cells

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Abstract

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