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Article

DMP1 is a target of let-7 in dental pulp cells

  • Authors:
    • Jing Yue
    • Buling Wu
    • Jie Gao
    • Xin Huang
    • Changxia Li
    • Dandan Ma
    • Fuchun Fang
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, Nanfang Hospital, College of Stomatology, Southern Medical University, Guangzhou, P.R. China
  • Pages: 295-301
    |
    Published online on: April 25, 2012
       https://doi.org/10.3892/ijmm.2012.982
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Abstract

Members of the let-7 family have been shown to play a critical role in cell differentiation and tumorigenesis. However, potential targets of let-7 are still unclear. In the current study, we used bioinformatic analysis combined with DNA sequence analysis to identify potential let-7 targets. We discovered that dentin matrix protein 1 (DMP1), which is a non-collagenous protein essential in the mineralization of dentin and bone, has a let-7 binding site in its 3'-untranslated region. Furthermore, reporter assays demonstrated that the DMP1 3'-untranslated region can be regulated directly by the members of let-7. Gene expression levels of let-7 and DMP1 were validated by qRT-PCR of dental pulp cells cultured in a mineralizing medium. Our results suggest that DMP1 is regulated post-transcriptionally by let-7 during odontoblast differentiation.
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Copy and paste a formatted citation
Spandidos Publications style
Yue J, Wu B, Gao J, Huang X, Li C, Ma D and Fang F: DMP1 is a target of let-7 in dental pulp cells. Int J Mol Med 30: 295-301, 2012.
APA
Yue, J., Wu, B., Gao, J., Huang, X., Li, C., Ma, D., & Fang, F. (2012). DMP1 is a target of let-7 in dental pulp cells. International Journal of Molecular Medicine, 30, 295-301. https://doi.org/10.3892/ijmm.2012.982
MLA
Yue, J., Wu, B., Gao, J., Huang, X., Li, C., Ma, D., Fang, F."DMP1 is a target of let-7 in dental pulp cells". International Journal of Molecular Medicine 30.2 (2012): 295-301.
Chicago
Yue, J., Wu, B., Gao, J., Huang, X., Li, C., Ma, D., Fang, F."DMP1 is a target of let-7 in dental pulp cells". International Journal of Molecular Medicine 30, no. 2 (2012): 295-301. https://doi.org/10.3892/ijmm.2012.982
Copy and paste a formatted citation
x
Spandidos Publications style
Yue J, Wu B, Gao J, Huang X, Li C, Ma D and Fang F: DMP1 is a target of let-7 in dental pulp cells. Int J Mol Med 30: 295-301, 2012.
APA
Yue, J., Wu, B., Gao, J., Huang, X., Li, C., Ma, D., & Fang, F. (2012). DMP1 is a target of let-7 in dental pulp cells. International Journal of Molecular Medicine, 30, 295-301. https://doi.org/10.3892/ijmm.2012.982
MLA
Yue, J., Wu, B., Gao, J., Huang, X., Li, C., Ma, D., Fang, F."DMP1 is a target of let-7 in dental pulp cells". International Journal of Molecular Medicine 30.2 (2012): 295-301.
Chicago
Yue, J., Wu, B., Gao, J., Huang, X., Li, C., Ma, D., Fang, F."DMP1 is a target of let-7 in dental pulp cells". International Journal of Molecular Medicine 30, no. 2 (2012): 295-301. https://doi.org/10.3892/ijmm.2012.982
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