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International Journal of Molecular Medicine
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Article

Proteomic analysis of calcified abdominal and thoracic aortic aneurysms

  • Authors:
    • Ken-Ichi Matsumoto
    • Tomoko Maniwa
    • Tetsuya Tanaka
    • Kazumi Satoh
    • Hideki Okunishi
    • Teiji Oda
  • View Affiliations / Copyright

    Affiliations: Department of Biosignaling and Radioisotope Experiment, Center for Integrated Research in Science, Shimane University, Izumo, Japan, Department of Pharmacology, Shimane University School of Medicine, Izumo, Japan, Division of Cardiovascular and Thoracic Surgery, Department of Surgery, Shimane University School of Medicine, Izumo, Japan
  • Pages: 417-429
    |
    Published online on: April 30, 2012
       https://doi.org/10.3892/ijmm.2012.985
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Abstract

Aortic aneurysm is a complex multifactorial disease with genetic and environmental risk factors. It is often accompanied by aortic calcification. Here, to uncover proteins that are significantly changed in calcified abdominal aortic aneurysms (CAAs) and calcified thoracic aortic aneurysms (CTAs) compared with those in adjacent normal aorta tissues, comprehensive analysis of differentially expressed proteins in their tissues was performed by a quantitative proteomic approach with iTRAQ labeling in combination with nanoLC-MALDI-TOF/TOF-MS/MS followed by ProteinPilot analysis. The proteomic analysis revealed 138 and 134 proteins differentially expressed in CAAs and CTAs in contrast to neighboring normal aorta tissues with high confidence, respectively. Significantly increased expression (≥1.3-fold) was found in 41 and 28 proteins, whereas decreased expression (<0.77-fold) was found in 4 and 60 proteins in CAAs and CTAs, respectively. Among them, we identified already known proteins involved in aneurysm formation and vascular calcification, such as type I and III collagen, matrix Gla protein, and α-2-HS-glycoprotein in CAAs and fibrinogen α, β and γ chains and α-2-HS-glycoprotein in CTAs with increased expression and mimecan in CAAs and fibulin-5 in CTAs with decreased expression. Based on the Panther pathway and Genesis clustering analyses, some of the proteins could be linked to corresponding biochemical pathways, such as the integrin signaling pathway with increased expression in CAAs, the blood coagulation pathway with increased expression in CTAs, and the inflammation mediated by chemokine and cytokine signaling pathway and the glycolysis pathway with decreased expression in CTAs. Interestingly, it was found by clustering analysis that samples from CAAs of patients with both CAAs and CTAs were clustered outside the samples of patients with CAAs and were clustered with samples of patients with CTAs. Our results provide a comprehensive patient-based proteomic analysis for the identification of potential biomarkers for CAAs and CTAs.
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Copy and paste a formatted citation
Spandidos Publications style
Matsumoto K, Maniwa T, Tanaka T, Satoh K, Okunishi H and Oda T: Proteomic analysis of calcified abdominal and thoracic aortic aneurysms. Int J Mol Med 30: 417-429, 2012.
APA
Matsumoto, K., Maniwa, T., Tanaka, T., Satoh, K., Okunishi, H., & Oda, T. (2012). Proteomic analysis of calcified abdominal and thoracic aortic aneurysms. International Journal of Molecular Medicine, 30, 417-429. https://doi.org/10.3892/ijmm.2012.985
MLA
Matsumoto, K., Maniwa, T., Tanaka, T., Satoh, K., Okunishi, H., Oda, T."Proteomic analysis of calcified abdominal and thoracic aortic aneurysms". International Journal of Molecular Medicine 30.2 (2012): 417-429.
Chicago
Matsumoto, K., Maniwa, T., Tanaka, T., Satoh, K., Okunishi, H., Oda, T."Proteomic analysis of calcified abdominal and thoracic aortic aneurysms". International Journal of Molecular Medicine 30, no. 2 (2012): 417-429. https://doi.org/10.3892/ijmm.2012.985
Copy and paste a formatted citation
x
Spandidos Publications style
Matsumoto K, Maniwa T, Tanaka T, Satoh K, Okunishi H and Oda T: Proteomic analysis of calcified abdominal and thoracic aortic aneurysms. Int J Mol Med 30: 417-429, 2012.
APA
Matsumoto, K., Maniwa, T., Tanaka, T., Satoh, K., Okunishi, H., & Oda, T. (2012). Proteomic analysis of calcified abdominal and thoracic aortic aneurysms. International Journal of Molecular Medicine, 30, 417-429. https://doi.org/10.3892/ijmm.2012.985
MLA
Matsumoto, K., Maniwa, T., Tanaka, T., Satoh, K., Okunishi, H., Oda, T."Proteomic analysis of calcified abdominal and thoracic aortic aneurysms". International Journal of Molecular Medicine 30.2 (2012): 417-429.
Chicago
Matsumoto, K., Maniwa, T., Tanaka, T., Satoh, K., Okunishi, H., Oda, T."Proteomic analysis of calcified abdominal and thoracic aortic aneurysms". International Journal of Molecular Medicine 30, no. 2 (2012): 417-429. https://doi.org/10.3892/ijmm.2012.985
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