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Article

Picroside II protects cardiomyocytes from hypoxia/reoxygenation-induced apoptosis by activating the PI3K/Akt and CREB pathways

  • Authors:
    • Fan-Ji Meng
    • Su-Min Jiao
    • Bo Yu
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, The First Affiliated Hospital of China Medical University, Shenyang, P.R. China
  • Pages: 263-270
    |
    Published online on: May 9, 2012
       https://doi.org/10.3892/ijmm.2012.987
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Abstract

Picroside II, an iridoid glucoside found in the root of Picrorhiza scrophulariiflora Pennell (Scrophulariaceae), has been demonstrated to reduce apoptosis in neuronal cells and other cell types. However, whether picroside II has a protective effect against cardiomyocyte apoptosis is poorly understood. In the present study, we investigated the cardioprotective role of picroside II and the underlying mechanisms in hypoxia/reoxygenation-induced cardiomyocyte apoptosis. The pretreatment with picroside II markedly attenuated hypoxia/reoxygenation-induced cell damage dose-dependently, which was evident by the increased cell viability and the corresponding decrease in lactate dehydrogenase release (LDH). The pretreatment with picroside II inhibited apoptosis confirmed by Annexin V-FITC staining, Hoechst 33258 nuclear staining and by assessment of caspase-3 activity. In addition, we found that picroside II not only increased the expression of Bcl-2, while decreasing Bax expression, but also augmented Akt and cAMP response element-binding protein (CREB) phosphorylation and ultimately inhibited hypoxia/reoxygenation-induced apoptosis. Furthermore, the protective effects of picroside II were abrogated by pretreatment of the cells with wortmannin or LY294002, a specific PI3K inhibitor. The present study suggests that picroside II inhibits hypoxia/reoxygenation-induced apoptosis in cardiomyocytes by activating the PI3K/Akt and CREB pathways and modulating expression of Bcl-2 and Bax.
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Copy and paste a formatted citation
Spandidos Publications style
Meng F, Jiao S and Yu B: Picroside II protects cardiomyocytes from hypoxia/reoxygenation-induced apoptosis by activating the PI3K/Akt and CREB pathways. Int J Mol Med 30: 263-270, 2012.
APA
Meng, F., Jiao, S., & Yu, B. (2012). Picroside II protects cardiomyocytes from hypoxia/reoxygenation-induced apoptosis by activating the PI3K/Akt and CREB pathways. International Journal of Molecular Medicine, 30, 263-270. https://doi.org/10.3892/ijmm.2012.987
MLA
Meng, F., Jiao, S., Yu, B."Picroside II protects cardiomyocytes from hypoxia/reoxygenation-induced apoptosis by activating the PI3K/Akt and CREB pathways". International Journal of Molecular Medicine 30.2 (2012): 263-270.
Chicago
Meng, F., Jiao, S., Yu, B."Picroside II protects cardiomyocytes from hypoxia/reoxygenation-induced apoptosis by activating the PI3K/Akt and CREB pathways". International Journal of Molecular Medicine 30, no. 2 (2012): 263-270. https://doi.org/10.3892/ijmm.2012.987
Copy and paste a formatted citation
x
Spandidos Publications style
Meng F, Jiao S and Yu B: Picroside II protects cardiomyocytes from hypoxia/reoxygenation-induced apoptosis by activating the PI3K/Akt and CREB pathways. Int J Mol Med 30: 263-270, 2012.
APA
Meng, F., Jiao, S., & Yu, B. (2012). Picroside II protects cardiomyocytes from hypoxia/reoxygenation-induced apoptosis by activating the PI3K/Akt and CREB pathways. International Journal of Molecular Medicine, 30, 263-270. https://doi.org/10.3892/ijmm.2012.987
MLA
Meng, F., Jiao, S., Yu, B."Picroside II protects cardiomyocytes from hypoxia/reoxygenation-induced apoptosis by activating the PI3K/Akt and CREB pathways". International Journal of Molecular Medicine 30.2 (2012): 263-270.
Chicago
Meng, F., Jiao, S., Yu, B."Picroside II protects cardiomyocytes from hypoxia/reoxygenation-induced apoptosis by activating the PI3K/Akt and CREB pathways". International Journal of Molecular Medicine 30, no. 2 (2012): 263-270. https://doi.org/10.3892/ijmm.2012.987
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