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Article

Genistein inhibits MDA-MB-231 triple-negative breast cancer cell growth by inhibiting NF-κB activity via the Notch-1 pathway

  • Authors:
    • Hong Pan
    • Wenbin Zhou
    • Wei He
    • Xiaoan Liu
    • Qiang Ding
    • Lijun Ling
    • Xiaoming Zha
    • Shui Wang
  • View Affiliations / Copyright

    Affiliations: Department of Breast Surgery, The First Affiliated Hospital of Nanjing Medical University, 210029 Nanjing, P.R. China, Department of Endocrinology and Metabolism, The First Affiliated Hospital of Nanjing Medical University, 210029 Nanjing, P.R. China
  • Pages: 337-343
    |
    Published online on: May 9, 2012
       https://doi.org/10.3892/ijmm.2012.990
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Abstract

Genistein (Gen) has been reported as a protective factor against breast cancer. However, the molecular mechanism by which Gen elicits its effects on triple-negative breast cancer cells has not been fully elucidated. In our study, the breast cancer cell line MDA-MB-231 was selected to determine the action of Gen on triple-negative breast cancer cells. MTT assay, flow cytometric analysis, siRNA transfection, western blotting and nuclear factor-κB (NF-κB) activation-nuclear translocation assay were used to address the role of NF-κB activity and the Notch-1 signaling pathway on the effects of Gen. Our study revealed that Gen elicited a dramatic effect on cell growth inhibition, in a dose-dependent and time-dependent manner. Treatment of MDA-MB-231 cells with 0, 5, 10 or 20 µM Gen induced apoptosis of 6.78, 18.98, 30.45 and 60.64%, respectively. Exposure of MDA-MB-231 cells to Gen also resulted in G2/M phase accumulation of cells corresponding to 4.93, 12.54, 18.93 and 30.95%, respectively. Furthermore, our data demonstrated for the first time that Gen inhibited the growth of MDA-MB-231 triple-negative breast cancer cells by inhibiting NF-κB activity via the Nocth-1 signaling pathway in a dose-dependent manner. We also found that Gen downregulated the expression of cyclin B1, Bcl-2 and Bcl-xL, possibly mediated by NF-κB activation via the Notch-1 signaling pathway. In conclusion, our results suggest that inhibition of NF-κB activity via the Notch-1 pathway may be a novel mechanism by which Gen suppresses the growth of triple-negative breast cancer cells. Further preclinical and clinical studies are warranted to further investigate the application of Gen for the treatment of triple-negative breast cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Pan H, Zhou W, He W, Liu X, Ding Q, Ling L, Zha X and Wang S: Genistein inhibits MDA-MB-231 triple-negative breast cancer cell growth by inhibiting NF-κB activity via the Notch-1 pathway. Int J Mol Med 30: 337-343, 2012.
APA
Pan, H., Zhou, W., He, W., Liu, X., Ding, Q., Ling, L. ... Wang, S. (2012). Genistein inhibits MDA-MB-231 triple-negative breast cancer cell growth by inhibiting NF-κB activity via the Notch-1 pathway. International Journal of Molecular Medicine, 30, 337-343. https://doi.org/10.3892/ijmm.2012.990
MLA
Pan, H., Zhou, W., He, W., Liu, X., Ding, Q., Ling, L., Zha, X., Wang, S."Genistein inhibits MDA-MB-231 triple-negative breast cancer cell growth by inhibiting NF-κB activity via the Notch-1 pathway". International Journal of Molecular Medicine 30.2 (2012): 337-343.
Chicago
Pan, H., Zhou, W., He, W., Liu, X., Ding, Q., Ling, L., Zha, X., Wang, S."Genistein inhibits MDA-MB-231 triple-negative breast cancer cell growth by inhibiting NF-κB activity via the Notch-1 pathway". International Journal of Molecular Medicine 30, no. 2 (2012): 337-343. https://doi.org/10.3892/ijmm.2012.990
Copy and paste a formatted citation
x
Spandidos Publications style
Pan H, Zhou W, He W, Liu X, Ding Q, Ling L, Zha X and Wang S: Genistein inhibits MDA-MB-231 triple-negative breast cancer cell growth by inhibiting NF-κB activity via the Notch-1 pathway. Int J Mol Med 30: 337-343, 2012.
APA
Pan, H., Zhou, W., He, W., Liu, X., Ding, Q., Ling, L. ... Wang, S. (2012). Genistein inhibits MDA-MB-231 triple-negative breast cancer cell growth by inhibiting NF-κB activity via the Notch-1 pathway. International Journal of Molecular Medicine, 30, 337-343. https://doi.org/10.3892/ijmm.2012.990
MLA
Pan, H., Zhou, W., He, W., Liu, X., Ding, Q., Ling, L., Zha, X., Wang, S."Genistein inhibits MDA-MB-231 triple-negative breast cancer cell growth by inhibiting NF-κB activity via the Notch-1 pathway". International Journal of Molecular Medicine 30.2 (2012): 337-343.
Chicago
Pan, H., Zhou, W., He, W., Liu, X., Ding, Q., Ling, L., Zha, X., Wang, S."Genistein inhibits MDA-MB-231 triple-negative breast cancer cell growth by inhibiting NF-κB activity via the Notch-1 pathway". International Journal of Molecular Medicine 30, no. 2 (2012): 337-343. https://doi.org/10.3892/ijmm.2012.990
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