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August 2012 Volume 30 Issue 2

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Article

The effects of siRNA against RPL22 on ET-1-induced proliferation of human pulmonary arterial smooth muscle cells

  • Authors:
    • Kai Sun
    • Hong Xue
    • Hui Wang
    • Qiang Wang
    • Xiang-Rong Zuo
    • Wei-Ping Xie
    • Hong Wang
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, P.R. China
  • Pages: 351-357
    |
    Published online on: May 9, 2012
       https://doi.org/10.3892/ijmm.2012.992
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Abstract

The aim of this study was to investigate the effects of small interference RNA (siRNA) against ribosomal protein L22 (RPL22) on ET-1-induced proliferation of human pulmonary arterial smooth muscle cells (HPASMCs). HPASMCs were transfected with siRNA against RPL22, incubated in smooth muscle cell culture medium (SMCM) and ET-1. RPL22 gene expression and protein levels of HPASMCs were measured by real-time PCR and western blotting, respectively. PCNA, CCK-8 immunohistochemistry and flow cytometry analysis were used to evaluate HPASMC proliferation. Cyclin D1 (CCND1) expression was also assayed. Following transfection with siRNA against RPL22, RPL22 expression was significantly inhibited in the control and ET-1 groups. HPASMC proliferation was significantly suppressed by transfection with siRNA against RPL22. Moreover, CCND1 was also downregulated by inhibiting RPL22 expression. In conclusion, these data suggest that inhibition of RPL22 expression can suppress HPASMC proliferation and CCND1 expression. The effects of siRNA against RPL22 on HPASMC proliferation are considered to be mediated through inhibition of CCND1 expression.
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Copy and paste a formatted citation
Spandidos Publications style
Sun K, Xue H, Wang H, Wang Q, Zuo X, Xie W and Wang H: The effects of siRNA against RPL22 on ET-1-induced proliferation of human pulmonary arterial smooth muscle cells. Int J Mol Med 30: 351-357, 2012.
APA
Sun, K., Xue, H., Wang, H., Wang, Q., Zuo, X., Xie, W., & Wang, H. (2012). The effects of siRNA against RPL22 on ET-1-induced proliferation of human pulmonary arterial smooth muscle cells. International Journal of Molecular Medicine, 30, 351-357. https://doi.org/10.3892/ijmm.2012.992
MLA
Sun, K., Xue, H., Wang, H., Wang, Q., Zuo, X., Xie, W., Wang, H."The effects of siRNA against RPL22 on ET-1-induced proliferation of human pulmonary arterial smooth muscle cells". International Journal of Molecular Medicine 30.2 (2012): 351-357.
Chicago
Sun, K., Xue, H., Wang, H., Wang, Q., Zuo, X., Xie, W., Wang, H."The effects of siRNA against RPL22 on ET-1-induced proliferation of human pulmonary arterial smooth muscle cells". International Journal of Molecular Medicine 30, no. 2 (2012): 351-357. https://doi.org/10.3892/ijmm.2012.992
Copy and paste a formatted citation
x
Spandidos Publications style
Sun K, Xue H, Wang H, Wang Q, Zuo X, Xie W and Wang H: The effects of siRNA against RPL22 on ET-1-induced proliferation of human pulmonary arterial smooth muscle cells. Int J Mol Med 30: 351-357, 2012.
APA
Sun, K., Xue, H., Wang, H., Wang, Q., Zuo, X., Xie, W., & Wang, H. (2012). The effects of siRNA against RPL22 on ET-1-induced proliferation of human pulmonary arterial smooth muscle cells. International Journal of Molecular Medicine, 30, 351-357. https://doi.org/10.3892/ijmm.2012.992
MLA
Sun, K., Xue, H., Wang, H., Wang, Q., Zuo, X., Xie, W., Wang, H."The effects of siRNA against RPL22 on ET-1-induced proliferation of human pulmonary arterial smooth muscle cells". International Journal of Molecular Medicine 30.2 (2012): 351-357.
Chicago
Sun, K., Xue, H., Wang, H., Wang, Q., Zuo, X., Xie, W., Wang, H."The effects of siRNA against RPL22 on ET-1-induced proliferation of human pulmonary arterial smooth muscle cells". International Journal of Molecular Medicine 30, no. 2 (2012): 351-357. https://doi.org/10.3892/ijmm.2012.992
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