Global transcriptional profiling of the postmortem brain of a patient with G114V genetic Creutzfeldt-Jakob disease

  • Authors:
    • Chan Tian
    • Di Liu
    • Chen Chen
    • Yin Xu
    • Han-Shi Gong
    • Cao Chen
    • Qi Shi
    • Bao-Yun Zhang
    • Jun Han
    • Xiao-Ping Dong
  • View Affiliations

  • Published online on: January 10, 2013     https://doi.org/10.3892/ijmm.2013.1239
  • Pages: 676-688
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Abstract

Familial or genetic Creutzfeldt-Jakob disease (fCJD or gCJD) is an inherent human prion disease caused by mutation of the prion protein gene (PRNP). In the present study, global expression patterns of the parietal cortex from a patient with G114V gCJD were analyzed using the Affymetrix Human Genome U133+ 2.0 chip with a commercial normal human parietal cortex RNA pool as a normal control. In total, 8,774 genes showed differential expression; among them 2,769 genes were upregulated and 6,005 genes were downregulated. The reliability of the results was confirmed using real-time RT-PCR assays. The most differentially expressed genes (DEGs) were involved in transcription regulation, ion transport, transcription, cell adhesion, and signal transduction. The genes associated with gliosis were upregulated and the genes marked for neurons were downregulated, while the transcription of the PRNP gene remained unaltered. A total of 169 different pathways exhibited significant changes in the brain of G114V gCJD. The most significantly regulated pathways included Alzheimer's and Parkinson's disease, oxidative phosphorylation, regulation of actin cytoskeleton, MAPK signaling and proteasome, which have previously been linked to prion diseases. In addition, we found some pathways that have rarely been explored in regards to prion diseases that were also significantly altered in G114V gCJD, such as axon guidance, gap junction and purine metabolism. The majority of the genes in the 10 most altered pathways were downregulated. The data of the present study provide useful insights into the pathogenesis of G114V gCJD and potential biomarkers for diagnostic and therapeutic purposes.
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March 2013
Volume 31 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Tian C, Liu D, Chen C, Xu Y, Gong H, Chen C, Shi Q, Zhang B, Han J, Dong X, Dong X, et al: Global transcriptional profiling of the postmortem brain of a patient with G114V genetic Creutzfeldt-Jakob disease. Int J Mol Med 31: 676-688, 2013
APA
Tian, C., Liu, D., Chen, C., Xu, Y., Gong, H., Chen, C. ... Dong, X. (2013). Global transcriptional profiling of the postmortem brain of a patient with G114V genetic Creutzfeldt-Jakob disease. International Journal of Molecular Medicine, 31, 676-688. https://doi.org/10.3892/ijmm.2013.1239
MLA
Tian, C., Liu, D., Chen, C., Xu, Y., Gong, H., Chen, C., Shi, Q., Zhang, B., Han, J., Dong, X."Global transcriptional profiling of the postmortem brain of a patient with G114V genetic Creutzfeldt-Jakob disease". International Journal of Molecular Medicine 31.3 (2013): 676-688.
Chicago
Tian, C., Liu, D., Chen, C., Xu, Y., Gong, H., Chen, C., Shi, Q., Zhang, B., Han, J., Dong, X."Global transcriptional profiling of the postmortem brain of a patient with G114V genetic Creutzfeldt-Jakob disease". International Journal of Molecular Medicine 31, no. 3 (2013): 676-688. https://doi.org/10.3892/ijmm.2013.1239