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International Journal of Molecular Medicine
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Article

Toll-like receptor 2 in promoting angiogenesis after acute ischemic injury

  • Authors:
    • Yifei Xu
    • Ying Zhou
    • Haiyan Lin
    • Haiyang Hu
    • Yuxing Wang
    • Geng Xu
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, The Second Affiliated Hospital, Zhejiang University College of Medicine, Hangzhou, Zhejiang 310009, P.R. China
  • Pages: 555-560
    |
    Published online on: January 11, 2013
       https://doi.org/10.3892/ijmm.2013.1240
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Abstract

Angiogenesis is an important mechanism that protects tissue against necrosis following acute ischemic injury. The aim of this study was to investigate whether the Toll-like receptor 2 (TLR2) signaling pathway is involved in angiogenesis following ischemic injury by cell migration and lymphocyte invasion assays in vitro, and a mouse model of hindlimb ischemia by ligation in vivo, respectively. To assess the potential role of TLR2 activation in endothelial cell permeability, HUVECs were pretreated with Pam3CSK4 and analyzed using wound repair and transwell assays. The results showed that the TLR2 agonist induced human umbilical vein endothelial cell (HUVEC) migration and increased the permeability of HUVECs to lymphocyte. The lymphocyte invasion of TLR2 knockout (TLR2-/-) mice was inhibited as compared to that of wild-type (WT) mice. In the mouse model of hindlimb ischemia by ligation, blood perfusion of operated limbs was significantly lower in TLR2-/- compared to WT mice, 7 and 14 days after ligation. TLR2-/- mice showed a decreased CD31 expression in ischemic gastrocnemius at 7 and 14 days after ligation, reduced interleukin-6 (IL-6) level and lowered tumor necrosis factor-α (TNF-α) levels. These findings demonstrated that TLR2 activation promotes cell migration, cell permeability and the lymphocyte invasion of endothelial cells. TLR2 activation promotes angiogenesis in vivo, which may be associated with the serum of TNF-α levels and IL-6 release.
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Copy and paste a formatted citation
Spandidos Publications style
Xu Y, Zhou Y, Lin H, Hu H, Wang Y and Xu G: Toll-like receptor 2 in promoting angiogenesis after acute ischemic injury. Int J Mol Med 31: 555-560, 2013.
APA
Xu, Y., Zhou, Y., Lin, H., Hu, H., Wang, Y., & Xu, G. (2013). Toll-like receptor 2 in promoting angiogenesis after acute ischemic injury. International Journal of Molecular Medicine, 31, 555-560. https://doi.org/10.3892/ijmm.2013.1240
MLA
Xu, Y., Zhou, Y., Lin, H., Hu, H., Wang, Y., Xu, G."Toll-like receptor 2 in promoting angiogenesis after acute ischemic injury". International Journal of Molecular Medicine 31.3 (2013): 555-560.
Chicago
Xu, Y., Zhou, Y., Lin, H., Hu, H., Wang, Y., Xu, G."Toll-like receptor 2 in promoting angiogenesis after acute ischemic injury". International Journal of Molecular Medicine 31, no. 3 (2013): 555-560. https://doi.org/10.3892/ijmm.2013.1240
Copy and paste a formatted citation
x
Spandidos Publications style
Xu Y, Zhou Y, Lin H, Hu H, Wang Y and Xu G: Toll-like receptor 2 in promoting angiogenesis after acute ischemic injury. Int J Mol Med 31: 555-560, 2013.
APA
Xu, Y., Zhou, Y., Lin, H., Hu, H., Wang, Y., & Xu, G. (2013). Toll-like receptor 2 in promoting angiogenesis after acute ischemic injury. International Journal of Molecular Medicine, 31, 555-560. https://doi.org/10.3892/ijmm.2013.1240
MLA
Xu, Y., Zhou, Y., Lin, H., Hu, H., Wang, Y., Xu, G."Toll-like receptor 2 in promoting angiogenesis after acute ischemic injury". International Journal of Molecular Medicine 31.3 (2013): 555-560.
Chicago
Xu, Y., Zhou, Y., Lin, H., Hu, H., Wang, Y., Xu, G."Toll-like receptor 2 in promoting angiogenesis after acute ischemic injury". International Journal of Molecular Medicine 31, no. 3 (2013): 555-560. https://doi.org/10.3892/ijmm.2013.1240
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