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International Journal of Molecular Medicine
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March 2013 Volume 31 Issue 3

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Article

Clinical relevance of plasma miR-106b levels in patients with chronic obstructive pulmonary disease

  • Authors:
    • Seiko Soeda
    • Junko H. Ohyashiki
    • Kazushige Ohtsuki
    • Tomohiro Umezu
    • Yasuhiro Setoguchi
    • Kazuma Ohyashiki
  • View Affiliations / Copyright

    Affiliations: Division of Respiratory Medicine, Tokyo Medical University, Tokyo, Japan, Institute of Medical Science, Tokyo Medical University, Tokyo, Japan, Department of Molecular Science, Tokyo Medical University, Tokyo, Japan
  • Pages: 533-539
    |
    Published online on: January 21, 2013
       https://doi.org/10.3892/ijmm.2013.1251
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Abstract

Chronic obstructive pulmonary disease (COPD) is characterized by both chronic inflammation in the airway and systemic inflammation; however, the molecular mechanism of COPD has not been fully elucidated. By measuring microRNA (miRNA) expression in the plasma of COPD subjects, we aimed to identify the clinical relevance of plasma miRNA levels in these patients. Blood samples were obtained from COPD patients and age-matched normal controls. We initially produced plasma miRNA expression profiles using TaqMan low-density array screening. For further validation, individual qRT-PCRs were performed in 40 COPD patients and 20 healthy subjects. TaqMan low-density array screening showed that 9 miRNAs (miR-29b, miR-483-5p, miR-152, miR-629, miR-26b, miR-101, miR-106b, miR-532-5p and miR-133b) were significantly downregulated in the plasma from COPD patients when compared with normal smokers. Among these miRNAs, we focused on miR-106b. A reduction in the plasma miR-106b levels was evident in COPD ex-smokers and COPD current smokers compared with levels in smokers. There was a negative correlation between the plasma miR-106b level and the duration of disease since diagnosis in COPD ex-smokers and the duration of smoking in COPD current smokers. These findings support the concept that progressive reduction in the plasma miR-106b level may reflect persistent and systemic changes even after the discontinuation of smoking in COPD patients. miR-106b may therefore play an important role in the pathogenesis of COPD.
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Copy and paste a formatted citation
Spandidos Publications style
Soeda S, Ohyashiki JH, Ohtsuki K, Umezu T, Setoguchi Y and Ohyashiki K: Clinical relevance of plasma miR-106b levels in patients with chronic obstructive pulmonary disease. Int J Mol Med 31: 533-539, 2013.
APA
Soeda, S., Ohyashiki, J.H., Ohtsuki, K., Umezu, T., Setoguchi, Y., & Ohyashiki, K. (2013). Clinical relevance of plasma miR-106b levels in patients with chronic obstructive pulmonary disease. International Journal of Molecular Medicine, 31, 533-539. https://doi.org/10.3892/ijmm.2013.1251
MLA
Soeda, S., Ohyashiki, J. H., Ohtsuki, K., Umezu, T., Setoguchi, Y., Ohyashiki, K."Clinical relevance of plasma miR-106b levels in patients with chronic obstructive pulmonary disease". International Journal of Molecular Medicine 31.3 (2013): 533-539.
Chicago
Soeda, S., Ohyashiki, J. H., Ohtsuki, K., Umezu, T., Setoguchi, Y., Ohyashiki, K."Clinical relevance of plasma miR-106b levels in patients with chronic obstructive pulmonary disease". International Journal of Molecular Medicine 31, no. 3 (2013): 533-539. https://doi.org/10.3892/ijmm.2013.1251
Copy and paste a formatted citation
x
Spandidos Publications style
Soeda S, Ohyashiki JH, Ohtsuki K, Umezu T, Setoguchi Y and Ohyashiki K: Clinical relevance of plasma miR-106b levels in patients with chronic obstructive pulmonary disease. Int J Mol Med 31: 533-539, 2013.
APA
Soeda, S., Ohyashiki, J.H., Ohtsuki, K., Umezu, T., Setoguchi, Y., & Ohyashiki, K. (2013). Clinical relevance of plasma miR-106b levels in patients with chronic obstructive pulmonary disease. International Journal of Molecular Medicine, 31, 533-539. https://doi.org/10.3892/ijmm.2013.1251
MLA
Soeda, S., Ohyashiki, J. H., Ohtsuki, K., Umezu, T., Setoguchi, Y., Ohyashiki, K."Clinical relevance of plasma miR-106b levels in patients with chronic obstructive pulmonary disease". International Journal of Molecular Medicine 31.3 (2013): 533-539.
Chicago
Soeda, S., Ohyashiki, J. H., Ohtsuki, K., Umezu, T., Setoguchi, Y., Ohyashiki, K."Clinical relevance of plasma miR-106b levels in patients with chronic obstructive pulmonary disease". International Journal of Molecular Medicine 31, no. 3 (2013): 533-539. https://doi.org/10.3892/ijmm.2013.1251
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