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International Journal of Molecular Medicine
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March 2013 Volume 31 Issue 3

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Article

Serum from hepatectomized rats induces the differentiation of adipose tissue mesenchymal stem cells into hepatocyte-like cells and upregulates the expression of hepatocyte growth factor and interleukin-6 in vitro

  • Authors:
    • Jiangyang Sun
    • Yufeng Yuan
    • Haiquan Qin
    • Chengcheng Ying
    • Weijun Liu
    • Jin Zhang
    • Yueming He
    • Zhisu Liu
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China, Department of Urology Surgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China
  • Pages: 667-675
    |
    Published online on: January 23, 2013
       https://doi.org/10.3892/ijmm.2013.1257
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Abstract

Adipose tissue-derived mesenchymal stem cells (AT-MSCs) are an attractive alternative for clinical application due to their minimally invasive accessibility and availability in the body. However, the hepatic differentiation efficiency of AT-MSCs is insufficient for therapeutic application and the role of extrahepatic stem cells in liver regeneration remains poorly understood. This study was designed to investigate the effects of serum from rats subjected to 70% partial hepatectomy (PH) on the differentiation ability of rat AT-MSCs in vitro, and to explore the potential role of AT-MSCs in vivo following PH injury. Results showed that AT-MSCs treated with serum collected from rats 24 h after 70% PH differen­tiated into hepatocyte-like cells, resembled hepatocyte-like cells with round or polygonal shape, expressed α-fetoprotein, secreted albumin, synthesized urea and acquired cytochrome P450 type 3A4 enzyme activity, and upregulated the expression of interleukin (IL)-6 and hepatocyte growth factor (HGF) transiently in vitro, although the hepatic differentiation efficiency was extremely low. AT-MSC transplantation after 70% PH ameliorated liver injury and promoted liver regeneration, but did not increase the serum levels of IL-6 and HGF in vivo. This result suggests that the therapeutic effect of AT-MSCs in vivo after 24 h of 70% PH does not increase IL-6 and HGF expression.
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Copy and paste a formatted citation
Spandidos Publications style
Sun J, Yuan Y, Qin H, Ying C, Liu W, Zhang J, He Y and Liu Z: Serum from hepatectomized rats induces the differentiation of adipose tissue mesenchymal stem cells into hepatocyte-like cells and upregulates the expression of hepatocyte growth factor and interleukin-6 in vitro. Int J Mol Med 31: 667-675, 2013.
APA
Sun, J., Yuan, Y., Qin, H., Ying, C., Liu, W., Zhang, J. ... Liu, Z. (2013). Serum from hepatectomized rats induces the differentiation of adipose tissue mesenchymal stem cells into hepatocyte-like cells and upregulates the expression of hepatocyte growth factor and interleukin-6 in vitro. International Journal of Molecular Medicine, 31, 667-675. https://doi.org/10.3892/ijmm.2013.1257
MLA
Sun, J., Yuan, Y., Qin, H., Ying, C., Liu, W., Zhang, J., He, Y., Liu, Z."Serum from hepatectomized rats induces the differentiation of adipose tissue mesenchymal stem cells into hepatocyte-like cells and upregulates the expression of hepatocyte growth factor and interleukin-6 in vitro". International Journal of Molecular Medicine 31.3 (2013): 667-675.
Chicago
Sun, J., Yuan, Y., Qin, H., Ying, C., Liu, W., Zhang, J., He, Y., Liu, Z."Serum from hepatectomized rats induces the differentiation of adipose tissue mesenchymal stem cells into hepatocyte-like cells and upregulates the expression of hepatocyte growth factor and interleukin-6 in vitro". International Journal of Molecular Medicine 31, no. 3 (2013): 667-675. https://doi.org/10.3892/ijmm.2013.1257
Copy and paste a formatted citation
x
Spandidos Publications style
Sun J, Yuan Y, Qin H, Ying C, Liu W, Zhang J, He Y and Liu Z: Serum from hepatectomized rats induces the differentiation of adipose tissue mesenchymal stem cells into hepatocyte-like cells and upregulates the expression of hepatocyte growth factor and interleukin-6 in vitro. Int J Mol Med 31: 667-675, 2013.
APA
Sun, J., Yuan, Y., Qin, H., Ying, C., Liu, W., Zhang, J. ... Liu, Z. (2013). Serum from hepatectomized rats induces the differentiation of adipose tissue mesenchymal stem cells into hepatocyte-like cells and upregulates the expression of hepatocyte growth factor and interleukin-6 in vitro. International Journal of Molecular Medicine, 31, 667-675. https://doi.org/10.3892/ijmm.2013.1257
MLA
Sun, J., Yuan, Y., Qin, H., Ying, C., Liu, W., Zhang, J., He, Y., Liu, Z."Serum from hepatectomized rats induces the differentiation of adipose tissue mesenchymal stem cells into hepatocyte-like cells and upregulates the expression of hepatocyte growth factor and interleukin-6 in vitro". International Journal of Molecular Medicine 31.3 (2013): 667-675.
Chicago
Sun, J., Yuan, Y., Qin, H., Ying, C., Liu, W., Zhang, J., He, Y., Liu, Z."Serum from hepatectomized rats induces the differentiation of adipose tissue mesenchymal stem cells into hepatocyte-like cells and upregulates the expression of hepatocyte growth factor and interleukin-6 in vitro". International Journal of Molecular Medicine 31, no. 3 (2013): 667-675. https://doi.org/10.3892/ijmm.2013.1257
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