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International Journal of Molecular Medicine
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April 2013 Volume 31 Issue 4

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Article

Expression profiles of miRNAs in polarized macrophages

  • Authors:
    • Yingying Zhang
    • Mengying Zhang
    • Min Zhong
    • Qifeng Suo
    • Kun Lv
  • View Affiliations / Copyright

    Affiliations: Laboratory Medicine of Yijishan Hospital, Wuhu, Anhui 241001, P.R. China, Central Laboratory of Yijishan Hospital, Wannan Medical College, Wuhu, Anhui 241001, P.R. China
  • Pages: 797-802
    |
    Published online on: January 30, 2013
       https://doi.org/10.3892/ijmm.2013.1260
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Abstract

microRNAs (miRNAs) play a crucial role in tissue development and the pathology of various diseases. However, the effects and roles of miRNAs in macrophage polarization have yet to be investigated. In this study, we analyzed and compared the miRNA expression profiles of bone marrow-derived macrophages (BMDMs) with two distinct polarizing conditions (classical macrophage activation ‘M1’ and alternative activation ‘M2’) using miRNA microarray. In total, 109 miRNAs were differentially expressed between M1 and M2. The differential expression of selected miRNAs was validated by real-time qRT-PCR: miR-181a, miR-155-5p, miR-204-5p and miR-451 were upregulated (fold change >2, P<0.05) and miR-125-5p, miR-146a-3p, miR-143-3p and miR-145-5p were downregulated (fold change <-2, P<0.05) in M1 compared with M2. In conclusion, our study may be useful for exploring the precise roles of miRNAs in macrophage differentiation and polarized activation processes in the future.
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1 

Rosa A, Ballarino M, Sorrentino A, et al: The interplay between the master transcription factor PU1 and miR-424 regulates human monocyte/macrophage differentiation. Proc Natl Acad Sci USA. 104:19849–19854. 2007. View Article : Google Scholar : PubMed/NCBI

2 

Androulidaki A, Iliopoulos D, Arranz A, et al: The kinase Akt1 controls macrophage response to lipopolysaccharide by regulating microRNAs. Immunity. 31:220–231. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Bazzoni F, Rossato M, Fabbri M, et al: Induction and regulatory function of miR-9 in human monocytes and neutrophils exposed to proinflammatory signals. Proc Natl Acad Sci USA. 106:5282–5287. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Du CS, Liu C, Kang JH, et al: MicroRNA miR-326 regulates TH-17 differentiation and is associated with the pathogenesis of multiple sclerosis. Nat Immunol. 10:1252–1259. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Chang CI, Liao JC and Kuo L: Arginase modulates nitric oxide production in activated macrophages. Am J Physiol. 274:H342–H348. 1998.PubMed/NCBI

6 

Chen T, Huang Z, Wang L, Wang Y, Wu F, Meng S and Wang C: MicroRNA-125a-5p partly regulates the inflammatory response lipid uptake and ORP9 expression in oxLDL-stimulated monocyte/macrophages. Cardiovas Res. 83:131–139. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Cheng Y, Kuang WH, Hao YH, et al: Downregulation of miR-27a* and miR-532-5p and upregulation of miR-146a and miR-155 in LPS-induced RAW2647 macrophage cells. Inflammation. 35:1308–1313. 2012.

8 

Coley W, Van Duyne R, Carpio L, et al: Absence of DICER in monocytes and its regulation by HIV-1. J Biol Chem. 285:31930–31943. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Dalton DK, Pitts-Meek S, Keshav IS, Figari A, Bradley A and Stewart TA: Multiple defects of immune cell function in mice with disrupted interferon-gamma genes. Science. 259:1739–1742. 1993. View Article : Google Scholar : PubMed/NCBI

10 

Forrest AR, Kanamori-Katayama M, Tomaru Y, et al: Induction of microRNAs mir-155 mir-222 mir-424 and mir-503 promotes monocytic differentiation through combinatorial regulation. Leukemia. 24:460–466. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Goerdt S and Orfanos CE: Other functions other genes: alternative activation of antigen-presenting cells. Immunity. 10:137–142. 1999. View Article : Google Scholar : PubMed/NCBI

12 

Goerdt S, O Politz K, Schledzewski R, et al: Alternative versus classical activation of macrophages. Pathobiology. 67:222–226. 1999. View Article : Google Scholar : PubMed/NCBI

13 

Gordon S: Alternative activation of macrophages. Nat Rev Immunol. 3:23–35. 2003. View Article : Google Scholar

14 

Graff JW, Dickson AM, Gwendolyn C, McCaffrey AP and Wilson ME: Identifying functional microRNAs in macrophages with polarized phenotypes. J Biol Chem. 287:21816–21825. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Lewis BP, Burge CB and Bartel DP: Conserved seed pairing often flanked by adenosines indicates that thousands of human genes are microRNA targets. Cell. 120:15–20. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Liu G, Friggeri A, Yang Y, Park YJ, Tsuruta Y and Abraham E: miR-147 a microRNA that is induced upon Toll-like receptor stimulation regulates murine macrophage inflammatory responses. Proc Natl Acad Sci USA. 106:15819–15824. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Lu M, Shi B, Wang J, Cao Q and Cui QH: TAM: a method for enrichment and depletion analysis of a microRNA category in a list of microRNAs. BMC Bioinformatics. 11:419–426. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Lumeng CN, Bodzin JL and Saltiel AR: Obesity induces a phenotypic switch in adipose tissue macrophage polarization. J Clin Invest. 117:175–184. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Mallory AC and Vaucheret H: MicroRNAs: something important between the genes. Curr Opin Plant Biol. 7:120–125. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Mantovani A, Sica A and Locati M: Macrophage polarization comes of age. Immunity. 23:344–346. 2005. View Article : Google Scholar : PubMed/NCBI

21 

Mantovani A, Sica A, Sozzani S, Allavena P, Vecchi A and Locati M: The chemokine system in diverse forms of macrophage activation and polarization. Trends Immunol. 25:677–686. 2004. View Article : Google Scholar : PubMed/NCBI

22 

Mantovani A, Sozzani S, Locati M, Allavena P and Sica A: Macrophage polarization: tumor-associated macrophages as a paradigm for polarized M2 mononuclear phagocytes. Trends Immunol. 23:549–555. 2002. View Article : Google Scholar : PubMed/NCBI

23 

Mills CD, Kincaid K, Alt JM, Heilman MJ and Hill AM: M-1/M-2 macrophages and the Th1/Th2 paradigm. J Immunol. 164:6166–6173. 2000. View Article : Google Scholar : PubMed/NCBI

24 

Monk CE, Hutvagner G and Arthur JS: Regulation of miRNA transcription in macrophages in response to Candida albicans. PLoS One. 5:e136692010. View Article : Google Scholar : PubMed/NCBI

25 

Mosser DM: The many faces of macrophage activation. J Leukoc Biol. 73:209–212. 2003. View Article : Google Scholar : PubMed/NCBI

26 

Mosser DM and Edwards JP: Exploring the full spectrum of macrophage activation. Nat Rev Immunol. 8:958–969. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Munder M, Eichmann K and Modolell M: Alternative metabolic states in murine macrophages reflected by the nitric oxide synthase/arginase balance: competitive regulation by CD4+ T cells correlates with Th1/Th2 phenotype. J Immunol. 160:5347–5354. 1998.

28 

Nathan CF, Murray HW, Wiebe ME and Rubin BY: Identification of interferon-gamma as the lymphokine that activates human macrophage oxidative metabolism and antimicrobial activity. J Exp Med. 158:670–689. 1983. View Article : Google Scholar

29 

O’Connell RM, Taganov KD, Boldin MP, Cheng G and Baltimore D: MicroRNA-155 is induced during the macrophage inflammatory response. Proc Natl Acad Sci USA. 104:1604–1609. 2007.PubMed/NCBI

30 

O’Connell RM, Kahn D, Gibson WS, et al: MicroRNA-155 promotes autoimmune inflammation by enhancing inflammatory T cell development. Immunity. 33:607–619. 2010.PubMed/NCBI

31 

Ruggiero T, Trabucchi M, De Santa F, et al: LPS induces KH-type splicing regulatory protein-dependent processing of microRNA-155 precursors in macrophages. FASEB J. 23:2898–2908. 2009. View Article : Google Scholar : PubMed/NCBI

32 

Schulte LN, Eulalio A, Mollenkopf HJ, Reinhardt R and Vogel J: Analysis of the host microRNA response to Salmonella uncovers the control of major cytokines by the let-7 family. EMBO J. 30:1977–1989. 2011. View Article : Google Scholar : PubMed/NCBI

33 

Taganov KD, Boldin MP, Chang KJ and Baltimore D: NF-kappaB-dependent induction of microRNA miR-146 an inhibitor targeted to signaling proteins of innate immune responses. Proc Natl Acad Sci USA. 103:12481–12486. 2006. View Article : Google Scholar : PubMed/NCBI

34 

Tili E, Michaille JJ, Cimino A, et al: Modulation of miR-155 and miR-125b levels following lipopolysaccharide/TNF-alpha stimulation and their possible roles in regulating the response to endotoxin shock. J Immunol. 179:5082–5089. 2007. View Article : Google Scholar : PubMed/NCBI

35 

Tserel L, Runnel T, Kisand K, et al: MicroRNA expression profiles of human blood monocyte-derived dendritic cells and macrophages reveal miR-511 as putative positive regulator of Toll-like receptor 4. J Biol Chem. 286:26487–26495. 2011. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Zhang M, Zhong M, Suo Q and Lv K: Expression profiles of miRNAs in polarized macrophages. Int J Mol Med 31: 797-802, 2013.
APA
Zhang, Y., Zhang, M., Zhong, M., Suo, Q., & Lv, K. (2013). Expression profiles of miRNAs in polarized macrophages. International Journal of Molecular Medicine, 31, 797-802. https://doi.org/10.3892/ijmm.2013.1260
MLA
Zhang, Y., Zhang, M., Zhong, M., Suo, Q., Lv, K."Expression profiles of miRNAs in polarized macrophages". International Journal of Molecular Medicine 31.4 (2013): 797-802.
Chicago
Zhang, Y., Zhang, M., Zhong, M., Suo, Q., Lv, K."Expression profiles of miRNAs in polarized macrophages". International Journal of Molecular Medicine 31, no. 4 (2013): 797-802. https://doi.org/10.3892/ijmm.2013.1260
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Zhang M, Zhong M, Suo Q and Lv K: Expression profiles of miRNAs in polarized macrophages. Int J Mol Med 31: 797-802, 2013.
APA
Zhang, Y., Zhang, M., Zhong, M., Suo, Q., & Lv, K. (2013). Expression profiles of miRNAs in polarized macrophages. International Journal of Molecular Medicine, 31, 797-802. https://doi.org/10.3892/ijmm.2013.1260
MLA
Zhang, Y., Zhang, M., Zhong, M., Suo, Q., Lv, K."Expression profiles of miRNAs in polarized macrophages". International Journal of Molecular Medicine 31.4 (2013): 797-802.
Chicago
Zhang, Y., Zhang, M., Zhong, M., Suo, Q., Lv, K."Expression profiles of miRNAs in polarized macrophages". International Journal of Molecular Medicine 31, no. 4 (2013): 797-802. https://doi.org/10.3892/ijmm.2013.1260
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