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International Journal of Molecular Medicine
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April 2013 Volume 31 Issue 4

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Article

Serum protein isoform profiles indicate the progression of hepatitis C virus-induced liver diseases

  • Authors:
    • Kazushi Sugimoto
    • Katsuya Shiraki
    • Yoshiyuki Takei
    • Masaaki  Ito
    • Tsutomu Nobori
    • Hideaki Suzuki
    • Santha K. Dissanayaka
    • Kohji  Meno
    • Makoto Asashima
    • Kazuhiko Uchida
  • View Affiliations / Copyright

    Affiliations: Department of Molecular and Laboratory Medicine, Mie University School of Medicine, Tsu, Japan, First Department of Internal Medicine, Mie University School of Medicine, Tsu, Japan, Department of Gastroenterology, Mie University School of Medicine, Tsu, Japan, Tsukuba Industrial Liaison and Cooperative Research Center, University of Tsukuba, Tsukuba, Japan, Research Center for Stem Cell Engineering National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan
  • Pages: 943-950
    |
    Published online on: February 4, 2013
       https://doi.org/10.3892/ijmm.2013.1267
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Abstract

Biomarkers that enable an accurate diagnosis of hepatitis C virus (HCV)-induced liver diseases are necessary to prevent subsequent patient morbidity and suffering from the onset of hepatocellular carcinoma (HCC). In particular, the identification of novel biomarkers for liver cirrhosis (LC) will be an important new diagnostic tool since more than 70% of HCV-induced LCs are destined to develop into HCC. In our current study, we performed a search for new serological protein biomarkers of HCV-induced chronic hepatitis (CH), LC and HCC, using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). The disease-affected spots were subsequently identified as isoforms of protein components of haptoglobin, transthyretin, the haptoglobin α-chain and apolipoprotein A-IV (apo A-IV), and in specific instances were significantly reduced in LC (p<0.001) and HCC (p<0.01), compared with CH patients. We further examined these isoforms by receiver operating characteristics (ROC) curve analysis and found that they showed high area under ROC curve (AUC) values of more than 0.8 between CH and LC, suggesting that they are appropriate markers that could be utilized to discriminate LC from CH. In conclusion, protein variants in serum that arise as a result of post-translational modifications prove to be useful biomarkers for the accurate diagnosis of specific liver diseases.
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Copy and paste a formatted citation
Spandidos Publications style
Sugimoto K, Shiraki K, Takei Y, Ito M, Nobori T, Suzuki H, Dissanayaka SK, Meno K, Asashima M, Uchida K, Uchida K, et al: Serum protein isoform profiles indicate the progression of hepatitis C virus-induced liver diseases. Int J Mol Med 31: 943-950, 2013.
APA
Sugimoto, K., Shiraki, K., Takei, Y., Ito, M., Nobori, T., Suzuki, H. ... Uchida, K. (2013). Serum protein isoform profiles indicate the progression of hepatitis C virus-induced liver diseases. International Journal of Molecular Medicine, 31, 943-950. https://doi.org/10.3892/ijmm.2013.1267
MLA
Sugimoto, K., Shiraki, K., Takei, Y., Ito, M., Nobori, T., Suzuki, H., Dissanayaka, S. K., Meno, K., Asashima, M., Uchida, K."Serum protein isoform profiles indicate the progression of hepatitis C virus-induced liver diseases". International Journal of Molecular Medicine 31.4 (2013): 943-950.
Chicago
Sugimoto, K., Shiraki, K., Takei, Y., Ito, M., Nobori, T., Suzuki, H., Dissanayaka, S. K., Meno, K., Asashima, M., Uchida, K."Serum protein isoform profiles indicate the progression of hepatitis C virus-induced liver diseases". International Journal of Molecular Medicine 31, no. 4 (2013): 943-950. https://doi.org/10.3892/ijmm.2013.1267
Copy and paste a formatted citation
x
Spandidos Publications style
Sugimoto K, Shiraki K, Takei Y, Ito M, Nobori T, Suzuki H, Dissanayaka SK, Meno K, Asashima M, Uchida K, Uchida K, et al: Serum protein isoform profiles indicate the progression of hepatitis C virus-induced liver diseases. Int J Mol Med 31: 943-950, 2013.
APA
Sugimoto, K., Shiraki, K., Takei, Y., Ito, M., Nobori, T., Suzuki, H. ... Uchida, K. (2013). Serum protein isoform profiles indicate the progression of hepatitis C virus-induced liver diseases. International Journal of Molecular Medicine, 31, 943-950. https://doi.org/10.3892/ijmm.2013.1267
MLA
Sugimoto, K., Shiraki, K., Takei, Y., Ito, M., Nobori, T., Suzuki, H., Dissanayaka, S. K., Meno, K., Asashima, M., Uchida, K."Serum protein isoform profiles indicate the progression of hepatitis C virus-induced liver diseases". International Journal of Molecular Medicine 31.4 (2013): 943-950.
Chicago
Sugimoto, K., Shiraki, K., Takei, Y., Ito, M., Nobori, T., Suzuki, H., Dissanayaka, S. K., Meno, K., Asashima, M., Uchida, K."Serum protein isoform profiles indicate the progression of hepatitis C virus-induced liver diseases". International Journal of Molecular Medicine 31, no. 4 (2013): 943-950. https://doi.org/10.3892/ijmm.2013.1267
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