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Article

Microarray analyses of peripheral whole blood cells from ulcerative colitis patients: Effects of leukocytapheresis

  • Authors:
    • Teppei Kobayashi
    • Keiichi Mitsuyama
    • Hiroshi Yamasaki
    • Junya Masuda
    • Hidetoshi Takedatsu
    • Kotaro Kuwaki
    • Shinichiro Yoshioka
    • Koji Nagayama
    • Michio Sata
  • View Affiliations / Copyright

    Affiliations: Division of Gastroenterology, Department of Medicine, Kurume University School of Medicine, Kurume 830-0011, Japan
  • Pages: 789-796
    |
    Published online on: February 6, 2013
       https://doi.org/10.3892/ijmm.2013.1270
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Abstract

Complementary DNA microarray technology allows the simultaneous analysis of the expression of hundreds to thousands of genes. We applied this technique to clarify the molecular mechanisms underlying the therapeutic effects of leukocytapheresis (LCAP) therapy in patients with ulcerative colitis (UC). A 776-gene microarray analysis was performed using whole blood cells from six normal subjects and six patients with active UC who had undergone filtration LCAP. Widespread gene upregulation was observed in patients with UC, compared with normal subjects. After LCAP, genes with proinflammatory actions, such as CD97, CD74, human leukocyte antigen-DRβ1 and -DP light chain, were downregulated, while genes responsible for antimicrobial actions, such as neutrophil gelatinase-associated lipocalin, and acute phase reactions, such as haptoglobin α1S and α1-acid glycoprotein, were upregulated. In conclusion, we identified several genes expressed in the whole blood cells of UC patients as well as the transcriptional events following LCAP. Following LCAP, the gene profile shifted toward a pattern indicating disease improvement. These results suggest a basis for the molecular mechanisms leading to the therapeutic effects of LCAP and also indicate new therapeutic targets, providing important prognostic information.
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Copy and paste a formatted citation
Spandidos Publications style
Kobayashi T, Mitsuyama K, Yamasaki H, Masuda J, Takedatsu H, Kuwaki K, Yoshioka S, Nagayama K and Sata M: Microarray analyses of peripheral whole blood cells from ulcerative colitis patients: Effects of leukocytapheresis. Int J Mol Med 31: 789-796, 2013.
APA
Kobayashi, T., Mitsuyama, K., Yamasaki, H., Masuda, J., Takedatsu, H., Kuwaki, K. ... Sata, M. (2013). Microarray analyses of peripheral whole blood cells from ulcerative colitis patients: Effects of leukocytapheresis. International Journal of Molecular Medicine, 31, 789-796. https://doi.org/10.3892/ijmm.2013.1270
MLA
Kobayashi, T., Mitsuyama, K., Yamasaki, H., Masuda, J., Takedatsu, H., Kuwaki, K., Yoshioka, S., Nagayama, K., Sata, M."Microarray analyses of peripheral whole blood cells from ulcerative colitis patients: Effects of leukocytapheresis". International Journal of Molecular Medicine 31.4 (2013): 789-796.
Chicago
Kobayashi, T., Mitsuyama, K., Yamasaki, H., Masuda, J., Takedatsu, H., Kuwaki, K., Yoshioka, S., Nagayama, K., Sata, M."Microarray analyses of peripheral whole blood cells from ulcerative colitis patients: Effects of leukocytapheresis". International Journal of Molecular Medicine 31, no. 4 (2013): 789-796. https://doi.org/10.3892/ijmm.2013.1270
Copy and paste a formatted citation
x
Spandidos Publications style
Kobayashi T, Mitsuyama K, Yamasaki H, Masuda J, Takedatsu H, Kuwaki K, Yoshioka S, Nagayama K and Sata M: Microarray analyses of peripheral whole blood cells from ulcerative colitis patients: Effects of leukocytapheresis. Int J Mol Med 31: 789-796, 2013.
APA
Kobayashi, T., Mitsuyama, K., Yamasaki, H., Masuda, J., Takedatsu, H., Kuwaki, K. ... Sata, M. (2013). Microarray analyses of peripheral whole blood cells from ulcerative colitis patients: Effects of leukocytapheresis. International Journal of Molecular Medicine, 31, 789-796. https://doi.org/10.3892/ijmm.2013.1270
MLA
Kobayashi, T., Mitsuyama, K., Yamasaki, H., Masuda, J., Takedatsu, H., Kuwaki, K., Yoshioka, S., Nagayama, K., Sata, M."Microarray analyses of peripheral whole blood cells from ulcerative colitis patients: Effects of leukocytapheresis". International Journal of Molecular Medicine 31.4 (2013): 789-796.
Chicago
Kobayashi, T., Mitsuyama, K., Yamasaki, H., Masuda, J., Takedatsu, H., Kuwaki, K., Yoshioka, S., Nagayama, K., Sata, M."Microarray analyses of peripheral whole blood cells from ulcerative colitis patients: Effects of leukocytapheresis". International Journal of Molecular Medicine 31, no. 4 (2013): 789-796. https://doi.org/10.3892/ijmm.2013.1270
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