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International Journal of Molecular Medicine
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April 2013 Volume 31 Issue 4

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Article

EBM84 attenuates airway inflammation and mucus hypersecretion in an ovalbumin-induced murine model of asthma

  • Authors:
    • In Sik Shin
    • Mee Young Lee
    • Woo Young Jeon
    • Na Ra Shin
    • Chang Seob Seo
    • Hyekyung Ha
  • View Affiliations / Copyright

    Affiliations: Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine, Yusung-gu, Daejeon 305-811, Republic of Korea
  • Pages: 982-988
    |
    Published online on: February 6, 2013
       https://doi.org/10.3892/ijmm.2013.1273
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Abstract

EBM84 is a traditional herbal medicine and a combination of extracts obtained from Pinellia ternata and Zingiber officinale. It is traditionally used to treat vomiting, nausea, sputum and gastrointestinal disorders, and functions is an effective expectorant. In this study, we evaluated the protective effects of EBM84 on asthmatic responses, particularly mucus hypersecretion in an ovalbumin (OVA)-induced murine model of asthma. We also analyzed EBM84 composition using high performance liquid chromatography. Animals were sensitized on days 0 and 14 via intraperitoneal injection using 20 µg OVA. On days 21, 22 and 23 after initial sensitization, the mice received an airway challenge with OVA (1% w/v in PBS) for 1 h using an ultrasonic nebulizer (NE-U12). EBM84 was administered by gavage to the mice at doses of 16.9, 33.8 and 67.5 mg/kg once daily from days 18 to 23. EBM84 administration significantly lowered elevated levels of interleukin (IL)-4, IL-13, eotaxin and immunoglobulin (Ig)E in the bronchoalveolar lavage fluid or plasma. Airway inflammation and mucus hypersecretion were attenuated following EBM84 administration. EBM84 also inhibited the overexpression of mucin 5AC (MUC5AC) induced by OVA challenge in lung tissue. This result was consistent with the immunohistochemistry results. Our results indicate that EBM84 effectively inhibited airway inflammation and mucus hypersecretion via the downregulation of T helper 2 (Th2) cytokines, which reduced MUC5AC expression. Therefore, EBM84 has potential as a useful medicine for the treatment of allergic asthma.
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Copy and paste a formatted citation
Spandidos Publications style
Shin IS, Lee MY, Jeon WY, Shin NR, Seo CS and Ha H: EBM84 attenuates airway inflammation and mucus hypersecretion in an ovalbumin-induced murine model of asthma. Int J Mol Med 31: 982-988, 2013.
APA
Shin, I.S., Lee, M.Y., Jeon, W.Y., Shin, N.R., Seo, C.S., & Ha, H. (2013). EBM84 attenuates airway inflammation and mucus hypersecretion in an ovalbumin-induced murine model of asthma. International Journal of Molecular Medicine, 31, 982-988. https://doi.org/10.3892/ijmm.2013.1273
MLA
Shin, I. S., Lee, M. Y., Jeon, W. Y., Shin, N. R., Seo, C. S., Ha, H."EBM84 attenuates airway inflammation and mucus hypersecretion in an ovalbumin-induced murine model of asthma". International Journal of Molecular Medicine 31.4 (2013): 982-988.
Chicago
Shin, I. S., Lee, M. Y., Jeon, W. Y., Shin, N. R., Seo, C. S., Ha, H."EBM84 attenuates airway inflammation and mucus hypersecretion in an ovalbumin-induced murine model of asthma". International Journal of Molecular Medicine 31, no. 4 (2013): 982-988. https://doi.org/10.3892/ijmm.2013.1273
Copy and paste a formatted citation
x
Spandidos Publications style
Shin IS, Lee MY, Jeon WY, Shin NR, Seo CS and Ha H: EBM84 attenuates airway inflammation and mucus hypersecretion in an ovalbumin-induced murine model of asthma. Int J Mol Med 31: 982-988, 2013.
APA
Shin, I.S., Lee, M.Y., Jeon, W.Y., Shin, N.R., Seo, C.S., & Ha, H. (2013). EBM84 attenuates airway inflammation and mucus hypersecretion in an ovalbumin-induced murine model of asthma. International Journal of Molecular Medicine, 31, 982-988. https://doi.org/10.3892/ijmm.2013.1273
MLA
Shin, I. S., Lee, M. Y., Jeon, W. Y., Shin, N. R., Seo, C. S., Ha, H."EBM84 attenuates airway inflammation and mucus hypersecretion in an ovalbumin-induced murine model of asthma". International Journal of Molecular Medicine 31.4 (2013): 982-988.
Chicago
Shin, I. S., Lee, M. Y., Jeon, W. Y., Shin, N. R., Seo, C. S., Ha, H."EBM84 attenuates airway inflammation and mucus hypersecretion in an ovalbumin-induced murine model of asthma". International Journal of Molecular Medicine 31, no. 4 (2013): 982-988. https://doi.org/10.3892/ijmm.2013.1273
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